载阿奇霉素CS/PLGA缓释材料的制备与性能研究
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摘要
以壳聚糖(CS)、乳酸一羟基乙酸共聚物(PLGA)、阿奇霉素片为原料制备多孔载阿奇霉素CS/PLGA缓释材料。采用比表面及孔径分析仪(BET)、场发射扫描电子显微镜(FESEM)、热重-质谱联用仪(TG-MS)、紫外分光光度计(UV)等方法对多孔材料进行表征。BET结果表明所制的多孔材料平均孔直径均在2~50nm范围内,符合多孔材料的要求; FESEM结果表明多孔材料载药成功; TG-MS热分析结果表明,在500℃时,载阿奇霉素多孔材料的质量残留率为33. 8%,阿奇霉素的质量残留率为14. 2%,载阿奇霉素多孔材料热性能优于阿奇霉素药物本身; UV实验测定结果为该缓释剂的载药量为40. 63%,包封率为54. 03%,106h时累计释放率达到91. 72%,此后基本不再释放,缓释效果良好。
The chitosan,PLGA and azithromycin were used as source materials,the CS/PLGA slow-release composites loading azithromycin were prepared.The properties were tested by specific surface area and pore size analysis( BET),field emission scanning electron microcopy( FESEM),thermogravimetry-mass spectrometry( TG-MS) and ultraviolet-visible spectrophotometry( UV). The results of BET proved that the average pore size of the composites were in the range of 2 ~ 50 nm,which satisfied the requirement of the porous composites; the results of FESEM proved that composites which loading azithromycin loaded successfully.The results of TGMS showed that when temperature came to 500℃,the residual mass of CS/PLGA composites loading azithromycin was 33. 8%,the residual mass of azithromycin was 14. 2%,the thermal properties of the CS/PLGA composites loading azithromycin was better than the azithromycin.The UV experiments determinated that the drug loading of reliever was 40. 63%,envelop rate was 54. 03%. It didn't release when the release rate reached 91. 72%after 106 hours,which proved that the sustained-release effect of this reliever was the best.
The chitosan,PLGA and azithromycin were used as source materials,the CS/PLGA slow-release composites loading azithromycin were prepared.The properties were tested by specific surface area and pore size analysis( BET),field emission scanning electron microcopy( FESEM),thermogravimetry-mass spectrometry( TG-MS) and ultraviolet-visible spectrophotometry( UV). The results of BET proved that the average pore size of the composites were in the range of 2 ~ 50 nm,which satisfied the requirement of the porous composites; the results of FESEM proved that composites which loading azithromycin loaded successfully.The results of TGMS showed that when temperature came to 500℃,the residual mass of CS/PLGA composites loading azithromycin was 33. 8%,the residual mass of azithromycin was 14. 2%,the thermal properties of the CS/PLGA composites loading azithromycin was better than the azithromycin.The UV experiments determinated that the drug loading of reliever was 40. 63%,envelop rate was 54. 03%. It didn't release when the release rate reached 91. 72%after 106 hours,which proved that the sustained-release effect of this reliever was the best.
引文
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[20]周莉,吴凤群,罗仲宽,等.多孔载药纳米羟基磷灰石/聚酰胺/壳聚糖复合材料的制备与性能[J].化学研究与应用,2017,29(1):89-93.
[2]于永鹏,徐孝旭.壳聚糖作为药物缓释载体的应用及进展[J].材料导报,2011,25(S1):384-390.
[3]王明波,冯庆玲,佘振定,等.PLGA微球包埋药物的稳定性及释放研究的新进展[J].功能材料,2011,42(4):591-595.
[4]Rosa G D,Iommelli R,Rotonda M L,et al.Influence of the co-encapsulation of different non-ionic surfactants on the properties Of PLGA insulin-loaded microspheres[J]. J Control Release,2000,69(2):283-295.
[5]何晋浙,姚丽娜,孙培龙.响应面试验优化猴头菌素-PL GA微球制备工艺及其体外释药性能[J].食品科学,2017,38(6):242-247.
[6]马方奎,包子娴,陈西广.壳聚糖聚乳酸羟基乙酸纳米粒子作为抗瘤药物载体的研究[J].中国海洋大学学报(自然科学版),2014,44(07):58-63.
[7]王哲,张秀梅,倪宏哲,等.聚乳酸载阿奇霉素微球包裹和体外释放行为[J].中国生物医学工程学报,2009,28(2):314-316.
[8]韩永涛,黄桂华,席延卫,等.阿奇霉素聚乳酸微球的制备及其体外释药特性的研究[J].山东大学学报(医学版),2006,44(8):853-856.
[9]刘星辰,杨帆.阿奇霉素明胶微球制备工艺研究[J].广东药学院学报,2008,24(2):41-43.
[10]张杰,杨静,甄卫军.聚乳酸/Fe3O4载阿奇霉素微球的制备及其体外释药特性的研究[J].精细石油化工进展,2012,13(1):51-54.
[11]庞锦英,莫羡忠,刘钰馨.阻燃香蕉纤维增强聚乳酸复合材料的制备与表征[J].化工进展,2015,34(4):1050-1 054.
[12]庞锦英,莫羡忠,谭登峰,等.香蕉纤维素微晶/聚乳酸气凝胶及其制备方法和用途[P]. CN:105017541,2015-07-09.
[13]班长伟,牛明军,段瑞侠,等.聚乳酸/壳聚糖复合材料的制备及性能研究[J].包装工程,2015,36(23):72-74,88.
[14]马喜峰,汤春妮,李斌.聚乳酸/纳米四氧化三铁载阿奇霉素缓释剂的制备及性能研究[J].化学与生物工程,2014,31(2):35-37.
[15]黄天辉,郭晓霖.乳液冷冻干燥法制备壳聚糖/聚乳酸复合材料[J].北京航空航天大学学报,2017,43(1):1-5.
[16]刘敬肖,曾淼,史非,等. SIO2气凝胶/壳聚糖复合药物载体材料的制备和表征[J].功能材料,2007,38(9):1 527-1 530.
[17]谢剑飞,李春艳,洪舒寒,等.紫外分光光度计法测定载药纳米纤维毡的药物释放性能[J].中国纤检,2013,18:82-85.
[18]段韶军.阿霉素聚乳酸微球的含量测定[J].山西医药杂志(下半月刊),2010,39(2):163-164.
[19]Mohammadi G,Valizadeh H,Barzegar-Jalali M,et al.Development of azithromycin-PLGA nanoparticles:physicochemical characterization and antibacterial effect against salmonella typhi[J]. Colloid Surface B,2010,80(1):34-39.
[20]周莉,吴凤群,罗仲宽,等.多孔载药纳米羟基磷灰石/聚酰胺/壳聚糖复合材料的制备与性能[J].化学研究与应用,2017,29(1):89-93.