涤痰汤糖尿病大鼠认知功能障碍的保护作用及机制
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摘要
目的:观察涤痰汤对糖尿病大鼠认知能力的影响,并探讨其对PI3K-AKT信号通路相关因子的影响。方法:将30只SD鼠纳入研究,随机分为正常对照组、模型组及涤痰汤组,其中模型组及涤痰汤组大鼠均接受糖尿病模型制备,涤痰汤组用0. 88 g/m L涤痰汤药液灌胃,正常对照组及模型组用等量的生理盐水灌胃,3组均连续灌胃28 d。各组采用水迷宫评估大鼠学习记忆功能,Tunel检测神经元细胞调亡情况,HE染色法观察海马组织病理结构改变,Western blotting法检测各组海马组织PI3K、AKT、p-AKT、Bax及Bcl-2蛋白水平变化。结果:涤痰汤可明显改善糖尿病模型大鼠的学习记忆能力,抑制神经元凋亡率及Bax水平,增加海马区PI3K、p-AKT、Bcl-2蛋白表达。结论:涤痰汤可明显改善糖尿病模型大鼠的认知,其作用机制可能与介导PI3K/AKT信号通路有关。
Objective: To observe the effects of Ditan decoction on cognitive ability of diabetic rats and to explore the effects of Ditan decoction on PI3 K-AKT signaling pathway related factors. Methods: A total of 30 SD rats were randomly divided into a normal control group,a model group and a Ditan decoction group. t Te rats in the model group and the Ditan decoction group were all prepared by the diabetic model,and the rats in the Ditan decoction group were treated with 0. 88 g/m L Ditan decoction by gavage. The normal control group and the model group were given the same amount of normal saline intragastrically,and the 3 groups were continuously intragastrically administered for 28 days. The learning and memory function of rats in each group was evaluated by water maze,and the apoptosis of neurons was detected by tunnel. The pathological changes of hippocampus were observed by HE staining,and the levels of PI3 K,AKT,p-AKT,Bax and Bcl-2 protein in hippocampus of each group were detected by Western blotting.Results: Ditan decoction could significantly improve the learning and memory ability of diabetic rats,inhibited the neuronal apoptosis rate and the level of Bax,and increased the expression of PI3 K,p-AKT and Bcl-2 protein in hippocampus. Conclusion: Ditan decoction can significantly improve the cognition of diabetic rats,and its mechanism may be related to the mediation of PI3 K/AKT signaling pathway.
Objective: To observe the effects of Ditan decoction on cognitive ability of diabetic rats and to explore the effects of Ditan decoction on PI3 K-AKT signaling pathway related factors. Methods: A total of 30 SD rats were randomly divided into a normal control group,a model group and a Ditan decoction group. t Te rats in the model group and the Ditan decoction group were all prepared by the diabetic model,and the rats in the Ditan decoction group were treated with 0. 88 g/m L Ditan decoction by gavage. The normal control group and the model group were given the same amount of normal saline intragastrically,and the 3 groups were continuously intragastrically administered for 28 days. The learning and memory function of rats in each group was evaluated by water maze,and the apoptosis of neurons was detected by tunnel. The pathological changes of hippocampus were observed by HE staining,and the levels of PI3 K,AKT,p-AKT,Bax and Bcl-2 protein in hippocampus of each group were detected by Western blotting.Results: Ditan decoction could significantly improve the learning and memory ability of diabetic rats,inhibited the neuronal apoptosis rate and the level of Bax,and increased the expression of PI3 K,p-AKT and Bcl-2 protein in hippocampus. Conclusion: Ditan decoction can significantly improve the cognition of diabetic rats,and its mechanism may be related to the mediation of PI3 K/AKT signaling pathway.
引文
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[17]赖文芳,洪海棉,张小琴,等.红景天苷通过激活PI3K/AKT/NRF2通路减轻MCAO大鼠的神经细胞凋亡[J].中华中医药杂志,2016,31(5):1883-1886.
[18]李秀坤,黄英华.基于PI3K/A kt信号转导通路探讨电针足三里、曲池对脑缺血再灌注大鼠神经细胞凋亡的影响[J].重庆医学,2016,45(3):303-306.
[19]Fang Z,Ning J,Xiong C,et al.Effects of Electroacupuncture at Head Points on the Function of Cerebral Motor Areas in Stroke Patients:A PET Study[J].Evid Based Complement Alternat Med,2012,2012:902413.
[2]魏魏,陆媛.糖尿病相关治疗对认知功能影响的研究进展[J].中国全科医学,2019,3(9):1-6.
[3]王飞,罗剑锋,丁玎,等.糖尿病对老年人总体认知功能水平及各认知领域的影响[J].中国临床神经科学,2019,27(1):29-38.
[4]郑静峡.自拟补肾涤痰开窍汤治疗血管性痴呆36例[J].实用中医内科杂志,2005,19(2):135-135.
[5]陈岩.礞石涤痰汤联合西药治疗精神分裂症临床观察[J].光明中医,2018,33(21):3213-3215.
[6]明淑萍.涤痰汤对糖尿病认知功能障碍大鼠干预作用及其机制的研究[D].武汉:湖北中医药大学,2018.
[7]Candeias E,Sebasti2o I,Cardoso S,et al.Brain GLP-1/IGF-1 Signaling and Autophagy Mediate Exendin-4 Protection Against Apoptosis in Type 2Diabetic Rats[J].Mol Neurobiol,2018,55(5):4030-4050.
[8]张毅,张学英,习玲,等.老年2型糖尿病合并轻度认知功能障碍患者的海马磁共振体积及波谱的研究[J].山西医药杂志,2018,47(19):2309-2311.
[9]张红蕊,王耕银,崔昌萌,等.川陈皮素对糖尿病大鼠认知功能障碍的影响[J].中国老年学杂志,2018,38(18):4516-4519.
[10]赵宇昊.在STZ糖尿病大鼠模型中,长非编码RNA H19通过Wnt信号传导通路诱导海马神经元凋亡的相关研究[D].长春:吉林大学,2018.
[11]聂惠琳,姚欣艳.国医大师熊继柏教授从痰论治痫病临床经验[J].湖南中医药大学学报,2018,38(12):1363-1365.
[12]刘飞.桃红四物汤合涤痰汤加减治疗癫痫的疗效[J].临床医药文献(连续型电子期刊),2018,5(86):185.
[13]谢辉.加味涤痰汤治疗缺血性脑卒中后轻度认知障碍100例[J].实用中医药杂志,2018,34(2):174-175.
[14]王耕银.PI3K/AKT/GSK-3β通路在莱菔硫烷调控糖尿病大鼠认知功能减退中的作用[D].石家庄:河北医科大学,2017.
[15]袁有才.新加葛根芩连汤对糖尿病认知障碍大鼠海马微血管损伤及PI3K/AKT/CREB信号通路的作用机制研究[D].成都:成都中医药大学,2015.
[16]陈梦月,陈伟东,柳怡敏,等.紫草素通过PI3K/Akt通路抑制氧糖剥夺诱导的大鼠原代皮层神经元凋亡[J].中国病理生理杂志,2019,35(1):106-111.
[17]赖文芳,洪海棉,张小琴,等.红景天苷通过激活PI3K/AKT/NRF2通路减轻MCAO大鼠的神经细胞凋亡[J].中华中医药杂志,2016,31(5):1883-1886.
[18]李秀坤,黄英华.基于PI3K/A kt信号转导通路探讨电针足三里、曲池对脑缺血再灌注大鼠神经细胞凋亡的影响[J].重庆医学,2016,45(3):303-306.
[19]Fang Z,Ning J,Xiong C,et al.Effects of Electroacupuncture at Head Points on the Function of Cerebral Motor Areas in Stroke Patients:A PET Study[J].Evid Based Complement Alternat Med,2012,2012:902413.