分子光谱法研究漆黄素与DNA的相互作用
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摘要
利用多种光谱技术及伏安技术研究了漆黄素与DNA之间的相互作用.由荧光光谱法可知,DNA对漆黄素的猝灭过程是静态猝灭,计算得到不同温度下二者的结合常数及结合位点数.ΔH,ΔS,ΔG的计算结果表明漆黄素与DNA的作用力为范德华力或氢键作用.循环伏安法进一步表明了DNA的加入使漆黄素的峰电位发生了移动,二者产生了有效结合.离子强度实验、黏度法、紫外光谱法、圆二色光谱法、DNA熔解温度实验和单双链DNA对比实验的结果均表明漆黄素与DNA的结合是嵌插结合模式.
The interaction between fisetin and DNA was studied using various spectroscopic techniques and voltammetry.Fluorescence spectroscopy showed that the quenching process of fisetin was static quenching,and the binding constant and the number of binding sites at different temperatures were obtained.The calculation results ofΔH,ΔSandΔGshowed that the main binding force was van der Waals force or hydrogen bonding.All the experimental results of ionic strength,viscosity,ultraviolet spectroscopy,circular dichroism spectroscopy,DNA melting and the interaction of fisetin and single stranded DNA point showed that the binding mode between fisetin and DNA was an intercalation binding.
The interaction between fisetin and DNA was studied using various spectroscopic techniques and voltammetry.Fluorescence spectroscopy showed that the quenching process of fisetin was static quenching,and the binding constant and the number of binding sites at different temperatures were obtained.The calculation results ofΔH,ΔSandΔGshowed that the main binding force was van der Waals force or hydrogen bonding.All the experimental results of ionic strength,viscosity,ultraviolet spectroscopy,circular dichroism spectroscopy,DNA melting and the interaction of fisetin and single stranded DNA point showed that the binding mode between fisetin and DNA was an intercalation binding.
引文
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[2]LI M Y,HUANG P C.[J].Chin Pharmacologic Bullet,2012,28(1):88-92.
[3]LIU Y H,ZHOU B,SU H,et al.[J].Chin J Endocrinol Metab,2014,30(2):146-149.
[4]PALEMA M,RICHARD D,JENNIFER L,et al.[J].PLos One,2011,6(6):1-11.
[5]LU H,CHANG D J,BARATTE B,et al.[J].J Med Chem,2005,48:737-743.
[6]GUZZO M R,UEMI M,DONATE P M,et al.[J].J Phys Chem A,2006,110:10545-10551.
[7]KUMAR C V,ASUNCION E H.[J].J Am Chem Soc,1993,115:8547-8553.
[8]LAKOWICZ J R.Principles of fluorescence spectroscopy[M].New York:Plenum Press,1999:237.
[9]WARE W R.[J].J Phys Chem,1962,66:455-458.
[10]VALEUR B,BROVHON J C.New trends in fluorescence spectroscopy[M].Berlin:Springer Press,1999:25.
[11]SUN Y T,ZHANG Q,BI S Y,et al.[J].J Lumin,2011,131:2299-2306.
[12]ROSS P D,SUBRAMANIAN S.[J].Biochem,1981,20:3096-3102.
[13]LONG E C,BARTON J K.[J].Acc Chem Res,1990,23:271-273.
[14]RAJENDRAN A,NAIR B U.[J].BBA-Gen Sub,2006,1760:1794-1801.
[15]KUMAR C V,TURNER R S,ASUNCION E H.[J].J Photochem Photobiol,1993,74(2/3):231-238.
[16]WANG A H J,TENG M K.Molecular recognition of DNA minor groove binding drugs[M].New York:Springer,1990:123.
[17]LERMAN L S.[J].J Mol Biol,1961,3:18-30.
[18]BI S Y,QIAO C Y,QIAN D Q,et al.[J].Sens Actuat B,2006,119:199-208.
[19]张亚锋,吴萍,林新华.[J].化学研究,2009,20(1):37-39.