基于COⅠ及RAG2基因序列的我国科鱼类分子系统研究
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摘要
采集了我国近海分布的、细鳞、尖吻、叉牙、四线列牙等5种科鱼类共25尾样品,利用PCR扩增技术对其线粒体基因COⅠ及核基因RAG2序列片段进行扩增测序,得到5种科鱼类COⅠ基因序列651bp及RAG2基因序列729bp,两基因片段均不存在插入与缺失。利用Mega 5.0计算科鱼类两基因碱基组成情况,种内与种间的遗传距离,并基于最大似然法构建了系统进化树。结果显示,系统进化树上,三线最先分化出来,位于进化树的底部,其次是细鳞,也单独形成一支,独立于其他科鱼类聚类分支之外,同属于属的3个种类并没有形成单系,与格纹岛、尖吻聚为一支;叉牙与四线列牙聚成一支,形成姐妹种,显示两者之间有较近的亲缘关系。
The 651 bp of mitochondrial DNA COⅠsequences and 726 bp of nuclear DNA RAG2 sequences from 25 individuals of 5 Teraponidae fish collected from China Sea were determined in this study.No insertion and deletion existed in the two gene fragments.Base composition and genetic distances among and within species were calculated using Mega 5.0 and the molecular phylogenetic trees were constructed using maximum likelihood methods.The results showed that in the molecular phylogenetic tree,Terapon puta was first separated and rooted at the base of the tree,the next was species T.jarbua,which also formed an independent branch and located outside the other Teraponidae clusters.Three Teraponspecies did not formed an monophyletic group,the last Teraponspecies T.theraps was clustered together with Rhynchopelates oxyrhynchus and Mesopristes cancellatus;Helotes sexlineatus and Pelates quadrilineatus were clustered tightly as sister species,indicating that there were close phylogenetic relationships between the two species.These phylogenetic results might in accordance with the view of Lee and Tsai′s report that T.theraps should be removed from genus Teraponand Helotes was combined with Pelates.
The 651 bp of mitochondrial DNA COⅠsequences and 726 bp of nuclear DNA RAG2 sequences from 25 individuals of 5 Teraponidae fish collected from China Sea were determined in this study.No insertion and deletion existed in the two gene fragments.Base composition and genetic distances among and within species were calculated using Mega 5.0 and the molecular phylogenetic trees were constructed using maximum likelihood methods.The results showed that in the molecular phylogenetic tree,Terapon puta was first separated and rooted at the base of the tree,the next was species T.jarbua,which also formed an independent branch and located outside the other Teraponidae clusters.Three Teraponspecies did not formed an monophyletic group,the last Teraponspecies T.theraps was clustered together with Rhynchopelates oxyrhynchus and Mesopristes cancellatus;Helotes sexlineatus and Pelates quadrilineatus were clustered tightly as sister species,indicating that there were close phylogenetic relationships between the two species.These phylogenetic results might in accordance with the view of Lee and Tsai′s report that T.theraps should be removed from genus Teraponand Helotes was combined with Pelates.
引文
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[25]毛云涛,甘小妮,王绪祯.基于线粒体COⅠ基因的沙鳅亚科鱼类DNA条形码及其分子系统发育研究[J].水生生物学报,2014,38(4):737-744.
[26]刘璐,孙典荣,李纯厚.DNA条形码技术在鲻科鱼类鉴定中的应用[J].中国海洋大学学报:自然科学版,2016,46(11):178-186.
[27]柳淑芳,陈亮亮,戴芳群,等.基于线粒体CO1基因的DNA条形码在石首鱼科(Sciaenidae)鱼类系统分类中的应用[J].海洋与湖沼,2016,41(2):223-232.
[28]Lee S C,Tsai M P.Molecular systematics of the thornfishes genera Teraponand Pelates(Perciformes:Teraponidae)with reference to the new genus Pseudoterapon[J].Zoological Studies,1999,38(3):279-286.
[29]周佳怡,章群,唐优良,等.中国近海科鱼类系统发育初探[J].海洋渔业.2010,23(4):351-355.
[30]Yearsley G K,Last P R,Hoese D F.Standard names of Australian fishes[M].Hobart:CSIRO Marine and Atmospheric Research,2006.
[2]沈世杰.台湾鱼类志[M].台北:台湾大学动物学系出版,1993:360-363.
[3]孟庆闻,苏锦祥,缪学祖.鱼类分类学[M].北京:中国农业出版社,1995,49(2);636-640.
[4]刘瑞玉.中国海洋生物名录[M].北京:科学出版社,2008:886-1066.
[5]Wu H L,Shao K T,Lai C F.Latin-Chinese dictionary of fishes names[M].Taiwan:The Sueichan Press,1999:1028.
[6]Nelson J S.Fishes of the world[M].New York:John Wiley&Sons Inc,2006:360-369.
[7]Fischer W,Whitehead P J P.FAO Species Identification Guide for Fishery Purposes.Eastern Indian Ocean(fishing area 57)and Western Central Pacific(fishing area 71).Volume 4[M].Rome:Food and Agriculture Organization of the United Nations,1974:1-12.
[8]Vari R P.The Terapon perches(Percoidei,Teraponidae),a cladistic analysis and taxonomic revision[J].Bulletin of the American Museum of Natural History,1978,159(5):175-340.
[9]Bohlen J,Slechtova V,Tan H H,et al.Phylogeny of the Southeast Asian freshwater fish genus Pangio(Cypriniformes;Cobitidae)[J].Molecular Phylogenetics and Evolution,2011,61(3):854-865.
[10]Eytan R I,Hastings P A,Holland B R.Reconciling molecules and morphology:molecular systematics and biogeography of Neotropical blennies(Acanthemblemaria)[J].Molecular Phylogenetics and Evolution,2012,62(1):159-173.
[11]Bloom D D,Weir J T,Piller K R,et al.Do freshwater fishes diversify faster than marine fishes A test using state-dependent diversification analyses and molecular phylogenetics of new world silversides(atherinopsidae)[J].Evolution,2013,67(7):2040-2057.
[12]Laakkonen H M,Strelkov P,Lajus D L,et al.Introgressive hybridization between the Atlantic and Pacific herrings(Clupea harengus and C.pallasii)in the north of Europe[J].Marine Biology,2015,162(1):39-54.
[13]Icolas H,Robert H,Louis B.Identification Canadian freshwater fishes through DNA barcodes[J].PloS One,2008,3(6):1-8.
[14]Van G D,Mcblane J F,Ramsden D A,et al.Initiation of V(D)J recombination in a cell-free system[J].Cell,1995,81(6):925-934.
[15]Market E,Papavasiliou F N.V(D)J recombination and the evolution of the adaptive immune system[J].PloS Biology,2003,1(1):16.
[16]Rocha L A,Lindeman K C,Rocha C R,et al.Historical biogeography and speciation in the reef fish genus Haemulon(Teleostei:Haemulidae)[J].Molecular Phylogenetics and Evolution,2008,48(3):918-928.
[17]Cramer C A,Bonatto S L,Reis R E.Molecular phylogeny of the Neoplecostominae and Hypoptopomatinae(Siluriformes:Loricariidae)using multiple genes[J].Molecular Phylogenetics and Evolution.2011,59(1):43-52.
[18]Martinez-Takeshita N,Purcell C M,Chabot C L,et al.A tale of three tails:cryptic speciation in a globally distributed marine fish of the genus Seriola[J].Copeia,2015,103(2):357-368.
[19]Davis A M,Unmack P J,Pusey B J,et al.Marinefreshwater transitions are associated with the evolution of dietary diversification in terapontid grunters(Teleostei:Terapontidae)[J].Journal of Evolutionary Biology,2012,25(6):1163-1179.
[20]Ward R D,Zemlak T S,Innes B H,et al.DNA barcoding Australia′s fish species[J].Philosophical Transactions of the Royal Society B,2005,360(1462):1847-1857.
[21]Thompson J D,Higgins D G,Gibson T J.Clustal W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position specific gap penalties and weight matrix choice[J].Nucleic Acids Research,1994,22(22):4673-4680.
[22]Tamura K,Stecher G,Peterson D,et al.Mega 6:Molecular Evolutionary Genetics Analysis version 6.0[J].Molecular Biology and Evolution,2013,30(12):2725-2729.
[23]Hebert P D N,Cywinska A,Ball S L,et al.Biological identifications through DNA barcodes[J].Proceedings of the Royal Society of London,Series B,2003,270(1512):313-321.
[24]彭居俐,王绪祯,王丁,等.基于线粒体COⅠ基因序列的DNA条形码在鲤科鲌属鱼类物种鉴定中的应用[J].水生生物学报,2009,33(2):271-276.
[25]毛云涛,甘小妮,王绪祯.基于线粒体COⅠ基因的沙鳅亚科鱼类DNA条形码及其分子系统发育研究[J].水生生物学报,2014,38(4):737-744.
[26]刘璐,孙典荣,李纯厚.DNA条形码技术在鲻科鱼类鉴定中的应用[J].中国海洋大学学报:自然科学版,2016,46(11):178-186.
[27]柳淑芳,陈亮亮,戴芳群,等.基于线粒体CO1基因的DNA条形码在石首鱼科(Sciaenidae)鱼类系统分类中的应用[J].海洋与湖沼,2016,41(2):223-232.
[28]Lee S C,Tsai M P.Molecular systematics of the thornfishes genera Teraponand Pelates(Perciformes:Teraponidae)with reference to the new genus Pseudoterapon[J].Zoological Studies,1999,38(3):279-286.
[29]周佳怡,章群,唐优良,等.中国近海科鱼类系统发育初探[J].海洋渔业.2010,23(4):351-355.
[30]Yearsley G K,Last P R,Hoese D F.Standard names of Australian fishes[M].Hobart:CSIRO Marine and Atmospheric Research,2006.