口腔鳞状细胞癌中MAPK8基因的表达及意义
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摘要
目的:研究丝裂原活化蛋白激酶8(MAPK8)基因在口腔鳞状细胞癌中的表达情况及其与癌症患者临床特征之间的关系。方法:收集17例口腔正常黏膜组织和42例口腔鳞状细胞癌(OSCC)组织,利用实时荧光定量聚合酶链反应(Real-timePCR)检测MAPK8 mRNA的相对表达情况,利用免疫组织化学SP技术检测MAPK8蛋白的表达情况。结果:与正常口腔黏膜组织比较,MAPK8 mRNA和蛋白在OSCC中高表达(P <0. 050),不同分化程度(P <0. 050)、不同临床分期(P <0. 050),组间差异具有统计学意义,不同年龄(P>0. 050)、不同性别(P> 0. 050)组间差异无统计学意义。结论:MAPK8基因表达的增高可能在OSCC增殖过程中起到重要作用。
Objective: To study the the expression and significance of MAPK8 gene in oral squamous cell carcinoma( OSCC).Methods: 17 cases of oral normal tissues and 42 of OSCC tissues were collected. The relative expression of MAPK8 mRNA was detected by Real-Time PCR,the expression of MAPK8 protein was detected by immunohistochemical SP method. Results: Over expression of MAPK8 mRNA and protein was observed in OSCC( P < 0. 05),the expression was correlated with the differentiation( P < 0. 05)and clinical stage( P < 0. 05) of the lesion,but not with the age( P > 0. 05) and gender( P > 0. 05). Conclusion: MAPK8 gene may play an important role in the development of OSCC.
Objective: To study the the expression and significance of MAPK8 gene in oral squamous cell carcinoma( OSCC).Methods: 17 cases of oral normal tissues and 42 of OSCC tissues were collected. The relative expression of MAPK8 mRNA was detected by Real-Time PCR,the expression of MAPK8 protein was detected by immunohistochemical SP method. Results: Over expression of MAPK8 mRNA and protein was observed in OSCC( P < 0. 05),the expression was correlated with the differentiation( P < 0. 05)and clinical stage( P < 0. 05) of the lesion,but not with the age( P > 0. 05) and gender( P > 0. 05). Conclusion: MAPK8 gene may play an important role in the development of OSCC.
引文
[1]景新颖,汤晓飞.尼古丁在口腔癌发病作用中的分子机制[J].北京口腔医学,2016,24(3):174-177.
[2]Teh MT,Hutchison IL,Costea DE,et al.Exploiting FOXM1-orchestrated molecular network for early squamous cell carcinoma diagnosis and prognosis[J].Int J Cancer,2013,132(9):2095-2106.
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[4]Lu Y,Wei G,Liu L,et al.Direct targeting of MAPK8IP1by miR-10a-5p is a major mechanism for gastric cancer metastasis[J].Oncol Lett,2017,13(3):1131-1136.
[5]Show MD,Hill CM,Anway MD,et al.Phosphorylation of mitogen-activated protein kinase 8(MAPK8)is associated with germ cell apoptosis and redistribution of the Bcl2-modifying factor(BMF)[J].J Androl,2008,29(3):338-344.
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[7]Khodayari A,Ghaderian SM,Jafarian M,et al.Evaluation of PKM2 and MAPK8IP2 polymorphism in ameloblastic carcinoma:A retrospective quantitative study[J].Int J Mol Cell Med,2012,1(4):203-209.
[8]金子林,郭锡熔,周旭华,等.MAPK8基因在小鼠源性3T3-L1脂肪细胞诱导分化中表达水平的变化[J].中国儿童保健杂志,2004,12(4):327-329.
[9]李国东,蔡郑东,张寅权,等.丝裂原活化蛋白激酶信号通路相关基因在人骨肉瘤中的表达[J].中华肿瘤杂志,2009,31(5):340-345.
[10]Tiwari N,Meyer-Schaller N,Arnold P,et al.Klf4 is a transcriptional regulator of genes critical for EMT,including Jnk1(Mapk8)[J].PLo S One,2013,8(2):e57329.
[11]Slattery ML,Lundgreen A,Wolff RK.MAP kinase genes and colon and rectal cancer[J].Carcinogenesis,2012,33(12):2398-2408.
[2]Teh MT,Hutchison IL,Costea DE,et al.Exploiting FOXM1-orchestrated molecular network for early squamous cell carcinoma diagnosis and prognosis[J].Int J Cancer,2013,132(9):2095-2106.
[3]Wangsa D,Chowdhury SA,Ryott M,et al.Phylogenetic analysis of multiple FISH markers in oral tongue squamous cell carcinoma suggests that a diverse distribution of copy number changes is associated with poor prognosis[J].Int JCancer,2016,138(1):98-109.
[4]Lu Y,Wei G,Liu L,et al.Direct targeting of MAPK8IP1by miR-10a-5p is a major mechanism for gastric cancer metastasis[J].Oncol Lett,2017,13(3):1131-1136.
[5]Show MD,Hill CM,Anway MD,et al.Phosphorylation of mitogen-activated protein kinase 8(MAPK8)is associated with germ cell apoptosis and redistribution of the Bcl2-modifying factor(BMF)[J].J Androl,2008,29(3):338-344.
[6]王文,任玲,王健楠.MAPK信号通路与细胞凋亡的关系[J].中国实用医药,2010,5(15):260-261.
[7]Khodayari A,Ghaderian SM,Jafarian M,et al.Evaluation of PKM2 and MAPK8IP2 polymorphism in ameloblastic carcinoma:A retrospective quantitative study[J].Int J Mol Cell Med,2012,1(4):203-209.
[8]金子林,郭锡熔,周旭华,等.MAPK8基因在小鼠源性3T3-L1脂肪细胞诱导分化中表达水平的变化[J].中国儿童保健杂志,2004,12(4):327-329.
[9]李国东,蔡郑东,张寅权,等.丝裂原活化蛋白激酶信号通路相关基因在人骨肉瘤中的表达[J].中华肿瘤杂志,2009,31(5):340-345.
[10]Tiwari N,Meyer-Schaller N,Arnold P,et al.Klf4 is a transcriptional regulator of genes critical for EMT,including Jnk1(Mapk8)[J].PLo S One,2013,8(2):e57329.
[11]Slattery ML,Lundgreen A,Wolff RK.MAP kinase genes and colon and rectal cancer[J].Carcinogenesis,2012,33(12):2398-2408.