跑步运动配合银杏叶提取物摄入对去卵巢肥胖骨质疏松大鼠骨密度、血脂和抗氧化能力影响研究
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摘要
目的探讨跑步运动配合银杏叶提取物摄入对骨密度、骨代谢、血脂和抗氧化能力的影响效果。方法利用80只雌性SD大鼠,随机分为去卵巢高脂组和对照组,去卵巢高脂组进行卵巢切除手术,对照组进行假手术,术后去卵巢高脂组进行高脂饲料喂食,对照组进行正常喂食,共进行6周;将接受切除卵巢手术和高脂喂食的的大鼠又随机分为模型组(M组)、银杏叶组(G组)、运动组(R组)、银杏叶+运动组(GE组),接受假手术和正常喂食的大鼠,为假手术组(S组)。进行12周干预,干预结束后,将大鼠置入代谢笼,采集尿样,后进行大鼠的腹腔静脉取血,对大鼠进行骨扫描、血液生化指标检查、骨代谢标志物检查。结果体重方面,在干预后3、6、9、12周M组大鼠体重显著高于S组,P<0.05,R组、G组、GR组大鼠体重有逐渐上升趋势,但是在3、6、9、12周均显著低于M组,P<0.05。骨密度和骨代谢方面,M组大鼠BMD、血清雌二醇水平显著低于R组、G组GR组、S组大鼠,P<0.05。M组大鼠MDA水平显著高于S组、R组、G组和GR组,P<0.05;M组大鼠血清OC、ALP、尿液DPD/Cre、Ca/Cre、P/Cre显著高于S组、R组、G组和GR组,P<0.05。抗氧化方面,M组大鼠CAT、GSH-PX水平显著低于S组、R组、G组和GR组,P<0.05;血脂方面,M组大鼠血液TG、TC水平显著高于S组、R组、G组和GR组,P<0.05。结论去卵巢肥胖骨质疏松大鼠利用跑步运动配合摄入银杏叶提取物或单纯摄入银杏叶提取物进行中长期干预治疗能够增加骨密度和雌激素水平,降低骨吸收和骨转换率、降低体重和血脂水平、增加抗氧化能力。
Objective To investigate the effects of treadmill exercise with Ginkgo biloba extract on bone mineral density, bone metabolism, blood lipids and antioxidant capacity. Methods Eighty female Sprague-Dawley rats were randomly divided into the ovariectomized high-fat group and the control group, the ovariectomized high-fat group for oophorectomy, the control group for sham operation. For the postoperative ovariectomized high-fat group,high-fat diet was fed, and the control group was fed normally for 6 weeks. Rats undergoing ovariectomy and high-fat feeding were once again randomly divided into model group(M group), Ginkgo biloba group(G group), and exercise group(R group), Ginkgo biloba + exercise group(GE group).The rats undergoing sham surgery and normal feeding were sham-operated groups(S group). After 12 weeks, the rats were placed in metabolic cages and urine samples were collected. Blood samples were taken from the abdominal cavity of rats, and bone density, blood biochemical indicators, and bone metabolism markers were examined. Results In terms of body weight, the body weight of M group was significantly higher than that of S group at 3, 6, 9 and 12 weeks after intervention, P<0.05. The weight of rats in group R, G and GR gradually increased, but at 3, 6, 9, and 12 weeks, they were significantly lower than group M, P<0.05. In terms of bone mineral density and bone metabolism, the BMD and serum estradiol in the M group were significantly lower than those in the R group, the G group, and the S group, P<0.05. The MDA level in group M was significantly higher than that in group S, group R, group G and group GR, P<0.05. The serum OC, ALP, urine DPD/Cre, Ca/Cre and P/Cre in group M were significant higher than S group, R group, G group and GR group, P<0.05. The levels of CAT and GSH-PX in group M were significantly lower than those in group S, group R, group G and group GR, P<0.05. The levels of blood TG and TC in group M were significantly higher than those in group S and R. Group, G group and GR group, P<0.05. Conclusion The bone mineral density and estrogen levels, bone resorption and bone turnover, body weight and blood lipid levels, antioxidant capacity of ovariectomized obese osteoporosis rats can be improved by using running exercise with Ginkgo biloba extract or simply ingesting Ginkgo biloba extract.
Objective To investigate the effects of treadmill exercise with Ginkgo biloba extract on bone mineral density, bone metabolism, blood lipids and antioxidant capacity. Methods Eighty female Sprague-Dawley rats were randomly divided into the ovariectomized high-fat group and the control group, the ovariectomized high-fat group for oophorectomy, the control group for sham operation. For the postoperative ovariectomized high-fat group,high-fat diet was fed, and the control group was fed normally for 6 weeks. Rats undergoing ovariectomy and high-fat feeding were once again randomly divided into model group(M group), Ginkgo biloba group(G group), and exercise group(R group), Ginkgo biloba + exercise group(GE group).The rats undergoing sham surgery and normal feeding were sham-operated groups(S group). After 12 weeks, the rats were placed in metabolic cages and urine samples were collected. Blood samples were taken from the abdominal cavity of rats, and bone density, blood biochemical indicators, and bone metabolism markers were examined. Results In terms of body weight, the body weight of M group was significantly higher than that of S group at 3, 6, 9 and 12 weeks after intervention, P<0.05. The weight of rats in group R, G and GR gradually increased, but at 3, 6, 9, and 12 weeks, they were significantly lower than group M, P<0.05. In terms of bone mineral density and bone metabolism, the BMD and serum estradiol in the M group were significantly lower than those in the R group, the G group, and the S group, P<0.05. The MDA level in group M was significantly higher than that in group S, group R, group G and group GR, P<0.05. The serum OC, ALP, urine DPD/Cre, Ca/Cre and P/Cre in group M were significant higher than S group, R group, G group and GR group, P<0.05. The levels of CAT and GSH-PX in group M were significantly lower than those in group S, group R, group G and group GR, P<0.05. The levels of blood TG and TC in group M were significantly higher than those in group S and R. Group, G group and GR group, P<0.05. Conclusion The bone mineral density and estrogen levels, bone resorption and bone turnover, body weight and blood lipid levels, antioxidant capacity of ovariectomized obese osteoporosis rats can be improved by using running exercise with Ginkgo biloba extract or simply ingesting Ginkgo biloba extract.
引文
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[18] Zhang H, Yip PS. Female sexual dysfunction among young and middle-aged women in Hong Kong. Prevalence and risk factors[J].J Sex Med, 2012, 9(11):2911-2918.
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[20] 程林. 跑步运动结合摄取杜仲提取物对去卵巢大鼠骨密度和抗氧化能力的影响[J].中国组织工程研究,2018,6:3837-3842
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[23] Chobanian AV,Bakris GL,Black HR,et al. The seventh report of the joint national committee on prevention,detection, evaluation,and treatment of high blood pressure: the JNC 7 report[J].JAMA,2003,289(19): 2560-2572.
[2] Greenland P, Fuster V. Cardiovascular risk factor control for all[J]. JAMA, 2017, 318 (2):130-131.
[3] Haghikia A, Gold R. Positive effect on multiple sclerosis with treatment of metabolic syndrome[J]. JAMA Neurol, 2016, 73 (5):499-501.
[4] Nagpal M, De D, Handa S,et al. Insulin resistance and metabolic syndrome in young men with acne[J]. JAMA Dermatol, 2016, 152 (4):399-404.
[5] Lu J,Wang L,Li M,et al. Metabolic syndrome among adults in China - The 2010 China noncommunicable disease surveillance[J]. J Clin Endocrinol Metab, 2017, 102 (2):507-515.
[6] Daudt LD,Musskopf ML, Mendez M,et al. Association between metabolic syndrome and periodontitis: a systematic review and meta-analysis[J]. Brazil Oral Res, 2018, 32 (3):e35.
[7] Lim MY,You HJ,Yoon HS,et al. The effect of heritability and host genetics on the gut microbiota and metabolic syndrome[J]. Gut, 2017, 66 (6):1031-1038.
[8] Seidler,Todd,Fried,et al. A muddied industry: essential risk management strategies for mud run participants, race organizers, and sponsors — part II[J]. Acsm S Health Fitness J, 2014, 18 (5):42-45.
[9] 王伟,李洪军,王得利. 银杏内酯B对缺氧缺血性脑病血小板活化因子的影响[J].中国临床康复,2004,8(27):5886-5887.
[10] Gu Xu.Effect of Ginkgo biloba extract on adipogenic differentiation ability of bone marrow mesenchymal stem cells in rat.J Jilin University (Med Ed),2015,02:343-346, 442.
[11] 张慧彦,秦娇娇,齐晓爽,等,银杏叶提取物不同给药方式对骨质疏松大鼠成骨的影响[J].中国骨质疏松杂志,2016,22(9):1154-1158.
[12] Rachner TD,Khosla S,Hofbauer LC.Osteoporosis: now and the future[J].Lancet,2011,377(9773): 1276-1287.
[13] Cairoli E, Zhukouskaya VV, Eller-Vainicher C,et al.Perspectives on osteoporosis therapies[J].Endocrinol Invest,2015,38(3): 303-311.
[14] Sae Jeong Y,Soon Young H,Sei Hyun B, et al. Serum magnesium level is associated with type 2 diabetes in women witha history of gestational diabetes mellitus: the Korea National Diabetes Program study[J].J Korean Med Sci,2013,29(1): 84-89.
[15] Lokanatha V, Subramanyam D, Rajendra W. Role of free radicals and antioxidants in gynecological cancers: current status and future prospects. [J] Oxidants Antioxidants Med Sci,2014,3(1):15-26.
[16] Mokuda S, Okuda Y, Onishi M, et al. Post-menopausal women with rheumatoid arthritis who are treated with raloxifene or alendronate or glucocorticoids have lower serum undercarboxylated osteocalcin levels [J].J Endocrinol Inv,2012,35(7): 661-664.
[17] Pan JH, Zhang HX, Li FF, et al. The dynamic effects ofAstragalus on bone tissue of ovariectomized rats [J].Chin J Basic Med Tradit Chin Med,2010,16(3): 251-253.
[18] Zhang H, Yip PS. Female sexual dysfunction among young and middle-aged women in Hong Kong. Prevalence and risk factors[J].J Sex Med, 2012, 9(11):2911-2918.
[19] Baber RJ, Panay N, Fenton A.2016 IMS Recommendations onwomen’s midlife health and menopause hormone therapy[J].Climacteric, 2016, 19(2):109-150.
[20] 程林. 跑步运动结合摄取杜仲提取物对去卵巢大鼠骨密度和抗氧化能力的影响[J].中国组织工程研究,2018,6:3837-3842
[21] Hweta B,Rimi S,Sri V M,et al. Antioxidant status,lipid peroxidation and nitric oxide end products in patients of type 2 diabetesmellitus with nephropathy[J].Clin Biochem, 2003,36 (7): 557-561.
[22] Miguel AZ,Jose B,Alex F,et al. Insulin resistance and diabetic macular oedema in type 2 diabetes mellitus[J]. Br Jophthalmol,2010,94(9):1230-1232.
[23] Chobanian AV,Bakris GL,Black HR,et al. The seventh report of the joint national committee on prevention,detection, evaluation,and treatment of high blood pressure: the JNC 7 report[J].JAMA,2003,289(19): 2560-2572.