高脂日粮对小鼠不同部位脂肪沉积及血液生化指标的影响
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摘要
为了研究高脂日粮对小鼠脂肪沉积及血液生化指标的影响,选取3周龄C57BL/6J小鼠12只,随机分为高脂日粮组和低脂日粮组(对照组),每组6只。饲喂11 w后,测体重、采食量并采集血样和不同部位脂肪组织及肝脏。采用冰冻切片HE染色方法检测脂肪细胞的充脂情况。结果显示,高脂日粮组与对照组相比,体重极显著增加(P<0.01),腹股沟脂肪和附睾脂肪重量显著增加(P<0.05),脂肪细胞明显增大,脂解基因和产热基因表达显著降低(P<0.05),血液中T-chol和HDL-c含量显著升高(P<0.05),ALT与AST水平显著降低(P<0.05),但AST/ALT比值偏高。以上结果表明,高脂日粮引起的肥胖是由于脂肪细胞肥大、产热基因和脂解基因表达降低以及不同部位脂肪沉积增多造成的,并且易引起肝脏炎症。
This study was designed to investigate the effect of high-fat diets on lipid deposition and blood biochemical indexes in mice. Twelve C57 BL/6 J mice aged 3 weeks were randomly divided into two groups: high-fat diet group and low-fat diet group(control group), each group of 6. After 11 weeks of feeding, body weight and feed intake were measured, and blood samples and different parts of adipose tissue and liver were collected. Frozen section HE staining was used to detect adipose cells. The results showed that compared with the control group, the body weight of the high-fat diet group significantly increased(P<0.01), so did the weight of inguinal fat and epididymal fat as well as the adipocytes(P<0.05); the expression of lipolysis genes and thermogenesis genes decreased significantly(P<0.05); the T-chol HDL-c levels were significantly increased and ALT and AST levels significantly lowered in blood(P<0.05), but the AST/ALT ratio was higher. The above results indicate that the causes of obesity in high-fat diets are adipose cells hypertrophy, decreased expression of thermogenic genes and lipolysis genes, and increased fat deposition in different parts of the body, which is prone to liver inflammation.
This study was designed to investigate the effect of high-fat diets on lipid deposition and blood biochemical indexes in mice. Twelve C57 BL/6 J mice aged 3 weeks were randomly divided into two groups: high-fat diet group and low-fat diet group(control group), each group of 6. After 11 weeks of feeding, body weight and feed intake were measured, and blood samples and different parts of adipose tissue and liver were collected. Frozen section HE staining was used to detect adipose cells. The results showed that compared with the control group, the body weight of the high-fat diet group significantly increased(P<0.01), so did the weight of inguinal fat and epididymal fat as well as the adipocytes(P<0.05); the expression of lipolysis genes and thermogenesis genes decreased significantly(P<0.05); the T-chol HDL-c levels were significantly increased and ALT and AST levels significantly lowered in blood(P<0.05), but the AST/ALT ratio was higher. The above results indicate that the causes of obesity in high-fat diets are adipose cells hypertrophy, decreased expression of thermogenic genes and lipolysis genes, and increased fat deposition in different parts of the body, which is prone to liver inflammation.
引文
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[2] BELACHEW T.Are we ready for the rising silent epidemic of metabolic syndrome and chronic non-communicable disease inethiopia [J].Ethiop J Health Sci,2015,25(1):1-2.
[3] JEFFERY RW,HARNACK L J.Evidence implicating eating as a primary driver for the obesity epidemic[J].Diabetes,2007,56(11):2 673-2 676.
[4] SAMUELS L T,GILMORE L G,REINECKE R M.The effect of previous diet on the ability of animals to do work during subsequent fasting[J].J Nutr,1948,36(5):639-647.
[5] SAMUELS L T,REINECKE R M,BALL H A.Effect of diet on glucose tolerance and liver and muscle glycogen of hypophysectomized and normal rats[J].Endocrinology,1942,31:42-53.
[6] BUETTNER R,SCH?LMERICH J,BOLLHEIMER L C.High-fat diets:modeling the metabolic disorders of human obesity in rodents[J].Obesity,2007,15(4):798-808.
[7] PECKHAM S C,ENTENMANN C,CARROLL H W.The influence of a hypercaloric diet on gross body and adipose tissue composition in the rat[J].J Nutr,1962,77(2):187-197.
[8] BELZUNG F,RACLOT T,GROSCOLAS R.Fish oil n-3 fatty acids selectively limit the hypertrophy of abdominal fat depots in growing rats fed high-fat diets [J].Am J Physiol,1993,264(6):1 111-1 118.
[9] CORBETT S W,STERN J S,KEESEY R E.Energy expenditure in rats with diet-induced obesity [J].Am J Clin Nutr,1986,44(2):173-180.
[10] TEPPERMAN H M,DEWITT J,TEPPERMAN,J.Effect of a high fat diet on rat adipocyte lipolysis:responses to epinephrine,forskolin,methylisobutylxanthine,dibutyryl cyclic AMP,insulin and nicotinic acid[J].J Nutr,1986,116(10):1 984-1 991.
[11] MORAES R C,BLONDET A,BIRKENKAMP-DEMTROEDER K,et al.Study of the alteration of gene expression in adipose tissue of diet-induced obese mice by microarray and reverse transcription-polymerase chain reaction analyses[J].Endocrinology,2003,144(11):4 773-4 782.
[12] TAKAHASHI Y,IDE T.Effect of dietary fats differing in degree of unsaturation on gene expression in rat adipose tissue[J].Ann Nutr Metab,1999,43(2):86-97.
[13] ROSEN E D,SPIEGELMAN B M.What we talk about when we talk about fat[J].Cell,2014,156(1):20-44.
[14] SURWIT R S,KUHN C M,COCHRANE C,et al.Diet-induced type IIdiabetes in C57BL/6J mice[J].Diabetes,1988,37(9):1 163-1 167.
[15] HARTE R A,KIRK E A,ROSENFELD M E,et al.Initiation of hyperinsulinemia and hyperleptinemia is diet dependent in C57BL/6 mice[J].Horm Metab Res,1999,31(10):570-575.
[16] TAKAHASHI Y,SOEJIMA Y,FUKUSATO T.Animal models of nonalcoholic fatty liver disease nonalcoholic steatohepatitis[J].World J Gastroenterol,2012,18(19):2 300-2 308.
[17] DENG Q G,SHE H,CHENG J H,et al.Steatohepatitis induced by intragastric overfeeding in mice[J].Hepatology,2005,42(4):905-914.
[18] LIEBER C S,LEO M A,MAK K M,et al.Model of nonalcoholic steatohepatitis[J].Am J Clin Nutr,2004,79(3):502-509.
[19] ITO M,SUZUKI J,TSUJIOKA S,et al.Longitudinal analysis of murine steatohepatitis model induced by chronic exposure to high-fat diet[J].Hepatol Res,2007,37(1):50-57.
[20] HARROLD J A,WIDDOWSON P S,Clapham J C,et al.Individual severity of dietary obesity in unselected Wistar rats:relationship with hyperphagia[J].Am J Physiol Endocrinol Metab,2000,279(2):E340-347.
[21] MAEGAWA H,KOBAYASHI M,ISHIBASHI O,et al.Effect of diet change on insulin action:difference between muscles and adipocytes[J].Am J Physiol,1986,251(5 Pt 1):E616-623.
[22] OAKES N D,COONEY G J,CAMILLERI S,et al.Mechanisms of liver and muscle insulin resistance induced by chronic high-fat feeding[J].Diabetes,1997,46(11):1 768-1 774.
[23] ROMESTAING C,PIQUET M A,BEDU E,et al.Long term highly saturated fat diet does not induce NASH in Wistar rats[J].Nutr Metab(lond),2007(4):4-17.