一株烟曲霉抗植物病原菌活性次生代谢产物的研究
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摘要
以一株烟曲霉属白花夹竹桃内生真菌为研究对象,对其抗植物病原菌活性的次生代谢产物进行了研究,共分离鉴定了6个化合物,分别是alternariol(1),4-acetylaminobutyric acid(2),monomethylsulochrin(3),succinic acid(4),cyclo-(Ala-Val)(5),homogentisic acid(6)。化合物1对白菜黑斑病菌、苹果腐烂病菌和葡萄炭疽病菌的抑制率分别为91.8%、89.1%和90.6%,大于阳性对照;对番茄灰霉病菌和黄瓜枯萎病菌的抑制率为86.6%和84.3%,与阳性对照相当;对供试的4种细菌都有很强的抑制作用,整体效果优于阳性对照。本研究为研制新型微生物来源的杀菌剂和植物病害的防治提供了理论依据。
The active metabolites of Aspergillus fumigatus isolated from Neriumindicum Mill. Cv Paihua were studied. All 6 isolated and identified compounds were as follows: alternariol, 4-acetylaminobutyric acid, monomethylsulochrin, succinic acid, cyclo-(ala-val), and homogentisic acid. Alternariol had an inhibitory rate of 91.8%, 89.1%and 90.6% against Alternaria brassicae, Cytospora sp. and Colletotrichum gloeosporioides respectively, higher than that of the positive control. The inhibition rate against Botrytis cirerea and Fusarium oxysporum. sp. cucumebrium Owen was 86.6% and 84.3%, respectively, the same as the positive control. Alternariol had a strong antibacterial effect on all 4 bacteria tested. This study provides a theoretical basis for the development of new microbiological fungicides and plant diseases controlling.
The active metabolites of Aspergillus fumigatus isolated from Neriumindicum Mill. Cv Paihua were studied. All 6 isolated and identified compounds were as follows: alternariol, 4-acetylaminobutyric acid, monomethylsulochrin, succinic acid, cyclo-(ala-val), and homogentisic acid. Alternariol had an inhibitory rate of 91.8%, 89.1%and 90.6% against Alternaria brassicae, Cytospora sp. and Colletotrichum gloeosporioides respectively, higher than that of the positive control. The inhibition rate against Botrytis cirerea and Fusarium oxysporum. sp. cucumebrium Owen was 86.6% and 84.3%, respectively, the same as the positive control. Alternariol had a strong antibacterial effect on all 4 bacteria tested. This study provides a theoretical basis for the development of new microbiological fungicides and plant diseases controlling.
引文
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[2]陈丽媛,孙翠焕.药用植物内生真菌研究进展[J].微生物学杂志,2015,35(5):98-101.
[3]吴征镒.中国植物志[M].北京:科学出版社,1997,63:147.
[4]邢晓娟.夹竹桃的药理作用与临床应用[J].现代医药卫生,2007,23(16):2466.
[5]冯小飞,赵宁,杨品辉,等.红花夹竹桃萃取物对植物病原真菌的抑菌活性[J].福建农业学报,2018,33(8):849-854.
[6]孔阳,李彦军,张雯.蝙蝠葛茎叶石油醚提取物抗菌及食物防腐作用的研究[J].食品科技,2010,35(3):246-248.
[7]Derwich E,Benziane Z,Boukir A.Antibacterial acitivity and chemical composition of the essential oil from flowers of Nerium oleander[J].Ejeafche,Electron J Environ,Agric Food Chem,2010,9(6):1074-1084.
[8]刘少华,陆金萍,朱瑞良,等.一种快速简便的植物病原真菌基因组DNA提取方法[J].植物病理学报,2005,35(4):362-365.
[9]曹永军,程萍,喻国辉,等.利用ITS1和ITS4通用引物扩增香蕉枯萎病菌核酸片段鉴定其生理小种[J].热带作物学报,2010,31(7):1098-1102.
[10]李爱华,岳思君,马海滨.真菌孢子三种计数方法相关性的探讨[J].微生物学杂志,2006,26(2):107-110.
[11]Ma Y M,Qiao K,Kong Y,et al.A new isoquinolone alkaloid from an endophytic fungus R22of Nerium indicum[J].Natural Product Research,2017,31(8):951-958.
[12]刘净,谢涛,等.冬凌草化学成分的研究[J].中国天然药物,2004,5(2):276-279.
[13]Liu J Y,Song Y C,Zhang Z,et al.Aspergillus fumigatus CY018,an endophytic fungus in Cynodon dactylon as a versatile producer of new and bioactive metabolites[J].J biotech,2004,114:279-287.
[14]于德泉,杨峻山.分析化学手册(第二版)[M].北京:化学工业出版社,1999:125-129.
[15]丁中涛,周俊,谭宁华.金铁锁中的四个环二肽[J].中草药,2000,31(11):803-805.
[16]马养民,赵洁.植物内生真菌抗菌活性物质的研究进展[J].有机化学,2010,30(2):220-232.
[17]黄华毅,王佳琳,马荣,等.枯草芽孢杆菌STO-12抑菌活性及其抑菌物质分析[J].浙江农业学报,2017,29(1):81-88.