乙型肝炎病毒感染者血清HBV-LP检测意义及其与PreS1抗原、HBV DNA之间关系的研究
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摘要
目的初步探讨HBV感染者血清中的乙肝大蛋白(HBV-LP)检测的临床意义,并对其与Pre S1抗原、HBV DNA之间的关系进行分析。方法采用酶联免疫吸附试验(ELISA)方法检测192例HBV感染者血清中的HBV-LP,Pre S1抗原及HBV分子标志物(HBV M),并用荧光定量PCR方法检测HBV DNA。结果 192例HBV感染者血清HBVLP、Pre S1抗原、HBV DNA及HBe Ag阳性率分别为67.18%、54.17%、52.60%和9.38%,HBV-LP阳性率高于Pre S1抗原阳性率(χ~2=6.82,P<0.05)、HBV DNA检测阳性率(χ~2=8.50,P<0.05)及HBe Ag阳性率(χ~2=135.80,P<0.05);HBVLP含量(A值)与HBV DNA拷贝数的对数值呈正相关关系(r=0.798,P<0.05)。结论血清中HBV-LP的含量与HBV DNA的拷贝数相关性较好,HBV-LP能准确的反映乙肝病毒复制情况,是HBe Ag、Pre S1抗原的重要补充。
Objective To preliminarily explore the clinical value of serum hepatitis B virus large surface protein( HBV- LP)detection,and to analyze the relationship between HBV- LP and Pre S1 antigen,HBV DNA copies. Methods Enzyme linked immuno- sorbent assay( ELISA) was used to detect HBV- LP,Pre S1- Ag and molecular markers of HBV in 192 serum samples of HBV patients,and HBV DNA was quantitatively determined by real- time polymerase chain reaction( PCR). Results Among 192 serum samples of HPV patients,the positive rates of HBV- LP,Pre S1- Ag,HBV DNA,and HBe Ag were 67. 18%,54. 17%,52. 60% and 9. 38% respectively. The positive rate of HBV- LP was higher than those of Pre S1- Ag( χ~2= 6. 82,P <0. 05),HBV DNA( χ~2= 8. 50,P < 0. 05),and HBe Ag( χ~2= 135. 80,P < 0. 05). The content of HBV- LP( A value) was positively correlated with the logarithm value of the number of HBV DNA copies( r = 0. 798,P < 0. 05). Conclusions There was a good correlation between the content of serum HBV- LP and the number of HBV DNA copies. Serum HBV- LP can accurately reflect the state of HBV DNA reproduction,and it is an important complementarity marker to traditional HBe Ag and Pre S1- Ag.
Objective To preliminarily explore the clinical value of serum hepatitis B virus large surface protein( HBV- LP)detection,and to analyze the relationship between HBV- LP and Pre S1 antigen,HBV DNA copies. Methods Enzyme linked immuno- sorbent assay( ELISA) was used to detect HBV- LP,Pre S1- Ag and molecular markers of HBV in 192 serum samples of HBV patients,and HBV DNA was quantitatively determined by real- time polymerase chain reaction( PCR). Results Among 192 serum samples of HPV patients,the positive rates of HBV- LP,Pre S1- Ag,HBV DNA,and HBe Ag were 67. 18%,54. 17%,52. 60% and 9. 38% respectively. The positive rate of HBV- LP was higher than those of Pre S1- Ag( χ~2= 6. 82,P <0. 05),HBV DNA( χ~2= 8. 50,P < 0. 05),and HBe Ag( χ~2= 135. 80,P < 0. 05). The content of HBV- LP( A value) was positively correlated with the logarithm value of the number of HBV DNA copies( r = 0. 798,P < 0. 05). Conclusions There was a good correlation between the content of serum HBV- LP and the number of HBV DNA copies. Serum HBV- LP can accurately reflect the state of HBV DNA reproduction,and it is an important complementarity marker to traditional HBe Ag and Pre S1- Ag.
引文
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[13]熊俊,刘欧,石文静.e抗原阴性慢性乙肝患者血清中乙肝表面抗原大蛋白的表达[J].现代检验医学杂志,2007,22(3):35-36.
[14]吴正林,陆学东,钟小强,等.HBe Ag阳性患者乙型肝炎表面大蛋白水平的变化及其与ALT的关系[J].中国实验诊断学,2008,12(3):890-892.
[15]王志东,王亚,李颢.乙型肝炎大蛋白(LHBs)在慢性乙型肝炎患者中检测结果分析[J].国际检验医学杂志,2011,32(14):1628-1629.
[16]Yamada T,Iwabuki H,Kanno T,et al.Physicolchemical and immunological characterization of HBV envelope particles[J].Vaccine,2001,19(4):3154-3163.
[17]徐爱芳,陈刚,张永乐,等.乙型肝炎病毒表面大蛋白研究进展[J].肝脏杂志,2009,14(6):505-506.
[2]蔡兰兰,祝玲玲.乙肝HBV DNA荧光定量检测与乙肝五项及Pres1相关性分析[J].实用预防医学,2014,21(10):1171-1172.
[3]卜国平,开远胜.乙型肝炎病毒潜S1抗原的实验室检测及其应用[J].实用肝病杂志,2007,10(4):250-251.
[4]Barrera A,Guerra B,Notvall L,et al.Mapping of the hepatitis B virus pre-S1 domain involved in receptor recognition[J].J Virol,2005,79(15):9786-9798.
[5]中华医学会肝病学分会,中华医学会感染病学分会.慢性乙肝防治指南[J].医药导报,2006,25(2):116-120.
[6]毛远丽,李伯安,马洪滨,等.乙肝患者外膜蛋白血清学检测及对于判定HBV DNA复制的意义[J].中华实验和临床病毒学杂志,2006,20(3):276-278.
[7]梁晓峰,陈园生,王晓军,等.中国3岁以上人群乙型肝炎血清流行病学研究[J].中华流行病学杂志,2005,26(3):655-658.
[8]Lambert C,Mann S,Prange R.Assessment of determinants affecting the dual topology of hepadnaviral large envelope proteins[J].J Gen Virol,2004,85(pt5):1221-1225.
[9]贾继东.乙型肝炎e抗原阴性慢性乙型肝炎的治疗[J].中华肝脏病杂志,2005,5(13):539.
[10]Stoeckl L,Funk A,Kopitzki A,et al.Identification of a structural motif crucial for infectivity of hepatitis B viruses[J].Proc Natl Acad Sci USA,2006,103:6730-6734.
[11]刘键,蒋英,吴正林,等.乙型肝炎患者血清乙型肝炎病毒外膜大蛋白(LHBs)的检测分析[J].实用预防医学,2014,21(5):607-609.
[12]Paran N,Cooper A,Shaul Y.Interaction of HBV with cells[J].Rev Med Viol,2003,13(3):137-143.
[13]熊俊,刘欧,石文静.e抗原阴性慢性乙肝患者血清中乙肝表面抗原大蛋白的表达[J].现代检验医学杂志,2007,22(3):35-36.
[14]吴正林,陆学东,钟小强,等.HBe Ag阳性患者乙型肝炎表面大蛋白水平的变化及其与ALT的关系[J].中国实验诊断学,2008,12(3):890-892.
[15]王志东,王亚,李颢.乙型肝炎大蛋白(LHBs)在慢性乙型肝炎患者中检测结果分析[J].国际检验医学杂志,2011,32(14):1628-1629.
[16]Yamada T,Iwabuki H,Kanno T,et al.Physicolchemical and immunological characterization of HBV envelope particles[J].Vaccine,2001,19(4):3154-3163.
[17]徐爱芳,陈刚,张永乐,等.乙型肝炎病毒表面大蛋白研究进展[J].肝脏杂志,2009,14(6):505-506.