新疆石榴皮HPLC指纹图谱研究
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摘要
目的建立新疆石榴皮HPLC指纹图谱。方法采用Agilent SB-C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%三氟乙酸溶液,梯度洗脱,流速1.0 m L/min,柱温30℃,检测波长370 nm,进样量10μL,测定12批石榴皮样品,运用相似度分析软件建立石榴皮指纹图谱共有模式,并对色谱峰进行指认。结果建立了石榴皮HPLC指纹图谱,标定了18个共有峰,3个色谱峰得到指认,分别为α-安石榴苷、β-安石榴苷、鞣花酸。结论本研究建立的石榴皮HPLC指纹图谱方法稳定可行,可用于控制石榴皮质量。
Objective To establish HPLC fingerprints of Granati Pericarpium. Methods The Agilent SB-C18 column(4.6 mm × 250 mm, 5 μm) was adopted with gradient elution by mixture of methanol-water(0.1% trifluoroacetic acid) as the mobile phase. The detection wavelength was 370 nm; the flow rate was 1.0 m L/min; the column temperature was 30 ℃; the sample size was 10 μL. 12 batches of Granati Pericarpium were determined, and the common model was established by using the similarity analysis software. Chromatographic peaks were identified. Results The HPLC fingerprints of Granati Pericarpium was established and 3 out of the 18 common peaks in the fingerprint were identified, which were α-punicalagin, β-punicalagin, ellagic acid. Conclusion The established HPLC fingerprint method of Granati Pericarpium is stable and feasible, which can be used for the quality control of Granati Pericarpium.
Objective To establish HPLC fingerprints of Granati Pericarpium. Methods The Agilent SB-C18 column(4.6 mm × 250 mm, 5 μm) was adopted with gradient elution by mixture of methanol-water(0.1% trifluoroacetic acid) as the mobile phase. The detection wavelength was 370 nm; the flow rate was 1.0 m L/min; the column temperature was 30 ℃; the sample size was 10 μL. 12 batches of Granati Pericarpium were determined, and the common model was established by using the similarity analysis software. Chromatographic peaks were identified. Results The HPLC fingerprints of Granati Pericarpium was established and 3 out of the 18 common peaks in the fingerprint were identified, which were α-punicalagin, β-punicalagin, ellagic acid. Conclusion The established HPLC fingerprint method of Granati Pericarpium is stable and feasible, which can be used for the quality control of Granati Pericarpium.
引文
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[7]JIMENEZ D R M,RAMAZANOV A,SIKORSKI S,et al.A new method of standartization of health-promoting pomegranate fruit(Punica granatum)extract[J].J Georgian Med News,2006,140:70-77.
[8]罗玉琴,李洋,热依木古丽·阿布都拉,等.RP-HPLC法同时测定石榴皮提取物阴道泡腾片中安石榴苷和鞣花酸[J].中草药,2011,42(10):2011-2013.
[2]毕晓菲,李勇.石榴化学成分及其保健功能的研究进展[J].现代农业科技,2010(22):356-360.
[3]杜欣,叶盛英.石榴叶和石榴皮药理活性研究进展[J].天津药学,2007,19(2):64-66.
[4]林勇.石榴皮的化学、药理与临床研究概述[J].中药材,2010,33(11):1816-1819.
[5]付军,李正.石榴皮鞣质的药理学研究进展[J].中国药业,2011,20(24):92-94.
[6]李建科,李国秀,赵艳红,等.石榴皮多酚组成分析及其抗氧化活性[J].中国农业科学,2009,42(11):4035-4041.
[7]JIMENEZ D R M,RAMAZANOV A,SIKORSKI S,et al.A new method of standartization of health-promoting pomegranate fruit(Punica granatum)extract[J].J Georgian Med News,2006,140:70-77.
[8]罗玉琴,李洋,热依木古丽·阿布都拉,等.RP-HPLC法同时测定石榴皮提取物阴道泡腾片中安石榴苷和鞣花酸[J].中草药,2011,42(10):2011-2013.