蓖麻二倍体与同源四倍体光合特性和逆境生理指标的比较研究
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摘要
【目的】评价同源四倍体蓖麻的育种意义。【方法】以二倍体和同源四倍体蓖麻为材料,测定光合速率,过氧化物酶(POD)、超氧化物歧化酶(SOD)活性,及叶绿素、可溶性糖、可溶性蛋白、游离脯氨酸(Pro)、丙二醛(MDA)等含量,并比较上述指标的种间差异。【结果】同源四倍体蓖麻的叶绿素含量、净光合速率、叶片可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性均显著地高于二倍体,分别提高30.00%、53.47%、19.23%、25.22%、11.72%、262.07%和24.20%,丙二醛(MDA)的积累明显低于二倍体,降低30.57%。【结论】四倍体蓖麻可为蓖麻种质资源提供较宝贵新种质。
【Objectives】In order to evaluate the breeding significance of autotetraploid castor. 【 Method 】 comparative studies were carried out on photosynthetic characteristics and stress physiological indexes between diploid and autotetraploid in castor in this paper. 【Result】The chlorophyll content,the net photosynthetic rate,the soluble sugar content,the soluble protein content,the free proline content,the peroxidase(POD) and superoxide dismutase(SOD) activity in leaves of the autotetraploid were significantly higher than those in diploid,increased by 30%,53.47%,19.23%,25.22%, 11.72%, 262.07% and 24.20% respectively. Meanwhile the malondiadehyde(MDA) accumulation in autotetraploid was significantly reduced by 30.57%. 【Conclusion】Tetraploid castor provides valuable new germplasm for germplasm resources of castor.
【Objectives】In order to evaluate the breeding significance of autotetraploid castor. 【 Method 】 comparative studies were carried out on photosynthetic characteristics and stress physiological indexes between diploid and autotetraploid in castor in this paper. 【Result】The chlorophyll content,the net photosynthetic rate,the soluble sugar content,the soluble protein content,the free proline content,the peroxidase(POD) and superoxide dismutase(SOD) activity in leaves of the autotetraploid were significantly higher than those in diploid,increased by 30%,53.47%,19.23%,25.22%, 11.72%, 262.07% and 24.20% respectively. Meanwhile the malondiadehyde(MDA) accumulation in autotetraploid was significantly reduced by 30.57%. 【Conclusion】Tetraploid castor provides valuable new germplasm for germplasm resources of castor.
引文
[1]JACKSON R C.Evolution and systematic significance of polyploidy[J].Annu Rev Ecol Syst,1976,7:209-234.
[2]CAVALIER-SMITH T.Nuclear volume control by nucleoskeletal DNA,selection for cell volume and cell growth rate,and the solution of the DNA C-value paradox[J].J Cell Sci,1978,34:247-278.
[3]康向阳.林木多倍体育种研究进展[J].北京林业大学学报,2003,25(4):70-74.
[4]谭德冠,庄南生,黄华孙.林木多倍体诱导的研究进展[J].华南热带农业大学学报,2005,11(1):27-30.
[5]张源源,郭涵,袁红章,等.橡胶树不同倍性的气孔性状差异研究[J].热带作物学报,2017,38(3):389-394.
[6]穆怀志,林琳,于建国,等.Na HCO3胁迫下白桦四倍体表型及生理分析[J].北华大学学报(自然科学版),2016,17(5):585-589.
[7]ZHANG X H,WANG A Y,DU C Q,et al.An efficient method of developing synthetic allopolyploid rice(Oryza spp.)[J].Genet Res Crop Evol,2014,61(4):809-816.
[8]CHANDEL P,TIWARI JK,ALI N,et al.Interspecific potato somatic hybrids between Solanum tuberosum and S.cardiophyllum,potential sources of late blight resistance breeding[J].Plant Cell Tiss Organ Cult,2015,123(3):579-589.
[9]DAMBIER D,BENYAHIA H,PENSABENEBELLAVIA G,et al.Somatic hybridization for citrus rootstock breeding:an effective tool to solve some important issues of the Mediterranean citrus industry[J].Plant Cell Rep,2011,30(5):883-900.
[10]DHOOGHE E,VAN LAERE K,EECKHAUT T,et al.Mitotic chromosome doubling of plant tissues in vitro[J].Plant Cell Tissue Organ Cult,2011,104(3):359-373.
[11]孟凡娟,王秋玉,王建中,等.四倍体刺槐的抗盐性[J].植物生态学报,2008,32(3):654-663.
[12]NIWA Y,SASAKI Y.Plant self-defense mechanisms against oxidative injury and protection of the forest by planting trees of triploids and tetraploids[J].Ecotoxicol Environ Saf,2003,55(1):70-81.
[13]天津南开大学蓖麻工程科技有限公司.四倍体蓖麻的培育方法:CN201510735344.1[P].2016-01-13.
[14]邹琦.植物生理生化实验指导[M].北京:中国农业出版社,2003:19-121.
[15]王健林,关法春.高级作物生理学[M].北京:中国农业大学出版社,2013:20-23.
[16]李成军,王冰林,何启伟,等.日光温室厚皮甜瓜叶片发育进程中生理生化指标的变化[J].中国蔬菜,2007(6):17-20.
[17]XU E K,FAN G Q,NIU S Y,et al.Transcriptomewide profiling and expression analysis of diploid and autotetraploid Paulownia tomentosa×Paulownia fortunei under drought stress[J].PLo S One,2014,9(11):e113313.
[18]颜志明,张蜀宁,汤伟华,等.二倍体和四倍体扬花萝卜的品质特性及抗寒性比较研究[J].西北植物学报,2007,27(7):1405-1410.
[19]王雅美,张蜀宁,郑于莉,等.二、四倍体不结球白菜抗寒基因表达及生理生长特性[J].南京农业大学学报,2014,37(5):41-46.
[20]王园园,尹翠翠,王广东.高温胁迫对二倍体和四倍体杂交兰相关生理生化特征的影响[J].西北植物学报,2013,33(4):735-740.
[21]薛浩,张锋,张志宏,等.‘寒富’苹果与其同源四倍体耐盐差异研究[J].园艺学报,2015,42(5):826-832.
[22]张晓申,翟晓巧,邓敏捷,等.二倍体和四倍体泡桐对干旱胁迫生理响应差异研究[J].河南农业科学,2013,42(11):118-123.
[2]CAVALIER-SMITH T.Nuclear volume control by nucleoskeletal DNA,selection for cell volume and cell growth rate,and the solution of the DNA C-value paradox[J].J Cell Sci,1978,34:247-278.
[3]康向阳.林木多倍体育种研究进展[J].北京林业大学学报,2003,25(4):70-74.
[4]谭德冠,庄南生,黄华孙.林木多倍体诱导的研究进展[J].华南热带农业大学学报,2005,11(1):27-30.
[5]张源源,郭涵,袁红章,等.橡胶树不同倍性的气孔性状差异研究[J].热带作物学报,2017,38(3):389-394.
[6]穆怀志,林琳,于建国,等.Na HCO3胁迫下白桦四倍体表型及生理分析[J].北华大学学报(自然科学版),2016,17(5):585-589.
[7]ZHANG X H,WANG A Y,DU C Q,et al.An efficient method of developing synthetic allopolyploid rice(Oryza spp.)[J].Genet Res Crop Evol,2014,61(4):809-816.
[8]CHANDEL P,TIWARI JK,ALI N,et al.Interspecific potato somatic hybrids between Solanum tuberosum and S.cardiophyllum,potential sources of late blight resistance breeding[J].Plant Cell Tiss Organ Cult,2015,123(3):579-589.
[9]DAMBIER D,BENYAHIA H,PENSABENEBELLAVIA G,et al.Somatic hybridization for citrus rootstock breeding:an effective tool to solve some important issues of the Mediterranean citrus industry[J].Plant Cell Rep,2011,30(5):883-900.
[10]DHOOGHE E,VAN LAERE K,EECKHAUT T,et al.Mitotic chromosome doubling of plant tissues in vitro[J].Plant Cell Tissue Organ Cult,2011,104(3):359-373.
[11]孟凡娟,王秋玉,王建中,等.四倍体刺槐的抗盐性[J].植物生态学报,2008,32(3):654-663.
[12]NIWA Y,SASAKI Y.Plant self-defense mechanisms against oxidative injury and protection of the forest by planting trees of triploids and tetraploids[J].Ecotoxicol Environ Saf,2003,55(1):70-81.
[13]天津南开大学蓖麻工程科技有限公司.四倍体蓖麻的培育方法:CN201510735344.1[P].2016-01-13.
[14]邹琦.植物生理生化实验指导[M].北京:中国农业出版社,2003:19-121.
[15]王健林,关法春.高级作物生理学[M].北京:中国农业大学出版社,2013:20-23.
[16]李成军,王冰林,何启伟,等.日光温室厚皮甜瓜叶片发育进程中生理生化指标的变化[J].中国蔬菜,2007(6):17-20.
[17]XU E K,FAN G Q,NIU S Y,et al.Transcriptomewide profiling and expression analysis of diploid and autotetraploid Paulownia tomentosa×Paulownia fortunei under drought stress[J].PLo S One,2014,9(11):e113313.
[18]颜志明,张蜀宁,汤伟华,等.二倍体和四倍体扬花萝卜的品质特性及抗寒性比较研究[J].西北植物学报,2007,27(7):1405-1410.
[19]王雅美,张蜀宁,郑于莉,等.二、四倍体不结球白菜抗寒基因表达及生理生长特性[J].南京农业大学学报,2014,37(5):41-46.
[20]王园园,尹翠翠,王广东.高温胁迫对二倍体和四倍体杂交兰相关生理生化特征的影响[J].西北植物学报,2013,33(4):735-740.
[21]薛浩,张锋,张志宏,等.‘寒富’苹果与其同源四倍体耐盐差异研究[J].园艺学报,2015,42(5):826-832.
[22]张晓申,翟晓巧,邓敏捷,等.二倍体和四倍体泡桐对干旱胁迫生理响应差异研究[J].河南农业科学,2013,42(11):118-123.