青龙衣膏对胃癌BGC-823细胞侵袭能力的影响
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摘要
目的:探讨青龙衣膏对胃癌BGC-823细胞侵袭能力的影响。方法:采用不同浓度的青龙衣膏处理胃癌BGC-823细胞24、48 h,通过噻唑蓝比色法检测青龙衣膏对细胞生长的影响,并计算半数抑制浓度(IC_(50));以不影响细胞生长的青龙衣膏浓度作为检测其对细胞侵袭能力影响的浓度;将细胞分为对照组、低剂量组(0.66 mg/ml)、中剂量组(0.74 mg/ml)及高剂量组(0.82 mg/ml),处理胃癌24 h;采用噻唑蓝比色法检测细胞与胞外基质(ECM)的黏附能力;Transwell小室法检测细胞迁移和侵袭能力;western blot法检测胃癌BGC-823细胞环氧酶2(COX-2)表达。结果:青龙衣膏可明显抑制胃癌BGC-823细胞增殖,呈现一定的时间-剂量依赖关系。对照组,低、中及高剂量组处理后24 h后的细胞黏附率分别为100%、(88.39±2.51)%、(90.23±7.27)%及(81.37±2.0)%;迁移细胞数分别为(134±23.23)、(27.70±5.16)、(28.70±6.27)及(32.00±3.65)个;侵袭细胞数分别为(95.00±9.23)、(13.00±1.10)、(15.10±2.91)及(27.80±4.04)个;且与对照组比较,低、中及高剂量组的COX-2蛋白表达水平明显降低,差异有极显著统计学意义(P<0.01)。结论:青龙衣膏可抑制胃癌BGC-823细胞的侵袭能力,可能与抑制COX-2的表达相关。
OBJECTIVE: To probe into the effects of Qinglongyi electuary on invasion ability of gastric cancer BGC-823 cells. METHODS: The gastric cancer BGC-823 cells were treated with different concentrations of Qinglongyi electuary for 24 and 48 h. The effects of Qinglongyi electuary on cell growth was detected by MTT assay, and the IC_(50) value was calculated. The concentration of Qinglongyi electuary, which did not affect the cell growth, was used as the dose to test the effects of Qinglongyi electuary on cell invasion ability. The cells were divided into the control group, the low dose group(0.66 mg/ml), the medium dose group(0.74 mg/ml) and the high(0.82 mg/ml) dose group to treat gastric cancer BGC-823 cells for 24 h. Cell adhesion to extracellular matrix(ECM) was determined by MTT assay. Transwell chamber assay was used to detect the ability of cell migration and invasion.Western blot analysis was adopted to detect the cyclooxygenase 2(COX-2) expression in gastric cancer cells. RESULTS: Qinglongyi electuary can significantly inhibit the proliferation of gastric cancer BGC-823 cells, showing a certain time-dose dependence. The cell adhesion rates were 100%,(88.39±2.51)%,(90.23±7.27)% and(81.37±2.0)% respectively in the control group, low, medium and high dose group after treatment of 24 h. The number of cell migration was(134±23.23),(27.70±5.16),(28.70±6.27) and(32.00±3.65), respectively. The number of cells invaded was(95.00±9.23),(13.00±1.10),(15.10±2.91) and(27.80±4.04), respectively. Compared with the control group, the expression levels of COX-2 protein in the low, medium and high dose groups were significantly decreased, with extremely statistically significant differences(P<0.01). CONCLUSIONS: Qinglongyi electuary can inhibit the invasion ability of gastric cancer BGC-823 cells, which may be related to the inhibition of COX-2 expression.
OBJECTIVE: To probe into the effects of Qinglongyi electuary on invasion ability of gastric cancer BGC-823 cells. METHODS: The gastric cancer BGC-823 cells were treated with different concentrations of Qinglongyi electuary for 24 and 48 h. The effects of Qinglongyi electuary on cell growth was detected by MTT assay, and the IC_(50) value was calculated. The concentration of Qinglongyi electuary, which did not affect the cell growth, was used as the dose to test the effects of Qinglongyi electuary on cell invasion ability. The cells were divided into the control group, the low dose group(0.66 mg/ml), the medium dose group(0.74 mg/ml) and the high(0.82 mg/ml) dose group to treat gastric cancer BGC-823 cells for 24 h. Cell adhesion to extracellular matrix(ECM) was determined by MTT assay. Transwell chamber assay was used to detect the ability of cell migration and invasion.Western blot analysis was adopted to detect the cyclooxygenase 2(COX-2) expression in gastric cancer cells. RESULTS: Qinglongyi electuary can significantly inhibit the proliferation of gastric cancer BGC-823 cells, showing a certain time-dose dependence. The cell adhesion rates were 100%,(88.39±2.51)%,(90.23±7.27)% and(81.37±2.0)% respectively in the control group, low, medium and high dose group after treatment of 24 h. The number of cell migration was(134±23.23),(27.70±5.16),(28.70±6.27) and(32.00±3.65), respectively. The number of cells invaded was(95.00±9.23),(13.00±1.10),(15.10±2.91) and(27.80±4.04), respectively. Compared with the control group, the expression levels of COX-2 protein in the low, medium and high dose groups were significantly decreased, with extremely statistically significant differences(P<0.01). CONCLUSIONS: Qinglongyi electuary can inhibit the invasion ability of gastric cancer BGC-823 cells, which may be related to the inhibition of COX-2 expression.
引文
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[15] 郭雪莹,崔红霞,张雅丽,等.五龙消癥丸抑制人胃癌BGC-823细胞侵袭与迁移作用及机制研究[J],中草药,2018,49(16):3788-3796.
[2] Ji YB,Qu ZY,Zou X.Juglone-induced apoptosis in human gastric cancer SGC-7901 cells via the mitochondrial pathway[J].Exp Toxicol Pathol,2011,63(1-2):69-78.
[3] Xu HL,Yu XF,Qu SC,et al.Anti-proliferative effect of Juglone from Juglans mandshurica Maxim on human leukemia cell HL-60 by inducing apoptosis through the mitochondria-dependent pathway[J].Eur J Pharmacol,2010,645(1-3):14-22.
[4] Glogauer JE,Sun CX,Bradley G,et al.Neutrophils Increase Oral Squamous Cell Carcinoma Invasion through an Invadopodia-Depe-ndent Pathway[J].Cancer Immunol Res,2015,3(11):1218-1226.
[5] Ma Y,Luo W,Bunch BL,et al.1,25D3 differentially suppresses bladder cancer cell migration and invasion through the induction of miR-101-3p[J].Oncotarget,2017,8(36):60080-60093.
[6] 季宇彬,曲中原,邹翔,等.青龙衣中胡桃醌对S180肉瘤小鼠的抑瘤作用研究[J].中国药学杂志,2009,44(3):195-199.
[7] 段玉敏,张洪娟,张志华,等.青龙衣胶囊在荷瘤小鼠体内抗肿瘤活性的研究[J].中国实验方剂学杂志,2010,16(9):125-127.
[8] 高启龙,朱亚楠,石变,等.青龙衣含药血清抑制胃癌SGC-7901细胞生长和诱导凋亡[J].中国实验方剂学杂志,2017,23(13):111-117.
[9] 张强,段玉敏,王伟明,等.复方青龙衣胶囊对人胃癌SGC-7901细胞凋亡的形态学影响[J].中国实验方剂学杂志,2012,18(16):181-183.
[10] 陈丽,张建,汪思应,等.胡桃醌对血管形成的影响及其机制初探[J].中国临床药理学与治疗学,2010,15(4):403-410.
[11] 汲晨锋,肖凤,季宇彬.青龙衣多糖对S180小鼠红细胞膜唾液酸水平、ATP酶活性及膜电位的影响[J].中草药,2007,38(11):1685-1687.
[12] 孙海兵,魏永长,涂宏蕾,等.COX-2、PKC-α和miR-101在胃癌中的表达及相关性[J].南方医科大学学报,2013,33(4):559-562.
[13] Echizen K,Hirose O,Maeda Y,et al.Inflammation in gastric cancer:Interplay of the COX-2/prostaglandin E2and Toll-like receptor/MyD88 pathways[J].Cancer Sci,2016,107(4):391-397.
[14] Song L,Guo Y,Xu B.Expressions of Ras Homolog Gene Family,Member A (RhoA) and Cyclooxygenase-2 (COX-2) Proteins in Early Gastric Cancer and Their Role in the Development of Gastric Cancer[J].Med Sci Monit,2017,23:2979-2984.
[15] 郭雪莹,崔红霞,张雅丽,等.五龙消癥丸抑制人胃癌BGC-823细胞侵袭与迁移作用及机制研究[J],中草药,2018,49(16):3788-3796.