不同浓度褪黑素刺激对内蒙古绒山羊毛囊干细胞增殖活性的影响
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摘要
试验旨在探究不同浓度褪黑素刺激对内蒙古绒山毛囊干细胞增殖活性的影响。采用0(对照组)、100、300、500、700pg/mL 5个浓度梯度分别对内蒙古绒山羊毛囊干细胞连续刺激5d,MTT法分别检测各组细胞活性。结果表明,不同浓度褪黑素刺激2d后,相较于对照组(0pg/mL),褪黑素刺激组未见成团细胞出现,开始出现长梭形细胞且细胞生长不再均一,各刺激组均表现出较高的增殖活性;刺激4d后,500pg/mL褪黑素刺激组毛囊干细胞增殖活性显著高于0、100pg/mL(P<0.01;P<0.05),但与700pg/mL褪黑素刺激组差异不显著(P>0.05)。因此,500pg/mL是内蒙古绒山羊毛囊干细胞增殖的最适浓度。
This study aimed to investigate the effect of different concentrations of melatonin on proliferative activity of hair follicle stem cells in Inner Mongolia cashmere goat.The five levels melatonin including 0,100,300,500 and 700pg/mL were designed to stimulate the hair follicle stem cells in Inner Mongolia cashmere goat,the MTT method was used to detect cell proliferation activity for 5days.The result showed that 2days later,the groups stimulated by melatonin did not cobble-stone arrangement and began to appear spindle cell and cell growth was not uniform compared with the control group(0pg/mL),the groups showed obvious effect on cell proliferation relatively.4days later,the cell proliferation of groups stimulated by 500pg/mL melatonin were significantly greater than that of 0,100pg/mL(P<0.01;P<0.05),but there was no significant difference with 700pg/mL melatonin stimulation group(P>0.05).The optimal melatonin concentration of hair follicle stem cell proliferation was 500pg/mL in Inner Mongolia cashmere goat.
This study aimed to investigate the effect of different concentrations of melatonin on proliferative activity of hair follicle stem cells in Inner Mongolia cashmere goat.The five levels melatonin including 0,100,300,500 and 700pg/mL were designed to stimulate the hair follicle stem cells in Inner Mongolia cashmere goat,the MTT method was used to detect cell proliferation activity for 5days.The result showed that 2days later,the groups stimulated by melatonin did not cobble-stone arrangement and began to appear spindle cell and cell growth was not uniform compared with the control group(0pg/mL),the groups showed obvious effect on cell proliferation relatively.4days later,the cell proliferation of groups stimulated by 500pg/mL melatonin were significantly greater than that of 0,100pg/mL(P<0.01;P<0.05),but there was no significant difference with 700pg/mL melatonin stimulation group(P>0.05).The optimal melatonin concentration of hair follicle stem cell proliferation was 500pg/mL in Inner Mongolia cashmere goat.
引文
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[13]Moriya T,Horie N,Mitome M,et al.Melatonin influences the proliferative and differentiative activity of neural stem cells[J].J Pineal Res,2007,42(4):411-418.
[14]Gonzalez A,Alvarez-Garca V,Martnez-Campa C.Melatonin promotes differentiation of 3T3-L1 broblasts[J].Pineal Res,2012,52:12-20.
[15]龚凯.褪黑素对大鼠脊髓源性神经干细胞体外培养及诱导分化影响的实验研究[D].西安:中国人民解放军第四军医大学,2007.
[2]Lerner A B,Case J D,Takahashi Y,et al.Isolation of melatonin,the pineal gland factor that lightens melanocytes[J].J Am Chem Soc,1958,80(10):2857-2858.
[3]贾志海.不同光照周期和褪黑激素对绒山羊生产性能的影响[J].中国畜牧杂志,1995,31(4):8-10.
[4]Ibraheem M,Galbraith H,Scaife J,et al.Growth of secondary hair follicles on the Cashmere goat in vitro and their response to protection and melatonin[J].Journal of Anatomy,1994,185(1):135-142.
[5]Wang X,Tredget E,Wu Y.Dynamic signals for follicle development and regeneration[J].Stem Cell and Development,2012,21(1):7-18.
[6]Hardeland R,Tan D X,Reiter R J,et al.Metabolites of melatonin and other indoles:The resurrection of an almost forgotten class of biogenic amines[J].J Pineal Res,2009,47:109-126.
[7]Leston J,Harth C,Brun J,et al.Melatonin is release in the third ventricle in humans.A study in movement disorders[J].Neurosci Lett,2009,469:294-297.
[8]段发亮,陈俊抛.褪黑素对EGF作用后神经干细胞增殖的影响[J].实用医学杂志,2002,18(7):688-690.
[9]Moriya T,Horie N,Mitome M,et al.Melatonin influences the proliferative and differentiative activity of neural stem cells[J].Journal of Pineal Research,2007,42(4):411-418.
[10]Zaminy A,Kashani R,Barbarestani M,et al.Melatonin influences the proliferative and differentiative activity of rat adipose-derived stem cells[J].Cell Journal,2008,10(1):25-32.
[11]Tocharusa C,Puriboriboona Y,Junmaneea T.Melatonin enhances adult rat hippocampal progenitor cell proliferation via ERK signaling pathway through melatonin receptor[J].Neuroscience,2014,275(1):314-321.
[12]陶志华,朱涛.MTT还原法测人淋巴细胞增值活性[J].温州医学院学报,1999,3:49-50.
[13]Moriya T,Horie N,Mitome M,et al.Melatonin influences the proliferative and differentiative activity of neural stem cells[J].J Pineal Res,2007,42(4):411-418.
[14]Gonzalez A,Alvarez-Garca V,Martnez-Campa C.Melatonin promotes differentiation of 3T3-L1 broblasts[J].Pineal Res,2012,52:12-20.
[15]龚凯.褪黑素对大鼠脊髓源性神经干细胞体外培养及诱导分化影响的实验研究[D].西安:中国人民解放军第四军医大学,2007.