米槁根际土壤真菌多样性及其与果实药用活性成分含量的相关性分析
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摘要
为了解不同产地米槁Cinnamomum migao根际土壤真菌多样性及其差异,探讨米槁活性成分、土壤理化因子与其土壤真菌群落的联系。本文采用高通量测序技术对云南、贵州及广西的9个产点米槁根际土壤真菌群落组成和结构进行分析,测定不同产地米槁的主要活性成分及土壤理化因子,同时对米槁活性成分及其与土壤理化因子和根际土壤真菌种群多样性进行相关性分析。结果表明,米槁根际土壤真菌物种可归入8门、30纲、89目、200科、440属,优势类群为一未能分类的属,相对丰度为18.76%,其次为被孢霉属Mortierella(16.37%)等;不同产地米槁根际土壤真菌群落组成存在差异,最高多样性指数出现在贵州罗甸,随后依次为贵州望谟、广西乐业、广西天峨、贵州册亨、贵州荔波、云南富宁、云南那坡、贵州贞丰;总体而言,广西乐业、贵州望谟和广西天峨3地米槁果实样本活性成分1,8-桉叶素(1,8-cineole)、香桧烯(sabinene)、柠檬烯(limonene)和α-松油醇(α-terpineo)的含量均高于其他样本;冗余分析(redundancy analysis,RDA)结果表明,部分真菌类群与米槁药用活性成分呈正相关。
The correlation between rhizosphere soil fungal communities and medicinal active ingredients in fruits of Cinnamomum migao was investigated. High throughput sequencing technology was used to investigate the fungal microbiota in rhizosphere soil samples collected in nine C. migao-growing locations of Yunnan, Guizhou and Guangxi provinces. The major active compounds in cinnamon fruits and the rhizosphere soil physicochemical factors of the plants were identified. The results showed that the rhizosphere soil fungal species of C. migao were abundant, including 8 phyla, 30 classes, 89 orders, 200 families and 440 genera. The dominant group was an unidentified fungal genus with a relative abundance of 18.76%, followed by Mortierella(16.37%), Cryptococcus(12.44%), Russula(9.45%), etc. The highest fungal diversity index occurred in soil samples from Luodian(LD) of Guizhou, while the diversity index reduced successively in those from Wangmo(WM) of Guizhou, Leye(LY) of Guangxi, Tiane(TE) of Guangxi, Ceheng(CH) of Guizhou, Libo(LB) of Guizhou, Funing(FN) of Yunnan, Napo(NP) of Yunnan, and Zhenfeng(ZF) of Guizhou. The content of active medicinal components, 1,8-cineole, sabinene, limonene and α-terpineo, in LY, WM and TE samples of cinnamon fruits was higher than that in other samples. Redundancy analysis(RDA) showed that diversity of some soil fungi positively influenced the content of active medicinal ingredients of C. migao fruits.
The correlation between rhizosphere soil fungal communities and medicinal active ingredients in fruits of Cinnamomum migao was investigated. High throughput sequencing technology was used to investigate the fungal microbiota in rhizosphere soil samples collected in nine C. migao-growing locations of Yunnan, Guizhou and Guangxi provinces. The major active compounds in cinnamon fruits and the rhizosphere soil physicochemical factors of the plants were identified. The results showed that the rhizosphere soil fungal species of C. migao were abundant, including 8 phyla, 30 classes, 89 orders, 200 families and 440 genera. The dominant group was an unidentified fungal genus with a relative abundance of 18.76%, followed by Mortierella(16.37%), Cryptococcus(12.44%), Russula(9.45%), etc. The highest fungal diversity index occurred in soil samples from Luodian(LD) of Guizhou, while the diversity index reduced successively in those from Wangmo(WM) of Guizhou, Leye(LY) of Guangxi, Tiane(TE) of Guangxi, Ceheng(CH) of Guizhou, Libo(LB) of Guizhou, Funing(FN) of Yunnan, Napo(NP) of Yunnan, and Zhenfeng(ZF) of Guizhou. The content of active medicinal components, 1,8-cineole, sabinene, limonene and α-terpineo, in LY, WM and TE samples of cinnamon fruits was higher than that in other samples. Redundancy analysis(RDA) showed that diversity of some soil fungi positively influenced the content of active medicinal ingredients of C. migao fruits.
引文
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Huang W,Long C,Lam E,2018.Roles of plant-associated microbiota in traditional herbal medicine.Trends in Plant Science,23(7):559-562
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Wang SW,Wang SQ,Li XP,Zhang QF,Ma ZK,Tang HP,2018.Progress in molecular identification in the genus Fusarium and its important applications.Microbiology China,45(4):907-919(in Chinese)
Xiao YH,Li J,Liu ZX,Li YJ,Li G,Long H,2013.Advances in studies on rhizospheric microorganism of medicinal plants.Chinese Traditional and Herbal Drugs,44(4):497-504(in Chinese)
Yang J,Dong CB,Zhang ZY,Liang JD,Han YF,Liang ZQ,2019.Difference analysis of fungal community composition of Eucommia ulmoides rhizosphere soil in different areas.Mycosystema,38(3):327-340(in Chinese)
Yu M,Jiang JL,Ren XM,Li l,Jiao CJ,Yang LJ,Xu H,2018.Research on relationship between occurrence of root rot and changes of fungal communities in rhizosphere of Panax quinquefolius.China Journal of Chinese Materia Medica,43(10):2038-2047(in Chinese)
Zhang HF,Xiao RF,Chen HP,Yuan CQ,Liu B,2018.Growth-promoting effects of non-pathogenic Fusarium oxysporum as biocontrol agent on tomato plant.Fujian Agricultural Science and Technology,2018(11):22-25(in Chinese)
Zhang XB,Zhou T,Guo LP,Huang LQ,Jiang WK,Yang ZN,Ma CY,2011.Volatile oil contents correlate with geographical distribution patterns of the Miao ethnic herb fructus cinnamomi.Acta Ecologica Sinica,31(18):5299-5306(in Chinese)
Zheng H,Zhang ZY,Han YF,Chen WH,Liang ZQ,2018.Community composition and ecological functional structural analysis of the endophytic fungi in Robinia pseudoacacia.Mycosystema,37(2):256-263(in Chinese)
Zheng Y,Liu W,Huang JX,Hou YR,2016.Rhizosphere microorganism evidence on geo-authentic features of traditional Chinese medicine from moutan(Paeonia suffruticosa).Acta Pharmaceutica Sinica,51(8):1325-1333(in Chinese)
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陈娟,朱军,阎波,李佳梅,郭顺星,2018.新疆药用植物天山雪莲及红景天内生真菌的分离与初步鉴定.菌物学报,37(1):110-119
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戴玮,肖苏萍,郭凯,王继永,赵润怀,2013.GC测定苗药大果木姜子中1,8-桉叶素的含量.中国现代中药,15(9):741-743
全妙华,佘朝文,陈东明,胡朝暾,赵丽娟,2018.基于高通量测序的两种典型忽地笑栽培土壤根际真菌群落多样性.微生物学通报,45(10):41-47
李佳,刘济明,文爱华,邓明明,熊雪,柳嘉佳,2019.米槁幼苗光合作用及光响应曲线模拟对干旱胁迫的响应.生态学报,39(3):913-922
李越鲲,孙燕飞,雷勇辉,周旋,尹跃,秦垦,2017.枸杞根际土壤真菌群落多样性的高通量测序.微生物学报,57(7):1049-1059
刘同祥,刘庆山,申刚义,朴香兰,崔箭,2010.大果木姜子镇痛作用活性部位筛选.北京中医药大学学报,33(8):550-554
柳嘉佳,刘济明,王军才,熊雪,李丽霞,骆畅,2017.不同氮素水平对米槁幼苗生长和生理特性的影响.南方农业学报,48(10):1863-1869
邱德文,李鸿玉,赵山,刘宁,刘正仁,1993.米槁的本草学研究.中国医药学报,8(2):19-20
沈丽,马琳,朱海燕,杨小生,2011.大果木姜子的化学成分.中国实验方剂学杂志,17(15):108-109
王举涛,张培良,王刚,王国凯,刘劲松,许凤清,刘海涛,吴培云,2018.凤丹内生真菌Fusarium oxysporum次生代谢产物的研究.中草药,49(22):5247-5253
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张小波,周涛,郭兰萍,黄璐琦,江维克,杨占南,马超一,2011.苗药大果木姜子挥发油成分变化及其地理分布.生态学报,31(18):5299-5306
郑欢,张芝元,韩燕峰,陈万浩,梁宗琦,2018.刺槐内生真菌群落组成及其生态功能结构分析.菌物学报,37(2):256-263
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Barnard RL,Osborne CA,Firestone MK,2013.Responses of soil bacterial and fungal communities to extreme desiccation and rewetting.ISME Journal,7(11):2229-2241
Berendsen RL,Pieterse CMJ,Bakker P,2012.The rhizosphere microbiome and plant health.Trends in Plant Science,17(8):478-486
Berg G,Smalla K,2009.Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere.FEMSMicrobiology Ecology,68(1):1-13
Chen J,Zhu J,Yan B,Li JM,Guo SX,2018.Preliminary identification of endophytic fungi colonized in the root of Saussurea involucrata and Rhodiola rosea from Xinjiang region.Mycosystema,37(1):110-119(in Chinese)
Chen LH,Yan W,2003.The effects of fairy ring of Agaricus arvensis of grassland on the growth of herbage.Chinese Bulletin of Botany,20(1):94-97(in Chinese)
Chen ML,Zhou T,Jiang WK,Jin YL,Yang ZN,2011.Researches on relationship between genetic differentiation and chemical variation of Cinnamomum migao.China Journal of Chinese Materia Medica,36(11):1409-1415(in Chinese)
Dai W,Xiao SP,Guo K,Wang JY,Zhao RH,2013.Determination of 1,8-cineole in the Miao ethnic herb Cinnamomum migao H.W.Li by gas chromatography.Modern Chinese Medicine,15(9):741-743(in Chinese)
Ferlian O,Cesarz S,Craven D,Hines J,Barry KE,Bruelheide H,Buscot F,Haider S,Heklau H,Herrmann S,Kühn P,Pruschitzki U,Sch?dler M,Cameron W,Weigelt A,Wubet T,Eisenhauer N,2018.Mycorrhiza in tree diversity-ecosystem function relationships:conceptual framework and experimental implementation.Ecosphere,9(5):e02226
Huang LQ,Guo LP,Hu J,Shao AJ,2008.molecular mechanism and genetic basis of geoherbs.China Journal of Chinese Materia Medica,33(20):2303-2308(in Chinese)
Huang W,Long C,Lam E,2018.Roles of plant-associated microbiota in traditional herbal medicine.Trends in Plant Science,23(7):559-562
Li J,Liu JM,Wen AH,Deng MM,Xiong X,Liu JJ,2019.Simulated photo synthetic responses of Cinnamomum migao during drought stress evaluated using light-response models.Acta Ecologica Sinica,39(3):913-922(in Chinese)
Li YK,Sun YF,Lei YH,Zhou X,Yin Y,Qin K,2017.Fungal community diversity in rhizosphere soil of Lycium barbarum L.based on high-throughput sequencing.Acta Microbiologica Sinica,57(7):1049-1059(in Chinese)
Liu JJ,Liu JM,Wang JC,Xiong X,Li LX,Luo C,2017.Effects of different nitrogen concentrations on growth and physiological characteristics of Cinnamomum migao seedlings.Guangxi Agricultural Sciences,48(10):1863-1869(in Chinese)
Liu TX,Liu QS,Shen GY,Pu XL,Cui J,2010.Filtration of active fractions with analgesic effect from daguomujiangzi(Cinnamomum migao).Journal of Beijing University of Traditional Chinese Medicine,33(8):550-554(in Chinese)
Mendes R,Garbeva P,Raaijmakers JM,2013.The rhizosphere microbiome:significance of plant beneficial,plant pathogenic,and human pathogenic microorganisms.FEMS Microbiology Reviews,37(5):634-663
Mendes R,Kruijt M,De BI,Dekkers E,Van dVM,Schneider JHM,Piceno YM,Desantis TZ,Andersen GL,Bakker pAHM,Raaijmakers JM,2011.Deciphering the rhizosphere microbiome for disease-suppressive bacteria.Science,332(6033):1097-1100
Powell JR,Rillig MC,2018.Biodiversity of arbuscular mycorrhizal fungi and ecosystem function.New Phytologist,220(4):1059-1075
Qiu DW,Li HY,Zhao S,Liu Y,Liu ZY,1993.A herbal study on mi-gao.China Journal of Traditional Chinese Medicine and Pharmacy,8(2):19-20(in Chinese)
Quan MH,She CW,Chen DM,Hu CT,Zhao LJ,2018.Fungal diversity in two typical types of rhizosphere cultivation soil of Lycoris aurea based on high-throughput sequencing.Microbiology China,45(10):41-47(in Chinese)
Shen L,Ma L,Zhu HY,Yang XS,2011.Study on chemical constituents of Cinnamomum migao.Chinese Journal of Experimental Traditional Medical Formulae,17(15):108-109(in Chinese)
Singh BK,Trivedi P,Singh S,Macdonald CA,Verma JP,2018.Emerging microbiome technologies for sustainable increase in farm productivity and environmental security.Microbiology Australia,39(1):17-23
Wang JT,Zhang PL,Wang G,Wang GK,Liu JS,Xu FQ,Liu HT,Wu PY,2018.Secondary metabolites from endophytic fungus Fusarium oxysporum isolated from Paeonia ostii.Chinese Traditional and Herbal Drugs,49(22):5247-5253(in Chinese)
Wang SW,Wang SQ,Li XP,Zhang QF,Ma ZK,Tang HP,2018.Progress in molecular identification in the genus Fusarium and its important applications.Microbiology China,45(4):907-919(in Chinese)
Xiao YH,Li J,Liu ZX,Li YJ,Li G,Long H,2013.Advances in studies on rhizospheric microorganism of medicinal plants.Chinese Traditional and Herbal Drugs,44(4):497-504(in Chinese)
Yang J,Dong CB,Zhang ZY,Liang JD,Han YF,Liang ZQ,2019.Difference analysis of fungal community composition of Eucommia ulmoides rhizosphere soil in different areas.Mycosystema,38(3):327-340(in Chinese)
Yu M,Jiang JL,Ren XM,Li l,Jiao CJ,Yang LJ,Xu H,2018.Research on relationship between occurrence of root rot and changes of fungal communities in rhizosphere of Panax quinquefolius.China Journal of Chinese Materia Medica,43(10):2038-2047(in Chinese)
Zhang HF,Xiao RF,Chen HP,Yuan CQ,Liu B,2018.Growth-promoting effects of non-pathogenic Fusarium oxysporum as biocontrol agent on tomato plant.Fujian Agricultural Science and Technology,2018(11):22-25(in Chinese)
Zhang XB,Zhou T,Guo LP,Huang LQ,Jiang WK,Yang ZN,Ma CY,2011.Volatile oil contents correlate with geographical distribution patterns of the Miao ethnic herb fructus cinnamomi.Acta Ecologica Sinica,31(18):5299-5306(in Chinese)
Zheng H,Zhang ZY,Han YF,Chen WH,Liang ZQ,2018.Community composition and ecological functional structural analysis of the endophytic fungi in Robinia pseudoacacia.Mycosystema,37(2):256-263(in Chinese)
Zheng Y,Liu W,Huang JX,Hou YR,2016.Rhizosphere microorganism evidence on geo-authentic features of traditional Chinese medicine from moutan(Paeonia suffruticosa).Acta Pharmaceutica Sinica,51(8):1325-1333(in Chinese)
鲍士旦,2000.土壤农化分析.北京:中国农业出版社.1-495
陈娟,朱军,阎波,李佳梅,郭顺星,2018.新疆药用植物天山雪莲及红景天内生真菌的分离与初步鉴定.菌物学报,37(1):110-119
陈立红,闫伟,2003.草原黑蘑(Agaricus arvensis)圈对牧草生产的影响.植物学通报,20(1):94-97
陈美兰,周涛,江维克,金艳蕾,杨占南,2011.苗药大果木姜子的遗传分化及其化学变异的相关性分析.中国中药杂志,36(11):1409-1415
戴玮,肖苏萍,郭凯,王继永,赵润怀,2013.GC测定苗药大果木姜子中1,8-桉叶素的含量.中国现代中药,15(9):741-743
全妙华,佘朝文,陈东明,胡朝暾,赵丽娟,2018.基于高通量测序的两种典型忽地笑栽培土壤根际真菌群落多样性.微生物学通报,45(10):41-47
李佳,刘济明,文爱华,邓明明,熊雪,柳嘉佳,2019.米槁幼苗光合作用及光响应曲线模拟对干旱胁迫的响应.生态学报,39(3):913-922
李越鲲,孙燕飞,雷勇辉,周旋,尹跃,秦垦,2017.枸杞根际土壤真菌群落多样性的高通量测序.微生物学报,57(7):1049-1059
刘同祥,刘庆山,申刚义,朴香兰,崔箭,2010.大果木姜子镇痛作用活性部位筛选.北京中医药大学学报,33(8):550-554
柳嘉佳,刘济明,王军才,熊雪,李丽霞,骆畅,2017.不同氮素水平对米槁幼苗生长和生理特性的影响.南方农业学报,48(10):1863-1869
邱德文,李鸿玉,赵山,刘宁,刘正仁,1993.米槁的本草学研究.中国医药学报,8(2):19-20
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