贵长猕猴桃多酚抗氧化及抑制亚硝化作用研究
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摘要
对贵长猕猴桃多酚的抗氧化活性及抑制亚硝化作用进行研究。采用超声辅助乙醇浸提的方法提取猕猴桃多酚,以没食子酸为标准物质来测定提取物的多酚含量。通过对DPPH、羟基自由基清除率及总还原力的测定,评价了贵长猕猴桃多酚的抗氧化性。采用紫外分光光度法,测定了贵长猕猴桃多酚的亚硝酸盐清除率和亚硝胺合成阻断率,以评价其对亚硝化反应的抑制能力。结果表明,贵长猕猴桃中多酚含量为(302.87±7.01)mg GAE/100 g FW。贵长猕猴桃多酚具有较强的抗氧化活性。随质量浓度的增大,其对DPPH自由基、羟基自由基的清除能力和还原能力均显著增加,呈现出明显的剂量依赖效应。其对DPPH自由基、羟基自由基清除活性的半数抑制浓度(IC_(50))分别为0.22、0.45 mg/mL。在模拟人体胃酸条件下(pH3.0、温度37℃),贵长猕猴桃多酚能有效清除亚硝酸盐及阻断亚硝胺合成。随着质量浓度增加,清除率和阻断率先增大最后趋于平稳。其IC_(50)分别为0.077、0.082 mg/mL,优于阳性对照Vc,展现出一定的癌症预防功效。贵长猕猴桃多酚具有良好的抗氧化活性及抑制亚硝化反应的作用。
The antioxidant activity and inhibition of nitrosation by polyphenols from Guichang kiwifruit were studied. The content of Guichang kiwifruit polyphenols extracted with ethanol ultrasonically was determined with gallic acid as the standard substance. Three different methods including DPPH radical scavenging, hydroxyl radical scavenging and reducing power were used for evaluating the antioxidant activities of Guichang kiwifruit polyphenols. The rates of scavenging nitrite and blocking nitrosamine were determined by ultraviolet spectrophotometry. The results showed that the content of polyphenols in Guichang kiwifruit was(302.87±7.01)mg GAE/100 g FW. The antioxidant activity assays showed that Guichang kiwifruit polyphenols presented potent antioxidant activity. Moreover, with the concentration increasing, the scavenging rates of DPPH, hydroxyl radical and reducing power increased significantly in a dose-dependent manner. The half inhibitory concentration(IC_(50)) of radical scavenging activity for DPPH and hydroxyl radical were 0.22 mg/mL and 0.45 mg/mL, respectively. In simulated conditions of human gastric juice(pH 3.0, temperature 37 ℃), Guichang kiwifruit polyphenols can effectively eliminate nitrite and block the nitrosamine synthesis. With the concentration increasing, the rates of scavenging and blocking first increased and finally leveled off. The IC_(50) were 0.077 mg/mL and 0.082 mg/mL respectively,better than the positive control Vc, showing a certain cancer prevention effect. The polyphenols from Guichang kiwifruit had strong antioxidant activity and nitrosation inhibition effect.
The antioxidant activity and inhibition of nitrosation by polyphenols from Guichang kiwifruit were studied. The content of Guichang kiwifruit polyphenols extracted with ethanol ultrasonically was determined with gallic acid as the standard substance. Three different methods including DPPH radical scavenging, hydroxyl radical scavenging and reducing power were used for evaluating the antioxidant activities of Guichang kiwifruit polyphenols. The rates of scavenging nitrite and blocking nitrosamine were determined by ultraviolet spectrophotometry. The results showed that the content of polyphenols in Guichang kiwifruit was(302.87±7.01)mg GAE/100 g FW. The antioxidant activity assays showed that Guichang kiwifruit polyphenols presented potent antioxidant activity. Moreover, with the concentration increasing, the scavenging rates of DPPH, hydroxyl radical and reducing power increased significantly in a dose-dependent manner. The half inhibitory concentration(IC_(50)) of radical scavenging activity for DPPH and hydroxyl radical were 0.22 mg/mL and 0.45 mg/mL, respectively. In simulated conditions of human gastric juice(pH 3.0, temperature 37 ℃), Guichang kiwifruit polyphenols can effectively eliminate nitrite and block the nitrosamine synthesis. With the concentration increasing, the rates of scavenging and blocking first increased and finally leveled off. The IC_(50) were 0.077 mg/mL and 0.082 mg/mL respectively,better than the positive control Vc, showing a certain cancer prevention effect. The polyphenols from Guichang kiwifruit had strong antioxidant activity and nitrosation inhibition effect.
引文
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[2]蔡鲁峰,李娜,杜莎,等.N﹣亚硝基化合物的危害及其在体内外合成和抑制的研究进展[J].食品科学,2016,37(5):271﹣277.
[3]邓红,贾洪锋,周世忠,等.果蔬对亚硝酸盐清除作用的研究进展[J].中国调味品,2015,(1):110﹣114.
[4]金方伦,黎明,韩成敏.贵长猕猴桃在黔北地区的生物学特性及丰产优质栽培技术[J].贵州农业科学,2009,37(10):175﹣178.
[5]涂宗财,傅志丰,王辉,等.红薯叶不同溶剂提取物抗氧化性及活性成分鉴定[J].食品科学,2015,36(17):1﹣6.
[6]张艳,段雪芹,高刚,等.响应面法优化姜黄素的提取工艺及其抗氧化活性的研究[J].食品工业科技,2015,36(6):269﹣273.
[7]曾维才,石碧.天然产物抗氧化活性的常见评价方法[J].化工进展,2013,32(6):1205﹣1213.
[8]张声源,庄远杯,罗铝铿,等.红冬蛇菰提取物抗氧化及抑制亚硝化作用研究[J].天然产物研究与开发,2017,(12):2081﹣2086.
[9]王晓波,李金芳,王梅,等.山竹壳总黄酮抗氧化及抑制亚硝化作用研究[J].食品研究与开发,2013,(6):9﹣13.
[10]李琛,张婷,罗安伟,等.8种猕猴桃抗氧化活性评价及基于HPLC与FT﹣IR指纹分析的品种区分[J].现代食品科技,2016,(6):288﹣297.
[11]樊梓鸾,王振宇.红豆越橘体外抗氧化和抗细胞增殖活性研究[J].现代食品科技,2010,26(10):1081﹣1086.
[12]ZUO LL,WANG ZY,FAN ZL,et al.Evaluation of Antioxidant and Antiproliferative Properties of Three Actinidia(Actinidia kolomikta,Actinidia arguta,Actinidia chinensis)Extracts in Vitro[J].International Journal of Molecular Sciences,2012,13(5):5506﹣5518.
[13]刘长江,潘松,梁爽.软枣猕猴桃多糖的体外抗氧化活性[J].食品科学,2012,33(23):79﹣82.
[14]荣建华,李小定,谢笔钧.大豆肽体外抗氧化效果的研究[J].食品科学,2002,23(11):118﹣120.
[15]张婷,李琛,罗安伟,等.8种猕猴桃不同组织部位的体外抗氧化活性[J].食品科学,2016,37(19):88﹣93.
[16]郝教敏,李华强,杨珍平.猕猴桃汁浸提液对肉制品中亚硝酸盐的清除效应[J].食品工业科技,2012,33(7):115﹣118.
[17]宋圃菊,Tannenbaum SR.中华猕猴桃的防癌作用--(一)中华猕猴桃汁阻断N﹣亚硝基吗啉合成[J].营养学报,1984,6(2):109﹣114.
[18]宋圃菊,张琳,丁兰.中华猕猴桃汁的防癌作用--(二)在体外模拟胃液中对亚硝胺合成的阻断作用-Ames试验方法检测[J].营养学报,1984,6(3):241﹣246.
[19]DU GR,LI MJ,MA FW,et al.Antioxidant capacity and the relationship with polyphenol and VC in Actinidia fruits[J].Food Chemistry,2009,113(2):557﹣562.
[20]董彦佐,李学理,何秀丽,等.典型酚类化合物清除亚硝酸钠及对二乙基亚硝胺生成的影响[J].食品科学,2014,35(13):132﹣136.