小麦成株期抗条锈病基因YrCH7056的定位及其抗源分析
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摘要
为了更好地利用彭提卡偃麦草资源,拓宽小麦抗源育种选择范围,对其抗条锈性和遗传模式进行探究。利用彭提卡偃麦草渗入系CH7056和小麦品种SY95-71构建重组自交系,以条锈混合菌种CYR32+CYR33+v26对重组自交系的F_7和F_8家系进行成株期抗性鉴定。结果表明:遗传群体家系中抗感单株比例在2013年和2014年间均接近1∶1,由此推断CH7056中携带有1个显性抗条锈病基因,暂命名为YrCH7056。利用细胞学技术(基因组原位杂交)已检测不到外源信号。通过对群体抗感池扫描DArT芯片,将YrCH7056初步定位在小麦1B染色体;之后利用1B染色体上的129对公共SSR标记以及72对新开发的偃麦草特异标记构建了YrCH7056的遗传图谱,侧翼标记为1BL-3848555-1.1c M-YrCH7056-2.5c M-barc240。通过比较抗性表现的时期、基因来源以及紧密连锁标记的遗传距离得出,这个抗条锈病基因不同于已定位于染色体1BL上的抗性基因,推断YrCH7056是一个抗条锈病新基因;同时,1BL-3848555在CH7056中的扩增条带与在彭提卡偃麦草和小偃7430中的条带一致,推断YrCH7056可能来自于彭提卡偃麦草。
Stripe rust resistance and genetic model from Thinopyrum ponticum( 2 n = 10 x = 70) were studied to better utilize the resource and expand the selection range of resistance source for breeding in wheat. Recombinant inbred lines( RILs) were constructed by using CH7056,the introgression line of Th. ponticum,and wheat cultivars SY95-71. The F_7 and F_8 populations from RILs were identified for their adult plant resistance using mixed Pst types CYR32 + CYR33 +v26. The results showed that the proportion of resistant and susceptible individual plants in the genetic groups were both close to 1∶ 1 in 2013 and 2014,suggesting that CH7056 carried one dominant stripe rust resistance gene,temporarily designated YrCH7056. Exogenous signals had not been detected using genomic in situ hybridization. YrCH7056 was located onwheat chromosome 1 B by scaning DArT chip to the bulks of resistance and suspection of RILs. Then 129 pairs of the public SSR markers and 72 pairs of developed Thinopyrum ponticum-specific SSR markers on chromosome 1 B were screened to construct the genetic map of YrCH7056. The flanking markers were 1 BL-3848555 and barc240 with genetic distance of1. 1,2. 5 c M,respectively. The stripe rust resistance gene differs from the resistance gene that had been mapped on chromosome 1 BL by comparing the period of resistance,the source of gene,and the close linkage distance of the marker. It was deduced that YrCH7056 was a new gene of stripe rust resistance. In the meantime,the amplified bands of 1 BL-3848555 among Thinopyrum ponticum,Thinopyrum ponticum-wheat octoploid Xiaoyan 7430 and CH7056 were the same,which showed that YrCH7056 might derive from Thinopyrum ponticum.
Stripe rust resistance and genetic model from Thinopyrum ponticum( 2 n = 10 x = 70) were studied to better utilize the resource and expand the selection range of resistance source for breeding in wheat. Recombinant inbred lines( RILs) were constructed by using CH7056,the introgression line of Th. ponticum,and wheat cultivars SY95-71. The F_7 and F_8 populations from RILs were identified for their adult plant resistance using mixed Pst types CYR32 + CYR33 +v26. The results showed that the proportion of resistant and susceptible individual plants in the genetic groups were both close to 1∶ 1 in 2013 and 2014,suggesting that CH7056 carried one dominant stripe rust resistance gene,temporarily designated YrCH7056. Exogenous signals had not been detected using genomic in situ hybridization. YrCH7056 was located onwheat chromosome 1 B by scaning DArT chip to the bulks of resistance and suspection of RILs. Then 129 pairs of the public SSR markers and 72 pairs of developed Thinopyrum ponticum-specific SSR markers on chromosome 1 B were screened to construct the genetic map of YrCH7056. The flanking markers were 1 BL-3848555 and barc240 with genetic distance of1. 1,2. 5 c M,respectively. The stripe rust resistance gene differs from the resistance gene that had been mapped on chromosome 1 BL by comparing the period of resistance,the source of gene,and the close linkage distance of the marker. It was deduced that YrCH7056 was a new gene of stripe rust resistance. In the meantime,the amplified bands of 1 BL-3848555 among Thinopyrum ponticum,Thinopyrum ponticum-wheat octoploid Xiaoyan 7430 and CH7056 were the same,which showed that YrCH7056 might derive from Thinopyrum ponticum.
引文
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[12]Petya S,Marieta T,Tzveta G,et al.A Modified CTAB method for DNA extraction from soybean and meat products[J].Biotechnol&Biotechnol Eq,2013,27(3):3803-3810.
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[15]刘成,闫红飞,宫文萍,等.小麦叶锈病新抗源筛选[J].植物遗传资源学报,2013,14(5):936-944.
[16]尹静,王广金,张宏纪,等.小麦秆锈抗性遗传及抗性基因研究进展[J].植物遗传资源学报,2007,8(1):106-112.
[17]Qi L,Friebe B,Zhang P,et al.Homoeologous recombination,chromosome engineering and crop improvement[J].Chromosome Res,2007,15:3-19.
[18]Chen X M,Line R F.Inheritance of stripe rust resistance in wheat cultivars postulated to have resistance genes at Yr3 and Yr4 loci[J].Phytopathology,1993,83(4):382-388.
[19]Pahalawatta V,Chen X M.Genetic analysis and molecular mapping of wheat genes conferring resistance to the wheat stripe rust and barley stripe rust pathogens[J].Phytopathology,2005,95(4):427-432.
[20]William M,Singh R P,Huerta-Espino J,et al.Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat[J].Phytopathology,2003,93(2):153-159.
[21]Lin F,Chen X M.Molecular mapping of genes for race-specific overall resistance to stripe rust in wheat cultivar express[J].Theor Appl Genet,2008,116(6):797-806.
[22]孙善澄.小麦与偃麦草远缘杂交的研究[J].华北农学报,1987,11(2):7-12.
[23]康振生,王晓杰,赵杰,等.小麦条锈菌致病性及其变异研究进展[J].中国农业科学,2015,48(17):3439-3453.
[24]张小娟.小麦抗条锈病基因Yr26的精细作图[J].西安:西北农林科技大学,2013.
[25]鲁秀梅,张宁,陈劲枫,等.作物基因聚合育种的研究进展[J].分子植物育种,2017,15(4):1445-1454.
[2]王步云.小麦条锈菌中国鉴别寄主尤皮Ⅱ号抗条锈病基因的精细定位[D].北京:中国农业科学院,2014.
[3]王乐,张晓军,郭慧娟,等.抗条锈病小偃麦渗入系的HMW-GS组成分析[J].山西农业科学,2013,41(10):1027-1030.
[4]侯丽媛,乔麟轶,张晓军,等.抗条锈病基因Yr CH5026的遗传分析及分子定位[J].华北农学报,2015,30(5):7-15.
[5]詹海仙.小偃麦衍生品系鉴定及白粉病和条锈病抗病基因定位[D].西安:电子科技大学,2015.
[6]李欣,畅志坚,詹海仙,等.小麦抗条锈病基因来源及染色体定位的研究进展[J].中国农学通报,2015,31(5):92-95.
[7]Li H J,Wang X M.Thinopyrum ponticum and Th.intermedium:the promising source of resistance to fungal and viral diseases of wheat[J].J Genet Genomics,2009,36(9):557-565.
[8]李振声.小麦远缘杂交新品种——小偃6号[J].山西农业科学,1986(5):30.
[9]殷学贵,尚勋武,庞斌双,等.1A-3中抗条锈新基因Yr Tp1和Yr Tp2的分子标记定位分析[J].中国农业科学,2006,39(1):10-17.
[10]Hou L Y,Jia J Q,Zhang X J,et al.Molecular mapping of the stripe rust resistance gene Yr69 on wheat chromosome 2AS[J].Plant Dis,2016,100(8):1717-1724.
[11]李欣,张晓军,张丛卓,等.兼抗白粉、条锈病小偃麦渗入系CH7124抗性遗传及细胞学鉴定[J].植物遗传资源学报,2012,13(4):577-588.
[12]Petya S,Marieta T,Tzveta G,et al.A Modified CTAB method for DNA extraction from soybean and meat products[J].Biotechnol&Biotechnol Eq,2013,27(3):3803-3810.
[13]Kantarski T,Larson S,Zhang X,et al.Development of the first consensus genetic map of intermediate wheatgrass(Thinopyrum intermedium)using genotyping-by-sequencing[J].Theor Appl Genet,2016,130(1):1-14.
[14]Somers D J,Isaac P,Edwards K.A high-density microsatellite consensus map for bread wheat(Triticum aestivum L.)[J].Theor Appl Genet,2004,109(6):1105-1114.
[15]刘成,闫红飞,宫文萍,等.小麦叶锈病新抗源筛选[J].植物遗传资源学报,2013,14(5):936-944.
[16]尹静,王广金,张宏纪,等.小麦秆锈抗性遗传及抗性基因研究进展[J].植物遗传资源学报,2007,8(1):106-112.
[17]Qi L,Friebe B,Zhang P,et al.Homoeologous recombination,chromosome engineering and crop improvement[J].Chromosome Res,2007,15:3-19.
[18]Chen X M,Line R F.Inheritance of stripe rust resistance in wheat cultivars postulated to have resistance genes at Yr3 and Yr4 loci[J].Phytopathology,1993,83(4):382-388.
[19]Pahalawatta V,Chen X M.Genetic analysis and molecular mapping of wheat genes conferring resistance to the wheat stripe rust and barley stripe rust pathogens[J].Phytopathology,2005,95(4):427-432.
[20]William M,Singh R P,Huerta-Espino J,et al.Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat[J].Phytopathology,2003,93(2):153-159.
[21]Lin F,Chen X M.Molecular mapping of genes for race-specific overall resistance to stripe rust in wheat cultivar express[J].Theor Appl Genet,2008,116(6):797-806.
[22]孙善澄.小麦与偃麦草远缘杂交的研究[J].华北农学报,1987,11(2):7-12.
[23]康振生,王晓杰,赵杰,等.小麦条锈菌致病性及其变异研究进展[J].中国农业科学,2015,48(17):3439-3453.
[24]张小娟.小麦抗条锈病基因Yr26的精细作图[J].西安:西北农林科技大学,2013.
[25]鲁秀梅,张宁,陈劲枫,等.作物基因聚合育种的研究进展[J].分子植物育种,2017,15(4):1445-1454.