毛序准噶尔乌头化学成分的研究
|
摘要
目的研究毛序准噶尔乌头Aconitum soongaricum Stapf var.pubescens Steinb.的化学成分。方法毛序准噶尔乌头80%乙醇提取物的乙酸乙酯部位采用硅胶、反相、Sephadex LH-20、制备柱进行分离纯化,根据波谱数据鉴定所得化合物的结构。结果从中分离得到6个化合物,分别鉴定为山柰酚-7-O-α-L-鼠李糖苷(1)、山柰酚-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖苷(2)、山柰酚-3-O-α-L-阿拉伯糖-7-O-α-L-鼠李糖苷(3)、对羟基肉桂酸甲酯(4)、4-羟基苯基-1-O-β-D-吡喃葡萄糖苷(5)、(3S,5R,6R,7E,9S)-3,5,6,9-tetrahydroxy-7-en-megastigmane(6)。结论所有化合物均为首次从该植物中分离得到。
AIM To study the chemical constituents from Aconitum soongaricum Stapf var. pubescens Steinb..METHODS The ethyl acetate fraction of 80% ethanol extract from Aconitum soongaricum var. pubescens was isolated and purified by silica,ODS,Sephadex LH-20 and preparative column,then the structures of obtained compounds were identified by spectral data. RESULTS Six compounds were isolated and identified as kaempferol-7-O-α-L-rhamnoside(1),kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnoside(2),kaempferol-3-O-α-L-arabinopyranoside-7-O-α-L-rhamnoside(3),p-hydroxy cinnamic methyl ester(4),4-hydroxyphenyl-O-β-D-glucopyranoside(5),(3 S,5 R,6 R,7 E,9 S)-3,5,6,9-tetrahydroxy-7-en-megastigmane(6). CONCLUSION All the compounds are isolated from this plant for the first time.
AIM To study the chemical constituents from Aconitum soongaricum Stapf var. pubescens Steinb..METHODS The ethyl acetate fraction of 80% ethanol extract from Aconitum soongaricum var. pubescens was isolated and purified by silica,ODS,Sephadex LH-20 and preparative column,then the structures of obtained compounds were identified by spectral data. RESULTS Six compounds were isolated and identified as kaempferol-7-O-α-L-rhamnoside(1),kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnoside(2),kaempferol-3-O-α-L-arabinopyranoside-7-O-α-L-rhamnoside(3),p-hydroxy cinnamic methyl ester(4),4-hydroxyphenyl-O-β-D-glucopyranoside(5),(3 S,5 R,6 R,7 E,9 S)-3,5,6,9-tetrahydroxy-7-en-megastigmane(6). CONCLUSION All the compounds are isolated from this plant for the first time.
引文
[1]中国科学院《中国植物志》编辑委员会.中国植物志:第27卷[M].北京:科学出版社,1979:326.
[2]《新疆植物志》编辑委员会.新疆植物志(第2卷):第1册[M].乌鲁木齐:新疆科技卫生出版社,1999:240.
[3]新疆生物土壤沙漠研究所.新疆药用植物志:第1册[M].乌鲁木齐:新疆人民出版社,1977:60-62.
[4]Chen L,Shan L H,Zhang J F,et al.Diterpenoid alkaloids from Aconitum soongaricum var.pubescens[J].Nat Prod Commun,2015,10(12):2063-2065.
[5]李宁,李铣,杨世林,等.过山蕨总黄酮的化学成分研究(1)[J].沈阳药科大学学报,2004,21(2):105-108.
[6]于荣敏,李铣,朱廷如.小花棘豆中黄酮醇和黄酮醇甙的分离鉴定[J].中国中药杂志,1989,14(8):34-36.
[7]Roepke J,Bozzo G G.Arabidopsis thalianaβ-glucosidase BGLU15 attacks flavonol 3-O-β-glucoside-7-O-α-rhamnosides[J].Phytochemistry,2015,109:14-24.
[8]Ozden S,Durust N,Toki K,et al.Acylated kaempferol glycosides from the flowers of Delphinium formosum[J].Phytochemistry,1998,49(1):241-245.
[9]Areej M A,Ghada A F,Shagufta P.Cytotoxic flavonoid glycosides from Rapistrum rugosum L[J].Iran J Pharm Res,2012,11(3):839-844.
[10]邵萌,杨跃辉,高慧媛,等.金荞麦中的酚酸类成分[J].中国中药杂志,2005,30(20):1591-1593.
[11]常忠义,龚维红.丽江蚤缀的化学成分[J].广西植物,2005,25(3):278-280.
[12]Prabhu K,Karar P K,Hemalatha S,et al.Isolation and spectral identification of arbutin from the roots of Viburnum erubescens Wall.ex DC[J].Int J Res Ayurveda Pharm,2011,2(3):889-892.
[13]Takeda Y,Okada Y,Masuda T,et al.New megastigmane and tetraketide from the leaves of Euscaphis japonica[J].Chem Pharm Bull,2000,48(42):752-754.
[14]彭双,韩立峰,刘二伟,等.荷叶中化学成分的分离与鉴定[J].沈阳药科大学学报,2012,29(7):519-524.
[15]郭婕.加拿大一枝黄花的酚类成分和生物活性研究[D].扬州:扬州大学,2008.
[16]外塔尼古丽·卡米力,阿布拉江·克依木.维药蔷薇果总黄酮的提取工艺研究[J].食品研究与开发,2012,33(4):81-84.
[17]Liu C M,Zhao J M,Li H,et al.Supercritical fluid extraction of total flavonoids from leaves of Acanthopanax Senticosus Harms[J].Chem Res Chin Univ,2007,23(2):233-236.
[18]朴英花,朴惠顺.倍半萜类化合物生物活性研究进展[J].职业与健康,2012,28(18):2291-2293.
[2]《新疆植物志》编辑委员会.新疆植物志(第2卷):第1册[M].乌鲁木齐:新疆科技卫生出版社,1999:240.
[3]新疆生物土壤沙漠研究所.新疆药用植物志:第1册[M].乌鲁木齐:新疆人民出版社,1977:60-62.
[4]Chen L,Shan L H,Zhang J F,et al.Diterpenoid alkaloids from Aconitum soongaricum var.pubescens[J].Nat Prod Commun,2015,10(12):2063-2065.
[5]李宁,李铣,杨世林,等.过山蕨总黄酮的化学成分研究(1)[J].沈阳药科大学学报,2004,21(2):105-108.
[6]于荣敏,李铣,朱廷如.小花棘豆中黄酮醇和黄酮醇甙的分离鉴定[J].中国中药杂志,1989,14(8):34-36.
[7]Roepke J,Bozzo G G.Arabidopsis thalianaβ-glucosidase BGLU15 attacks flavonol 3-O-β-glucoside-7-O-α-rhamnosides[J].Phytochemistry,2015,109:14-24.
[8]Ozden S,Durust N,Toki K,et al.Acylated kaempferol glycosides from the flowers of Delphinium formosum[J].Phytochemistry,1998,49(1):241-245.
[9]Areej M A,Ghada A F,Shagufta P.Cytotoxic flavonoid glycosides from Rapistrum rugosum L[J].Iran J Pharm Res,2012,11(3):839-844.
[10]邵萌,杨跃辉,高慧媛,等.金荞麦中的酚酸类成分[J].中国中药杂志,2005,30(20):1591-1593.
[11]常忠义,龚维红.丽江蚤缀的化学成分[J].广西植物,2005,25(3):278-280.
[12]Prabhu K,Karar P K,Hemalatha S,et al.Isolation and spectral identification of arbutin from the roots of Viburnum erubescens Wall.ex DC[J].Int J Res Ayurveda Pharm,2011,2(3):889-892.
[13]Takeda Y,Okada Y,Masuda T,et al.New megastigmane and tetraketide from the leaves of Euscaphis japonica[J].Chem Pharm Bull,2000,48(42):752-754.
[14]彭双,韩立峰,刘二伟,等.荷叶中化学成分的分离与鉴定[J].沈阳药科大学学报,2012,29(7):519-524.
[15]郭婕.加拿大一枝黄花的酚类成分和生物活性研究[D].扬州:扬州大学,2008.
[16]外塔尼古丽·卡米力,阿布拉江·克依木.维药蔷薇果总黄酮的提取工艺研究[J].食品研究与开发,2012,33(4):81-84.
[17]Liu C M,Zhao J M,Li H,et al.Supercritical fluid extraction of total flavonoids from leaves of Acanthopanax Senticosus Harms[J].Chem Res Chin Univ,2007,23(2):233-236.
[18]朴英花,朴惠顺.倍半萜类化合物生物活性研究进展[J].职业与健康,2012,28(18):2291-2293.