深圳大鹏湾六种珊瑚共附生真菌多样性研究
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摘要
本研究使用6种不同培养基对深圳大鹏湾6种珊瑚上的共附生真菌进行平板涂布法分离培养,结合ITS-rDNA基因序列特征和形态学特征进行鉴定来研究深圳大鹏湾海域珊瑚共附生真菌的多样性。共分离培养出457株真菌菌株,分属于7个属,分别为红酵母属Rhodotorula(180株)、曲霉属Aspergillus(170株)、木霉属Trichoderma(50株)、青霉属Penicillium(34株)、枝孢属Cladosporium(21株)、透孢黑团壳属Massarina(1株)和弯颈霉属Tolypocladium(1株),其中优势属为红酵母属Rhodotorula和曲霉属Aspergillus,占菌株分离总数的76.59%。单独鹿角珊瑚上分离到的共附生真菌数量和种类最多,为160株9种;在该种珊瑚上还分离出弯颈霉属Tolypocladium sp.,该属真菌此前从未在珊瑚共附生真菌的研究中分离出来。本研究还发现SDA培养基分离出的共附生真菌数量最多,CDA培养基的真菌数量最少,表明不同培养基分离的共附生真菌多样性存在差异。
Six different kinds of culture media are used to isolate and cultivate the fungi associated with six species of coral collected from Dapeng Bay of Shenzhen. The identification was made through morphological observation in combination with ITS-rDNA blast homology analysis. A total of 457 fungal isolates were recovered and identified, belonging to seven genera including Rhodotorula(180 isolates), Aspergillus(170 isolates), Trichoderma(50 isolates), Penicillium(34 isolates), Cladosporium(21 isolates), Massarina(1 isolate),and Tolypocladium(1 isolate). Among them, Rhodotorula and Aspergillus were dominant, accounting for76.59% of the total number of isolates. The most species of fungi were isolated from Acropora solitaryensis,amounting to nine species(160 isolates). Tolypocladium sp. isolated from Acropora solitaryensis, has not been found before in corals. The most isolates were obtained from SDA medium, while only a few from CDA,indicating that the preference of fungi for culture media was different.
Six different kinds of culture media are used to isolate and cultivate the fungi associated with six species of coral collected from Dapeng Bay of Shenzhen. The identification was made through morphological observation in combination with ITS-rDNA blast homology analysis. A total of 457 fungal isolates were recovered and identified, belonging to seven genera including Rhodotorula(180 isolates), Aspergillus(170 isolates), Trichoderma(50 isolates), Penicillium(34 isolates), Cladosporium(21 isolates), Massarina(1 isolate),and Tolypocladium(1 isolate). Among them, Rhodotorula and Aspergillus were dominant, accounting for76.59% of the total number of isolates. The most species of fungi were isolated from Acropora solitaryensis,amounting to nine species(160 isolates). Tolypocladium sp. isolated from Acropora solitaryensis, has not been found before in corals. The most isolates were obtained from SDA medium, while only a few from CDA,indicating that the preference of fungi for culture media was different.
引文
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Domart-Coulon IJ,Sinclair CS,Hill RT,TambuttéS,Puverel S,Ostrander GK,2004.A basidiomycete isolated from the skeleton of Pocillopora damicornis(Scleractinia)selectively stimulates short-term survival of coral skeletogenic cells.Marine Biology,144(3):583-592
Du XN,Gao YH,Zhang Y,Xu W,Luo ZH,2019.Diversity and antibacterial activities of fungi associated with the South Chinese Sea sponge.Mycosystema,38(1):64-73(in Chinese)
Dunlap WC,Shick JM,1998.Ultraviolet radiation-absorbing mycosporine-like amino acids in coral reef organisms:a biochemical and environmental perspective.Journal of Phycology,34(3):418-430
Guo SS,Xu W,Alias SA,He GY,Luo ZH,2018.Diversity of cultivable fungi associated with four coral species from the South China Sea.Journal of Applied Oceanography,37(1):35-44(in Chinese)
Hayakawa Y,Hattori Y,Kawasaki T,Kanoh K,Adachi K,Shizuri Y,2008.Efrapeptin J,a new down-regulator of the molecular chaperone GRP78 from a marine Tolypocladium sp.The Journal of Antibiotics,61(6):365-371
Kushmaro A,Loya Y,Fine M,1996.Bacterial infection and coral bleaching.Nature,380:396
Le Campion-Alsumard T,Golubic S,Priess K,1995.Fungi in corals:symbiosis or disease?interaction between polyps and fungi causes pearl-like skeleton biomineralization.Marine Ecology Progress Series,117:137-147
Mouchka ME,Hewson I,Harvell CD,2010.Coral-associated bacterial assemblages:current knowledge and the potential for climate-driven impacts.Integrative and Comparative Biology,50(4):662-674
Raghukumar C,Ravindran J,2012.Fungi and their role in corals and coral reef ecosystems.Biology of Marine Fungi,Springer,Berlin,Heidelberg.89-113
Roder C,Arif C,Bayer T,Aranda M,Daniels C,Shibl A,Chavanich S,Voolstra CR,2014.Bacterial profiling of white plague disease in a comparative coral species framework.The ISME Journal,8(1):31-39
Teng XC,Zhuang YB,Wang Y,Liu PP,Xu ZH,Zhu WM,2010.Secondary metabolites from Penicillium sp.gxwz406symbiotic with the gorgonian Echinogorgia flora.Chinese Journal of Marine Drugs,29(4):11-13(in Chinese)
Toledo-Hernández C,Bones-González A,Ortiz-Vázquez OE,Sabat AM,Bayman P,2007.Fungi in the sea fan Gorgonia ventalina:diversity and sampling strategies.Coral Reefs,26(3):725-730
Wang YN,Shao CL,Zheng CJ,Chen YY,Wang CY,2011.Diversity and antibacterial activities of fungi derived from the gorgonian Echinogorgia rebekka from the South China Sea.Marine Drugs,9(8):1379-1390
Wegley L,Edwards R,Rodriguez-Brito B,Liu H,Rohwer F,2007.Metagenomic analysis of the microbial community associated with the coral Porites astreoides.Environmental Microbiology,9(11):2707-2719
Xiao SL,2012.Study on diversity of fungal community associated with three species of corals in Xuwen and antimicrobial activities of the fungi.Master Thesis,Guangdong Ocean University,Zhanjiang.1-67(in Chinese)
Zhang YJ,Zhang S,Liu XZ,Wen HA,Wang M,2010.A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains.Letters in Applied Microbiology,51(1):114-118
Zhang XY,Bao J,Wang GH,He F,Xu XY,Qi SH,2012.Diversity and antimicrobial activity of culturable fungi isolated from six species of the South China Sea gorgonians.Microbial Ecology,64(3):617-627
Zhang XY,Sun YL,Bao J,He F,Xu XY,Qi SH,2012.Phylogenetic survey and antimicrobial activity of culturable microorganisms associated with the South China Sea black coral Antipathes dichotoma.FEMSMicrobiology Letters,336(2):122-130
Zhou J,Jin H,Cai ZH,2014.A review of the role and function of microbes in coral reef ecosystem.Chinese Journal of Applied Ecology,25(3):919-930(in Chinese)
杜晓娜,高渊皓,张雁,徐炜,骆祝华,2019.一株南海指海绵共附生真菌多样性及其抗菌活性.菌物学报,38(1):64-73
郭双双,徐炜,Alias Siti Aisyah,何高阳,骆祝华,2018.南海4种珊瑚附生可培养真菌多样性分析.应用海洋学学报,37(1):35-44
滕宪存,庄以彬,王乂,刘培培,徐志红,朱伟明,2010.花刺柳珊瑚共生真菌Penicillium sp.的次生代谢产物研究.中国海洋药物杂志,29(4):11-13
肖胜蓝,2012.徐闻3种石珊瑚附生可培养真菌多样性及其抗菌活性的研究.广东海洋大学硕士论文,湛江.1-67
周进,晋慧,蔡中华,2014.微生物在珊瑚礁生态系统中的作用与功能.应用生态学报,25(3):919-930
Amend,AS,Barshis DJ,Oliver TA,2012.Coral-associated marine fungi form novel lineages and heterogeneous assemblages.The ISME Journal,6:1291-1301
Balser TC,McMahon KD,Bart D,Bronson D,Coyle DR,Craig N,Flores-Mangual ML,Forshay K,Jones SE,Kent AE,Shade AL,2006.Bridging the gap between micro-and macro-scale perspectives on the role of microbial communities in global change ecology.Plant Soil,289:59-70
Ben-Haim Y,Zicherman-Keren M,Rosenberg E,2003.Temperature-regulated bleaching and lysis of the coral Pocillopora damicornis by the novel pathogen Vibrio coralliilyticus.Applied and Environmental Microbiology,69(7):4236-4242
Domart-Coulon IJ,Sinclair CS,Hill RT,TambuttéS,Puverel S,Ostrander GK,2004.A basidiomycete isolated from the skeleton of Pocillopora damicornis(Scleractinia)selectively stimulates short-term survival of coral skeletogenic cells.Marine Biology,144(3):583-592
Du XN,Gao YH,Zhang Y,Xu W,Luo ZH,2019.Diversity and antibacterial activities of fungi associated with the South Chinese Sea sponge.Mycosystema,38(1):64-73(in Chinese)
Dunlap WC,Shick JM,1998.Ultraviolet radiation-absorbing mycosporine-like amino acids in coral reef organisms:a biochemical and environmental perspective.Journal of Phycology,34(3):418-430
Guo SS,Xu W,Alias SA,He GY,Luo ZH,2018.Diversity of cultivable fungi associated with four coral species from the South China Sea.Journal of Applied Oceanography,37(1):35-44(in Chinese)
Hayakawa Y,Hattori Y,Kawasaki T,Kanoh K,Adachi K,Shizuri Y,2008.Efrapeptin J,a new down-regulator of the molecular chaperone GRP78 from a marine Tolypocladium sp.The Journal of Antibiotics,61(6):365-371
Kushmaro A,Loya Y,Fine M,1996.Bacterial infection and coral bleaching.Nature,380:396
Le Campion-Alsumard T,Golubic S,Priess K,1995.Fungi in corals:symbiosis or disease?interaction between polyps and fungi causes pearl-like skeleton biomineralization.Marine Ecology Progress Series,117:137-147
Mouchka ME,Hewson I,Harvell CD,2010.Coral-associated bacterial assemblages:current knowledge and the potential for climate-driven impacts.Integrative and Comparative Biology,50(4):662-674
Raghukumar C,Ravindran J,2012.Fungi and their role in corals and coral reef ecosystems.Biology of Marine Fungi,Springer,Berlin,Heidelberg.89-113
Roder C,Arif C,Bayer T,Aranda M,Daniels C,Shibl A,Chavanich S,Voolstra CR,2014.Bacterial profiling of white plague disease in a comparative coral species framework.The ISME Journal,8(1):31-39
Teng XC,Zhuang YB,Wang Y,Liu PP,Xu ZH,Zhu WM,2010.Secondary metabolites from Penicillium sp.gxwz406symbiotic with the gorgonian Echinogorgia flora.Chinese Journal of Marine Drugs,29(4):11-13(in Chinese)
Toledo-Hernández C,Bones-González A,Ortiz-Vázquez OE,Sabat AM,Bayman P,2007.Fungi in the sea fan Gorgonia ventalina:diversity and sampling strategies.Coral Reefs,26(3):725-730
Wang YN,Shao CL,Zheng CJ,Chen YY,Wang CY,2011.Diversity and antibacterial activities of fungi derived from the gorgonian Echinogorgia rebekka from the South China Sea.Marine Drugs,9(8):1379-1390
Wegley L,Edwards R,Rodriguez-Brito B,Liu H,Rohwer F,2007.Metagenomic analysis of the microbial community associated with the coral Porites astreoides.Environmental Microbiology,9(11):2707-2719
Xiao SL,2012.Study on diversity of fungal community associated with three species of corals in Xuwen and antimicrobial activities of the fungi.Master Thesis,Guangdong Ocean University,Zhanjiang.1-67(in Chinese)
Zhang YJ,Zhang S,Liu XZ,Wen HA,Wang M,2010.A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains.Letters in Applied Microbiology,51(1):114-118
Zhang XY,Bao J,Wang GH,He F,Xu XY,Qi SH,2012.Diversity and antimicrobial activity of culturable fungi isolated from six species of the South China Sea gorgonians.Microbial Ecology,64(3):617-627
Zhang XY,Sun YL,Bao J,He F,Xu XY,Qi SH,2012.Phylogenetic survey and antimicrobial activity of culturable microorganisms associated with the South China Sea black coral Antipathes dichotoma.FEMSMicrobiology Letters,336(2):122-130
Zhou J,Jin H,Cai ZH,2014.A review of the role and function of microbes in coral reef ecosystem.Chinese Journal of Applied Ecology,25(3):919-930(in Chinese)
杜晓娜,高渊皓,张雁,徐炜,骆祝华,2019.一株南海指海绵共附生真菌多样性及其抗菌活性.菌物学报,38(1):64-73
郭双双,徐炜,Alias Siti Aisyah,何高阳,骆祝华,2018.南海4种珊瑚附生可培养真菌多样性分析.应用海洋学学报,37(1):35-44
滕宪存,庄以彬,王乂,刘培培,徐志红,朱伟明,2010.花刺柳珊瑚共生真菌Penicillium sp.的次生代谢产物研究.中国海洋药物杂志,29(4):11-13
肖胜蓝,2012.徐闻3种石珊瑚附生可培养真菌多样性及其抗菌活性的研究.广东海洋大学硕士论文,湛江.1-67
周进,晋慧,蔡中华,2014.微生物在珊瑚礁生态系统中的作用与功能.应用生态学报,25(3):919-930