基于铜掺杂碳纳米点荧光测定炭疽生物标志物
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摘要
以柠檬酸、乙二胺为前体,硫酸铜为金属掺杂剂,采用一步水热法制备了一种高荧光量子产率的水溶性铜掺杂碳纳米点(Cu-CDs)。根据2,6-吡啶二甲酸(DPA)与碳纳米点的强螯合作用建立了铜掺杂碳纳米点荧光猝灭测定炭疽生物标志物DPA的新方法。在最佳实验条件下,DPA在5～100 nmol/L(r~2=0.994 1)和150～400 nmol/L(r~2=0.997 6)浓度范围内与Cu-CDs的荧光猝灭率呈良好的线性关系,检出限为2.3 nmol/L。该分析方法成本低、专属性强、灵敏度高、操作简便,在炭疽生物标志物检测方面具有良好的应用前景。
In this study,water-soluble copper-doped carbon nanodots(Cu-CDs) with high fluorescence quantum yield were prepared by one-step hydrothermal method using citric acid and ethylenediamine as precursors and copper sulfate as metal dopants.A new method for the fluorescence determination of anthrax biomarker based on copper-doped carbon nanodots was established based on the strong chelation of 2,6-pyridinedicarboxylic acid(DPA) with carbon nanodots.Under the optimum experimental conditions,there were good linear relationships for the fluorescence quenching rate of Cu-CDs with DPA in the concentration ranges of 5-100 nmol/L(r~2=0.994 1) and 150-400 nmol/L(r~2=0.997 6),and the detection limit of the proposed method was 2.3 nmol/L.With the advantages of low cost,high specificity,high sensitivity and simple operation,the method has a good application prospect in detection of anthrax biomarker.
In this study,water-soluble copper-doped carbon nanodots(Cu-CDs) with high fluorescence quantum yield were prepared by one-step hydrothermal method using citric acid and ethylenediamine as precursors and copper sulfate as metal dopants.A new method for the fluorescence determination of anthrax biomarker based on copper-doped carbon nanodots was established based on the strong chelation of 2,6-pyridinedicarboxylic acid(DPA) with carbon nanodots.Under the optimum experimental conditions,there were good linear relationships for the fluorescence quenching rate of Cu-CDs with DPA in the concentration ranges of 5-100 nmol/L(r~2=0.994 1) and 150-400 nmol/L(r~2=0.997 6),and the detection limit of the proposed method was 2.3 nmol/L.With the advantages of low cost,high specificity,high sensitivity and simple operation,the method has a good application prospect in detection of anthrax biomarker.
引文
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