基于培养基压力控制的组织工程化生物膜动态培养系统的研究
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摘要
目的研究压力大小可控的培养系统,以实现组织工程化生物膜的动态培养。方法根据帕斯卡定律的基本原理,设计培养基压力的控制方法,建立由培养皿、蠕动泵、压力控制模块、主控器与检测模块等组成的组织工程化生物膜动态培养系统。结果该系统可实现培养基于0.12～19.76 mmHg压力范围内的控制。结论该系统为适宜压力范围内的生物膜培养提供所需的压力环境,为组织工程化生物膜动态培养技术提供新思路。
Objective To investigate the culture system that can control the culture pressure and realize the dynamic culture of tissue engineered biofilm. Methods According to the basic principle of Pascal's law, a control method of media pressure is designed, and a tissue engineered biofilm dynamic culture system is established. The system is composed of culture dish, peristaltic pump, pressure control module, main controller and detection module. Results This system can realize the pressure control of the culture with a pressure range of 0.12-19.76 mmHg. Conclusion This system provides the pressure environment of biofilm in suitable pressure range required for culture. It provides a new method for tissue engineered biofilm dynamic culture technology.
Objective To investigate the culture system that can control the culture pressure and realize the dynamic culture of tissue engineered biofilm. Methods According to the basic principle of Pascal's law, a control method of media pressure is designed, and a tissue engineered biofilm dynamic culture system is established. The system is composed of culture dish, peristaltic pump, pressure control module, main controller and detection module. Results This system can realize the pressure control of the culture with a pressure range of 0.12-19.76 mmHg. Conclusion This system provides the pressure environment of biofilm in suitable pressure range required for culture. It provides a new method for tissue engineered biofilm dynamic culture technology.
引文
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[20]张言,刘玉冰,薛彦柏,等.颅内压和颅内温度监护仪研制及实验研究[J].中国医疗器械杂志,2017,41(2):107-109.
[2]Tsai RJ,Li LM,Chen JK.Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells[J].N Eng JMed,2000,343(2):86-93.
[3]Fan T,Zhao J,Ma X,et al.Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane[J].Mol Vis,2011,17(54-55):469-480.
[4]王丹,张秋华,李彦,等.兔眼角膜成纤维细胞原代培养[J].辽宁中医药大学学报,2012,14(4):142-143.
[5]Rouabhia M,Allaire P.Gingival mucosa regeneration in athymic mice using in vitro engineered human oralmucosa[J].Biomaterials,2010,31(22):5798-5804.
[6]Fukahori M,Chitose S,Sato K,et al.Regeneration of vocalfold mucosa using tissue-engineered structures with oral mucosal cells[J].PLoS One,2016,11(1):e0146-e0151.
[7]苏琰,张长青,曾炳芳.小肠粘膜下层修复组织缺损的应用研究[J].国外医学骨学科分册,2004,25(1):43-45.
[8]李颖杰.细胞层组织工程模拟构建颞下颌关节滑膜的实验研究[D].武汉:武汉大学,2014.
[9]王丽平,刘琴,陈芳,等.佐剂性关节炎模型兔成纤维样滑膜细胞的培养[J].中国组织工程研究,2017,21(12):1872-1876.
[10]孙贵才,徐轶尔,谢晶日,等.四种不同处理方法体外培养大鼠滑膜细胞[J].中国组织工程研究,2012,16(7):1193-1196.
[11]张春秋,李可,高丽兰,等.软骨组织工程构建中的生物力学[J].力学进展,2018,48:410-437.
[12]刁颖敏.组织工程瓣的拉伸试验及力学参数的选取[J].力学季刊,2004,25(1):140-144.
[13]李晓娜,王晓君,贺瑞,等.力学刺激对角膜成纤维细胞碱性成纤维细胞生长因子表达的影响[J].医用生物力学,2012,27(1):72-76.
[14]陆晓娜,徐奕昊,王欢,等.一种可提供循环可控的动态力学刺激的组织工程动态培养仪[J/CD].中华损伤与修复杂志(电子版),2017,12(6):421-424.
[15]陶蒙.组织工程角膜动态培养及透明度检测仪器开发与实验论证[D].杭州:杭州电子科技大学,2015.
[16]曹连民,王忠涛,黄利民,等.基于帕斯卡原理的支架初撑力增压装置[J].煤矿安全,2017,48(1):109-112.
[17]Thuret G,Carricajo A,Chiquet C,et al.Optimizing microbiological controls of corneal organ culture media[J].J Fr Ophtalmol,2003,26(8):792-800.
[18]Schonfelder J,Valtink M,Knels L,et al.Quality assessment of corneal storage media and their components[J].Graefes Arch Clin Exp Ophthalmol,2014,252(1):77-82.
[19]徐渊,赵炜,杜红俊,等.正常视力人群眼压和角膜中央厚度的关系[J].国际眼科杂志,2007,7(4):1034-1035.
[20]张言,刘玉冰,薛彦柏,等.颅内压和颅内温度监护仪研制及实验研究[J].中国医疗器械杂志,2017,41(2):107-109.