基于UPLC-Q-TOF/MS~E快速分析绵茵陈中化学成分
|
摘要
目的应用超高效液相色谱-四级杆飞行时间质谱(UPLC-Q-TOF/MS~E)对绵茵陈提取物中化学成分进行分析。方法以Acquity BEH C_(18)(2.1 mm×100 mm,1.7μm)为色谱柱,乙腈和0.1%甲酸为流动相,梯度洗脱,流速为0.3 mL·min~(-1),采用ESI电喷雾源正离子模式检测。通过自建数据库与UNIFI自带数据库结合检索得到初筛化合物,人工复核各化合物的相对保留时间、分子质量、特征碎片等信息,并结合对照品信息和文献信息对其进行验证。结果共鉴定出41个化合物,包括13个黄酮类、6个香豆素类、11个有机酸类和11个其他类化合物。其中9个化合物为在绵茵陈中首次发现。结论对绵茵陈中的化学成分进行了快速的分析,为绵茵陈的质量控制和药效物质基础研究提供了参考。
Objective To identify the chemical components of capillary wormwood herb extracts by UPLC-QTOF/MS~E. Methods Acquity BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) was used for the chromatographic separation. Gradient elution was carried out with acetonitrile and 0.1% formic acid solution as the mobile phase. The flow rate was 0.3 mL·min~(-1). The detector was ESI ion source, and tested in positive mode.Preliminary screening compounds were obtained by combining self-built database with UNIFI self-contained database. The identified constituents were further confirmed based on accurate mass, fragment ions, retention behaviors, reference standards and so on. Results Totally 41 components were rapidly identified, including13 flavonoids, 6 lignans, 11 phenolic acids, and 11 other compounds. Among them, 9 components were first reported in capillary wormwood herb. Conclusion The chemical components of capillary wormwood herb are studied, which provides a reference for the quality control of capillary wormwood herb and basic research of pharmacodynamic substances.
Objective To identify the chemical components of capillary wormwood herb extracts by UPLC-QTOF/MS~E. Methods Acquity BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) was used for the chromatographic separation. Gradient elution was carried out with acetonitrile and 0.1% formic acid solution as the mobile phase. The flow rate was 0.3 mL·min~(-1). The detector was ESI ion source, and tested in positive mode.Preliminary screening compounds were obtained by combining self-built database with UNIFI self-contained database. The identified constituents were further confirmed based on accurate mass, fragment ions, retention behaviors, reference standards and so on. Results Totally 41 components were rapidly identified, including13 flavonoids, 6 lignans, 11 phenolic acids, and 11 other compounds. Among them, 9 components were first reported in capillary wormwood herb. Conclusion The chemical components of capillary wormwood herb are studied, which provides a reference for the quality control of capillary wormwood herb and basic research of pharmacodynamic substances.
引文
[1]中国药典2015年版.一部[S].2015:239-240.
[2]曹锦花.茵陈的化学成分和药理作用研究进展[J].沈阳药科大学学报,2013,30(6):489-494.
[3]董岩,王新芳,崔长军,等.茵陈蒿的化学成分和药理作用研究进展[J].时珍国医国药,2008,19(4):874-876.
[4]Zhang F,Li M,Qiao L,et al.Rapid characterization of Ziziphi Spinosae Semen by UPLC/Qt of MS with novel informatics platform and its application in evaluation of two seeds from Ziziphus species[J].J Pharm Biomed Anal,2016,122:59-80.
[5]欧阳文竹,尚展鹏,王文建,等.茵陈提取物中化学成分的UHPLC-LTQ-Orbitrap快速鉴定[J].中国中药杂志,2017,42(3):523-530.
[6]邹瑾,周金彩,龙红萍,等.UPLC-ESI-Q-TOF-MS法分析兖州卷柏化学成分[J].中成药,2018,40(10):2334-2337.
[7]李颖,吴茵,李倩,等.水飞蓟素胶囊化学成分的UP-LC-Q-TOF-MS快速鉴定与分析[J].中国医院药学杂志,2017,37(22):2259-2265.
[8]尹智慧,孙长海,方洪壮.槲皮素与桑色素ESI-MS裂解行为的比较分析[J].分析测试学报,2017,36(2):205-211.
[9]元超,王鸿发,胡璇,等.UPLC-Q-TOF-MS~E技术快速定性艾纳香抗菌有效部位的化学成分[J].天然产物研究与开发,2018,30(11):1904-1912.
[10]李泮霖,黄昕,云莎,等.基于UFLC-Triple TOF-MS/MS技术的银杏叶提取物注射液化学物质基础研究[J].中南药学,2017,15(5):573-579.
[11]叶晓珂,秦沛,李伟,等.液相色谱-串联质谱分离鉴定树莓叶中黄酮类化合物[J].质谱学报,2011,32(5):271-277.
[12]郑成凤,潘裕添,蔡文燕,等.HPLC-UV-MS/MS法对金线莲中黄酮类组分的鉴定和测定[J].天然产物研究与开发,2013,25(10):1381-1386.
[13]王志伟,谭晓杰,马婷婷,等.茵陈化学成分的分离与鉴定[J].沈阳药科大学学报,2008,25(10):781-784.
[14]Tan X,Li Q,Chen X,et al.Simultaneous determination of 13 bioactive compounds in Herba Artemisiae Scopariae(Yin Chen)from different harvest seasons by HPLC-DAD[J].J Pharm Biomed Anal,2008,47(4-5):847-853.
[15]Wang X,Sun W,Sun H,et al.Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q-TOF-MS/MS[J].J Pharm Biomed Anal,2008,46(3):477-490.
[16]高添,杨凌鉴,盛鑫康,等.基于HPLC-Q-TOF/MS和GC/MS的香丹注射液化学成分分析[J].中成药,2019,41(2):345-352.
[17]谭金桃,马丹凤,方艳,等.基于UPLC-ESIQ-TOF-MS法分析灯台叶颗粒的主要活性成分[J].中南药学,2017,15(8):1111-1114.
[18]Clifford MN,Johnston KL,Knight S,et al.Hierarchical scheme for LC-MSn identification of chlorogenic acids[J].JAgric Food Chem,2003,51(10):2900-2911.
[19]马宏宇,孙奕,吕阿丽,等.茵陈蒿化学成分的分离与鉴定[J].中国药物化学杂志,2010,20(1):61-63.
[20]Juteau F,Masotti V,Bessiere JM,et al.Compositional characteristics of the essential oil of Artemisia campestris var.glutinosa[J].Biochem Syst Ecol,2002,30(11):1065-1070.
[2]曹锦花.茵陈的化学成分和药理作用研究进展[J].沈阳药科大学学报,2013,30(6):489-494.
[3]董岩,王新芳,崔长军,等.茵陈蒿的化学成分和药理作用研究进展[J].时珍国医国药,2008,19(4):874-876.
[4]Zhang F,Li M,Qiao L,et al.Rapid characterization of Ziziphi Spinosae Semen by UPLC/Qt of MS with novel informatics platform and its application in evaluation of two seeds from Ziziphus species[J].J Pharm Biomed Anal,2016,122:59-80.
[5]欧阳文竹,尚展鹏,王文建,等.茵陈提取物中化学成分的UHPLC-LTQ-Orbitrap快速鉴定[J].中国中药杂志,2017,42(3):523-530.
[6]邹瑾,周金彩,龙红萍,等.UPLC-ESI-Q-TOF-MS法分析兖州卷柏化学成分[J].中成药,2018,40(10):2334-2337.
[7]李颖,吴茵,李倩,等.水飞蓟素胶囊化学成分的UP-LC-Q-TOF-MS快速鉴定与分析[J].中国医院药学杂志,2017,37(22):2259-2265.
[8]尹智慧,孙长海,方洪壮.槲皮素与桑色素ESI-MS裂解行为的比较分析[J].分析测试学报,2017,36(2):205-211.
[9]元超,王鸿发,胡璇,等.UPLC-Q-TOF-MS~E技术快速定性艾纳香抗菌有效部位的化学成分[J].天然产物研究与开发,2018,30(11):1904-1912.
[10]李泮霖,黄昕,云莎,等.基于UFLC-Triple TOF-MS/MS技术的银杏叶提取物注射液化学物质基础研究[J].中南药学,2017,15(5):573-579.
[11]叶晓珂,秦沛,李伟,等.液相色谱-串联质谱分离鉴定树莓叶中黄酮类化合物[J].质谱学报,2011,32(5):271-277.
[12]郑成凤,潘裕添,蔡文燕,等.HPLC-UV-MS/MS法对金线莲中黄酮类组分的鉴定和测定[J].天然产物研究与开发,2013,25(10):1381-1386.
[13]王志伟,谭晓杰,马婷婷,等.茵陈化学成分的分离与鉴定[J].沈阳药科大学学报,2008,25(10):781-784.
[14]Tan X,Li Q,Chen X,et al.Simultaneous determination of 13 bioactive compounds in Herba Artemisiae Scopariae(Yin Chen)from different harvest seasons by HPLC-DAD[J].J Pharm Biomed Anal,2008,47(4-5):847-853.
[15]Wang X,Sun W,Sun H,et al.Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q-TOF-MS/MS[J].J Pharm Biomed Anal,2008,46(3):477-490.
[16]高添,杨凌鉴,盛鑫康,等.基于HPLC-Q-TOF/MS和GC/MS的香丹注射液化学成分分析[J].中成药,2019,41(2):345-352.
[17]谭金桃,马丹凤,方艳,等.基于UPLC-ESIQ-TOF-MS法分析灯台叶颗粒的主要活性成分[J].中南药学,2017,15(8):1111-1114.
[18]Clifford MN,Johnston KL,Knight S,et al.Hierarchical scheme for LC-MSn identification of chlorogenic acids[J].JAgric Food Chem,2003,51(10):2900-2911.
[19]马宏宇,孙奕,吕阿丽,等.茵陈蒿化学成分的分离与鉴定[J].中国药物化学杂志,2010,20(1):61-63.
[20]Juteau F,Masotti V,Bessiere JM,et al.Compositional characteristics of the essential oil of Artemisia campestris var.glutinosa[J].Biochem Syst Ecol,2002,30(11):1065-1070.