毛冬青叶多酚的提取工艺及其抗氧化性质的研究
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摘要
以毛冬青叶为原料,进行毛冬青叶多酚提取工艺及其抗氧化性活性的研究,建立以没食子酸为标准品,分光光度法测定毛冬青叶多酚含量的分析方法。结果显示:以pH 4的水为溶剂、料液比1∶30(g/mL)、50℃水浴1.5 h条件下提取1次,毛冬青叶多酚得率为2.89%。其多酚化合物对羟基自由基(·OH)和二苯基苦基肼自由基(DPPH·)均有较强的清除效果,最大清除率分别为55.00%和62.85%。
Extraction technology and antioxidant property of polyphenols from leaves of Ilex pubescens was studied using Ilex pubescens as raw materials.The analytical method was established that polyphenols content was determined by UV-spectrophotometric methods with pyrogallic acid as standard. The results showed that the polyphenols extraction rate reaches 2.89 % under the optimized conditions of extraction at 50 ℃ water bath for 1.5 hour once with water(pH value equals 4)as the solvent and the material/solvent ratio of 1 ∶ 30(g/mL). The clearing capacity of polyphenols for hydroxyl free radical and DPPH free radical were very significant by hydroxyl free radical and DPPH free radical scavenging test,were 55.00 % and 62.85 % respectively.
Extraction technology and antioxidant property of polyphenols from leaves of Ilex pubescens was studied using Ilex pubescens as raw materials.The analytical method was established that polyphenols content was determined by UV-spectrophotometric methods with pyrogallic acid as standard. The results showed that the polyphenols extraction rate reaches 2.89 % under the optimized conditions of extraction at 50 ℃ water bath for 1.5 hour once with water(pH value equals 4)as the solvent and the material/solvent ratio of 1 ∶ 30(g/mL). The clearing capacity of polyphenols for hydroxyl free radical and DPPH free radical were very significant by hydroxyl free radical and DPPH free radical scavenging test,were 55.00 % and 62.85 % respectively.
引文
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[3]Peng Wu,Hui Gao,Zhong Huang Li,et al.Two new triterpene saponins from the roots of Ilex pubescens[J].Phytochemistry Letters,2015,12:17-21
[4]Yuan Zhou,Kewu Zeng,Jiayu Zhang,et al.Triterpene saponins from the roots of Ilex pubescens[J].Fitoterapia,2014,97:98-104
[5]Xiao-Qing Chen,Ke Zan,Jie Yang,et al.Quantitative analysis of triterpenoids in different parts of Ilex hainanensis,Ilex stewardii and Ilex pubescens using HPLC-ELSD and HPLC-MSn and antibacterial activity[J].Phytochemistry Letters,2011,126:1454-1459
[6]王明艳,鲁加峰,宋瑛,等.响应面法优化毛冬青根黄酮提取条件[J].食品科学,2011,32(18):81-85
[7]周渊,李宁,张加余,等.RP-HPLC-DAD法同时测定毛冬青根中4种三萜皂苷和2种木脂素的含量[J].中国药学,2014,23(12):866-872
[8]林培燕.毛冬青活性成分及其药代动力学研究[D].北京:北京协和医学院,2013
[9]冯华芬.毛冬青叶化学成分研究[D].桂林:广西师范大学,2012
[10]周渊,周思祥,姜勇,等.毛冬青叶的化学成分研究[J].中草药,2012,43(8):1478-1483
[11]浙江卫生局.浙江民间常用草药[M].杭州:浙江人民出版社,1972
[12]郭新竹,宁正祥.天然酚类化合物及其保健作用[J].食品工业,2002(3):28-29
[13]Jatinder Pal Singh,Amritpal Kaur,Narpinder Singh,et al.In vitro antioxidant and antimicrobial properties of jambolan(Syzygium cumini)fruit polyphenols[J].LWT-Food Science and Technology,2016,65:1025-1030
[14]朱素英.响应面法优化三七花多酚的提取工艺及其抗氧化性分析[J].食品科学,2015,36(10):65-69
[15]唐婷范,刘雄民,凌敏,等.蒜头果皮果肉抗氧化成分提取及其抗氧化性质研究[J].食品科学,2012,33(2):16-19
[16]唐婷范.蒜头果有效成分及其生物活性研究[D].南宁:广西大学,2013:74
[17]孙达旺.植物单宁化学[M].北京:中国林业出版社,1992
[18]欧阳玉祝,陈小东,唐红玉,等.路边青中总多酚的提取与分离研究[J].食品科学,2009,30(16):44-47
[19]赵艳红,李建科,李国秀.天然抗氧化物体外活性评价方法的优选与优化[J].食品科学,2008,29(6):64-69
[20]王广娟.五倍子没食子酸的提取、纯化及抑菌效果研究[D].保定:河北农业大学,2010:38-39