类枯草杆菌蛋白酶在植物和病原物互作中的研究进展
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摘要
类枯草杆菌蛋白酶(subtilisin-like proteases,Subtilases)是一类在植物发育和信号级联中实现高度特异性功能的丝氨酸蛋白酶。相关研究表明,几种类枯草杆菌蛋白酶在病原物侵染后被特异性诱导并参与寄主细胞程序性死亡,同时能够在病原物识别和信号转导级联反应中发挥重要功能,但是对其参与植物-病原物互作的具体机理的了解仍然非常有限。本综述对已经成功解析功能的类枯草杆菌蛋白酶相关信息进行整合,概述了其分布、结构、功能等信息,重点突出了其在植物和病原物互作中的作用,旨在为深入理解植物对病原物的防御机制和作物抗病育种提供指导。
Subtilisin-like proteases(subtilases) are a kind of serine proteases that fulfill highly specific functions in plant development and signaling cascades. Related studies have shown that several species of subtilases are specifically induced and contribute to the programmed cell death of plant host after pathogen infection.Simultaneously, they play a pivotal role in pathogen recognition and signal cascade. However, the understanding of its specific mechanisms involved in the interaction of plant pathogens is still very limited. In this article, we integrated the information about the subtilases that had been successfully characterized, and overviewed its information about distribution, structure, function and so on. Importantly, the role of subtilases play in plant and pathogen interaction was emphasized, which would provide guidance for further understanding of plant defense mechanisms and crop disease resistance breeding.
Subtilisin-like proteases(subtilases) are a kind of serine proteases that fulfill highly specific functions in plant development and signaling cascades. Related studies have shown that several species of subtilases are specifically induced and contribute to the programmed cell death of plant host after pathogen infection.Simultaneously, they play a pivotal role in pathogen recognition and signal cascade. However, the understanding of its specific mechanisms involved in the interaction of plant pathogens is still very limited. In this article, we integrated the information about the subtilases that had been successfully characterized, and overviewed its information about distribution, structure, function and so on. Importantly, the role of subtilases play in plant and pathogen interaction was emphasized, which would provide guidance for further understanding of plant defense mechanisms and crop disease resistance breeding.
引文
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Beers E.P.,and Mcdowell J.M.,2001,Regulation and execution of programmed cell death in response to pathogens,stress and developmental cues,Curr.Opin.Plant Biol.,4(6):561-567
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Budic M.,Sabotic J.,Meglic V.,Kos J.,and Kidric M.,2013,Characterization of two novel subtilases from common bean(Phaseolus vulgaris L.)and their responses to drought,Plant Physiol.Biochem.,62:79-87
Camejo D.,Guzmán-Cedeno.,and Moreno A.,2016,Reactive oxygen species,essential molecules,during plant-pathogen interactions,Plant Physiol.Biochem.,103:10-23
Cao J.,Han X.,Zhang T.C.,Yang Y.P.,Huang J.L.,and Hu X.Y.,2014,Genome-wide and molecular evolution analysis of the subtilase gene family in Vitis vinifera,BMC Genomics,15(1):1116
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Dodson G.,and Wlodawer A.,1998,Catalytic triads and their relatives,Trends Biochem.Sci.,23(9):347-352
Dong S.M.,Stam R.,Cano L.M.,Song J.,Sklenar J.,Yoshida K.,Bozkurt T.O.,Oliva R.,Liu Z.Y.,Tian M.Y.,Win J.,Banfield M.J.,Jones A.M.E.,Hoorn R.A.L.,and Kamoun S.,2014,Effector specialization in a lineage of the Irish potato famine pathogen,Science,343(6170):552-555
Duan X.P.,Zhang Z.D.,Wang J.,and Zuo K.J.,2016,Characterization of a novel cotton subtilase gene Gb SBT1 in response to extracellular stimulations and its role in verticillium resistance,PLo S One,11(4):e0153988
Ekchaweng K.,Khunjan U.,and Churngchow N.,2017,Molecular cloning and characterization of three novel subtilisin-like serine protease genes from Hevea brasiliensis,Physiol.Mol.Plant Pathol.,97:79-95
Fakih Z.,Ahmed M.B.,Letanneur C.,and Germain H.,2016,An unbiased nuclear proteomics approach reveals novel nuclear protein components that participates in MAMP-triggered immunity,Plant Signaling and Behavior,11(6):e1183087
Fan T.,Bykova N.V.,Rampitsch C.,and Tim X.,2016,Identification and characterization of a serine protease from wheat leaves,Eur.J.Plant Pathol.,146(2):293-304
Fernández M.B.,Daleo G.R.,and Guevara M.G.,2015,Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity(St SBTc-3),Plant Physiol.Biochem.,86:137-146
Figueiredo A.,Fortes A.M.,Ferreira S.,Sebastiana M.,Choi Y.H.,Sousa L.,Acioli-Santos B.,Pessoa F.,Verpoorte R.,and PaisM.S.,2008,Transcriptional and metabolic profiling of grape(Vitis vinifera L.)leaves unravel possible innate resistance against pathogenic fungi,J.Exp.Bot.,59(12):3371-3381
Figueiredo A.,Monteiro F.,and Sebastiana M.,2014,Subtilisinlike proteases in plant-pathogen recognition and immune priming:a perspective,Front.Plant Sci.,5(739):739
Rantong G.,and Gunawardena A.H.,2015,Programmed cell death:genes involved in signaling,regulation,and execution in plants and animals,Botany-botanique,93(4):193-210
Gindro K.,Berger V.,Godard S.,Voinesco F.,Schnee S.,Viret O.,and Alonso-Villaverde V.,2012,Protease inhibitors decrease the resistance of Vitaceae to Plasmopara viticola,Plant Physiol.Biochem.,60(3):74-80
Hatsugai N.,Iwasaki S.,Tamura K.,Kondo M.,Fuji K.,Ogasawara K.,Nishimura M.,and Hara-Nishimura I.,2009,A novel membrane fusion-mediated plant immunity against bacterial pathogens,Genes Dev.,23(21):2496-2506
Li H.,and Zhang Z.,2016,Systems understanding of plant-pathogen interactions through genome-wide protein-protein interaction networks,Frontiers of Architectural Research,3(2):102-112
Huffaker A.,and Ryan C.A.,2007,Endogenous peptide defense signals in Arabidopsis differentially amplify signaling for the innate immune response,Proc.Natl.Acad.Sci.USA,104(25):10732-10736
Serrano I.,Buscaill P.,Audran C.,Pouzet C.,Jauneau A.,and Rivas S.,2016,A non canonical subtilase attenuates the transcriptional activation of defence responses in Arabidopsis thaliana,Elife,5:e19755
Johansson O.N.,ed,2015,Plant pathogen defense:Signalling,resistance and cell death,Department of Biological and Environmental Sciences,Gteborgs Universitet,Gothenburg,Sweden,pp.6
Jones J.D.G.,and Dangl J.L.,2006,The plant immune system,Nature,444(7117):323-329
JordáL.,Coego A.,Conejero V.,and Vera P.,1999,A genomic cluster containing four differentially regulated subtilisin-like processing protease genes is in tomato plants,J Biol.Chem.,274(4):2360-2365
Kabbage M.,Kessens R.,Bartholomay L.C.,and Williams B.,2017,The life and death of a plant cell,Ann.Rev.Plant Biol.,68(1):375-404
Ksiazek M.,Karim A.Y.,Bryzek D.,Enghild J.J.,Th?gersen I.B.,Koziel J.,and Potempa J.,2015,Mirolase,a novel subtilisinlike serine protease from the periodontopathogen Tannerella forsythia,Biol.Chem.,396(3):261-275
Li D.H.,Xi H.,Yu X.B.,and Cai Y.P.,2015,Molecular cloning and characterization of a subtilisin-like protease from Arabidopsis thaliana,Genet.Mol.Res.,14(4):16535-16545
Liu J.X.,Srivastava R.,and Howell S.,2009,Overexpression of an Arabidopsis gene encoding a subtilase(At SBT5.4)produces a clavata-like phenotype,Planta,230(4):687-697
Meichtry J.,Amrhein N.,and Schaller A.,1999,Characterization of the subtilase gene family in tomato(Lycopersicon esculentum Mill.),Plant Mol.Biol.,39(4):749-760
Meteignier L.V.,Oirdi M.E,Cohen M.,Barff T.,Matteau D.,Lucier J.F.,Rodrigue S.,Jacques P.E.,Yoshioka K.,and Moffett P.,2017,Translatome analysis of an NB-LRRimmune response identifies important contributors to plant immunity in Arabidopsis,J.Exp.Bot.,68(9):2333-2344
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