不同产地白及遗传多态性的RAPD分析
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摘要
目的研究国内不同产地白及的遗传多样性和亲缘关系。方法利用随机扩增多态DNA(randomamplified polymorphic DNA,RAPD)引物,RAPD分子标记技术分析国内50个不同产地的白及。结果 RAPD技术扩增产物经琼脂凝胶电泳检测,从50条引物中筛选出10条(S8,S9,S14,S19,S23,S25,S28,S29,S30,S31)条带清晰的引物,10条引物共扩增出88条DNA条带,其中多态性的DNA条带数目为81条,占总数的92.05%。每个引物能扩增出5～11条DNA条带,平均可扩增出8.8条;扩增最少的引物为S19,扩增出5条DNA条带;扩增最多的引物为S29,扩增出11条DNA条带。而每个引物能扩增的多态性DNA条带数为3～11条,平均可扩增出8.6条。结论不同产地的白及具有较丰富的遗传多样性,RAPD可有效应用于白及的遗传多样性研究。
OBJECTIVE To explore the genetic diversities and relationship of Bletilla striata from different habitats.METHODS Random amplified polymorphic DNA(RAPD) primers were used to analyze the Bletilla striata from 50 different areas with RAPD molecular marker technique. RESULTS The clear bands of S8, S9, S14, S19, S23, S25, S28, S29, S30 and S31 amplified by RAPD molecular marker technique and analyzed by agarose gel electrophoresis were screened from 50 primers.The 88 DNA fragments were amplified by these 10 primers and 92.05% of these DNA fragments were polymorphic. Each primer was able to amplify 5 to 11 DNA fragments with an average of 8.8 amplified. The amplification number of S19 primers was the least, only 5 fragments and the amplification number of S29 primers was the most with 11 fragments. Each primer was able to amplify 3 to 11 polymorphic DNA fragments with an average of 8.6 amplified. CONCLUSION There is obvious polymorphism and genetic difference among the Bletilla striata from different areas. RAPD markers exhibite efficiencies for fingerprinting Bletilla sfriata genotypes.
OBJECTIVE To explore the genetic diversities and relationship of Bletilla striata from different habitats.METHODS Random amplified polymorphic DNA(RAPD) primers were used to analyze the Bletilla striata from 50 different areas with RAPD molecular marker technique. RESULTS The clear bands of S8, S9, S14, S19, S23, S25, S28, S29, S30 and S31 amplified by RAPD molecular marker technique and analyzed by agarose gel electrophoresis were screened from 50 primers.The 88 DNA fragments were amplified by these 10 primers and 92.05% of these DNA fragments were polymorphic. Each primer was able to amplify 5 to 11 DNA fragments with an average of 8.8 amplified. The amplification number of S19 primers was the least, only 5 fragments and the amplification number of S29 primers was the most with 11 fragments. Each primer was able to amplify 3 to 11 polymorphic DNA fragments with an average of 8.6 amplified. CONCLUSION There is obvious polymorphism and genetic difference among the Bletilla striata from different areas. RAPD markers exhibite efficiencies for fingerprinting Bletilla sfriata genotypes.
引文
[1]中国药典.一部[S].2015:103.
[2]WANG C Q,XIA T,CHEN H,et al.Metabolism of militarine component of the effective fractions of Bletilla striata in zebrafish[J].Chin J New Drugs(中国新药杂志),2019,28(4):467-472.
[3]WAN D Q,ZHAO R Q,LIU H,et al.The constituents,pharmacological action and clinical application of Bletilla striata[J].China Pharm(中国药业),2017,26(2):93-96.
[4]KALKI?IM O,OKCU M,OKCU Z,et al.Relationships among some pears genotypes(Pyrus communis L.)based on ISSR and RAPD analysis[J].Erwerbs-Obstbau,2016,58(4):259-264.
[5]WANG P F,LI L F,DU J J,et al.Genetic diversity among wild Chinese dwarf cherry from different regions and its closely related plant species based on RAPD analysis[J].JPlant Genet Resour(植物遗传资源学报),2015,16(1):119-126.
[6]XU P,LIANG W Q,ZHOU J,et al.Research on DNAmolecular markers in germplasm resources of Paeonia lactiflora Pall.[J].Chin Arch Tradit Chin Med(中华中医药学刊),2017,35(4):949-953.
[7]王健胜,侯桂玲,谢永凤.国内外苜蓿品种遗传多样性RAPD分析[J].江苏农业科学,2017,45(13):35-38.
[8]周涛,江维克,魏升华,等.野生白芨的资源调查和利用现状分析[C]//中华中医药学会第九届中药鉴定学术会议论文集.浙江建德,2008:270-279.
[9]LU X X.Design and construction of plant germplasm Banks[J].Chin Bull Bot(植物学通报),2006,23(1):119-125.
[10]WILLIAMS J G,KUBELIK A R,LIVAK K J,et al.DNApolymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Res,1990,18(22):6531-6535.
[11]TAN Y,LI Z H,LI J L,et al.Application of RAPD molecular marker technology in plant research[J].J Anhui Agric Sci(安徽农业科学),2013,41(25):10236-10238.
[12]MA Y Z,CHEN G P.Analysis of genetic diversity in eleven varieties of Schizonepeta tenuifolia by RAPD[J].J Chin Med Mater(中药材),2017,40(2):311-314.
[13]LI M,HUANG L M,CHEN Q.The screening of Ohpiopogon Japonicus molecular marker by RAPD analysis[J].J Zhejiang Univ Technol(浙江工业大学学报),2014,42(5):504-508.
[14]HU T M,HAN B,HU X N,et al.Analysis of genetic relationship of 42 Alfalfa varieties by RAPD[J].J Northeast Agric Sci(东北农业科学),2017,42(5):30-35.
[2]WANG C Q,XIA T,CHEN H,et al.Metabolism of militarine component of the effective fractions of Bletilla striata in zebrafish[J].Chin J New Drugs(中国新药杂志),2019,28(4):467-472.
[3]WAN D Q,ZHAO R Q,LIU H,et al.The constituents,pharmacological action and clinical application of Bletilla striata[J].China Pharm(中国药业),2017,26(2):93-96.
[4]KALKI?IM O,OKCU M,OKCU Z,et al.Relationships among some pears genotypes(Pyrus communis L.)based on ISSR and RAPD analysis[J].Erwerbs-Obstbau,2016,58(4):259-264.
[5]WANG P F,LI L F,DU J J,et al.Genetic diversity among wild Chinese dwarf cherry from different regions and its closely related plant species based on RAPD analysis[J].JPlant Genet Resour(植物遗传资源学报),2015,16(1):119-126.
[6]XU P,LIANG W Q,ZHOU J,et al.Research on DNAmolecular markers in germplasm resources of Paeonia lactiflora Pall.[J].Chin Arch Tradit Chin Med(中华中医药学刊),2017,35(4):949-953.
[7]王健胜,侯桂玲,谢永凤.国内外苜蓿品种遗传多样性RAPD分析[J].江苏农业科学,2017,45(13):35-38.
[8]周涛,江维克,魏升华,等.野生白芨的资源调查和利用现状分析[C]//中华中医药学会第九届中药鉴定学术会议论文集.浙江建德,2008:270-279.
[9]LU X X.Design and construction of plant germplasm Banks[J].Chin Bull Bot(植物学通报),2006,23(1):119-125.
[10]WILLIAMS J G,KUBELIK A R,LIVAK K J,et al.DNApolymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Res,1990,18(22):6531-6535.
[11]TAN Y,LI Z H,LI J L,et al.Application of RAPD molecular marker technology in plant research[J].J Anhui Agric Sci(安徽农业科学),2013,41(25):10236-10238.
[12]MA Y Z,CHEN G P.Analysis of genetic diversity in eleven varieties of Schizonepeta tenuifolia by RAPD[J].J Chin Med Mater(中药材),2017,40(2):311-314.
[13]LI M,HUANG L M,CHEN Q.The screening of Ohpiopogon Japonicus molecular marker by RAPD analysis[J].J Zhejiang Univ Technol(浙江工业大学学报),2014,42(5):504-508.
[14]HU T M,HAN B,HU X N,et al.Analysis of genetic relationship of 42 Alfalfa varieties by RAPD[J].J Northeast Agric Sci(东北农业科学),2017,42(5):30-35.