去势抑郁模型大鼠海马区AQP-4的表达及意义
摘要
目的探讨大鼠去势合并慢性不可预见刺激后海马区AQP-4的变化,阐明AQP-4在围绝经期抑郁模型疾病的作用。方法实验大鼠随机分为假手术组、去势组、去势+CUMS 3组,观察应激前后糖摄入量及open-field抑郁模型行为学改变情况,第23天采用免疫组化及Western Blot法检测AQP-4在大鼠海马区含量表达。结果去势组及去势+CUMS组大鼠糖摄入量减少,行为学表现为抑郁样表现,大鼠海马区免疫组化及Western Blot中AQP-4表达去势组较假手术组增高(P<0.05),尤其去势+CUMS组较假手术组显著增高(P<0.01),去势+CUMS组较去势组增高(P<0.05)。结论大鼠去势术后影响AQP-4表达,而去势+CUMS联合则加重抑郁样表现,增加AQP-4表达,AQP-4与去势抑郁模型大鼠发病密切相关,AQP-4为围绝经期抑郁症患者治疗及诊断提供了的新方向及靶点。
引文
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[2]Saadoun S,Papadopoulos MC,Watanabe H,et al.Involvement of aquaporin-4 in astroglial cell migration and glial scar formation[J].J Cell Sci,2005,118(Pt 24):5691-5698.
[3]徐仟,孙振荣,李桂林,等.人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4和内向整流性钾离子通道4.1的再分布[J].中国康复理论与实践,2012,18(3):215-218.
[4]任爱华,王海鹏,赵华,等.水通道蛋白-4在抑郁及抗抑郁大鼠模型海马区的表达[J].中国老年学杂志,2015,35(7):1913-1915.
[5]Strekalova T,Gorenkova N,Schunk E,et al.Selective effects of citalopram in a mouse model of stress-induced anhedonia with a control for chronic stress[J].Behav Pharmacol,2006,17(3):271-287.
[6]杨婵娟,安瑞娣,杨俊卿,等.造模时间对慢性不可预见性刺激致大鼠抑郁模型的影响[J].四川动物,2015,34(3):383-388.
[7]徐文炜.雌激素与女性抑郁症研究进展[J].国外医学.精神病学分册,2005,32(1):4-6.
[8]Lee S,Jeong J,Kwak Y,et al.Depression research:where are now?[J].Mol Brain,2010,3(1):8.
[9]王睿,刘吉成,罗春娟,等.葛根素对围绝经期抑郁症模型小鼠的神经保护作用及机制研究[J].神经解剖学杂志,2017,33(2):190-196.
[10]张慎忠,章静,高琳,等.AQP4参与卵巢激素对单胺类神经递质的调节作用[J].南京医科大学学报(自然科学版),2007,52(1):15-18.
[11]Keefe DL.Sex hormones and neural mechanisms[J].Arch Sex Behav,2002,31(1):401-403.
[12]Donahue JE,Stopa EG,Chorsky RL,et al.Cells containing immunoreactive estrogen receptor-alpha in the human basal forebrain[J].Brain Res,2000,856(1-2):142-151.
[13]Rozovsky I,Wei M,Stone DJ,et al.Estradiol(E2)enhances neurite outgrowth by repressing glial fibrillaryacidic protein expression and reorganizing laminin[J].Endocrinology,2002,143(2):636-646.