特应性皮炎患者外周血中Th22、Treg细胞及其细胞因子表达的研究
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摘要
目的检测特应性皮炎(AD)患者外周血中辅助性T细胞22(Th22)和调节性T细胞(Treg)及其细胞因子IL-22和TGF-β的表达,探讨其与AD发病之间的关系及临床意义。方法选择52例AD患者,按照欧洲特应性皮炎评分标准(SCORAD)进行评分分组;使用流式细胞仪检测52例AD患者和和20例健康人Th22和Treg细胞的数量;用酶联免疫吸附法(ELISA)检测血清中IL-22和TGF-β的表达。结果与对照组比较,AD患者外周血中Th22细胞的比例及血清中IL-22的水平显著增高(P<0.0001),Treg细胞的比例及血清中TGF-β的水平显著降低(P<0.0001);与中度AD患者比较,重度AD患者外周血Th22细胞的比例及血清中IL-22的水平显著增高(P<0.0001),Treg细胞的比例及血清中TGF-β的水平显著降低(P<0.0001)。结论 AD患者外周血中Th22细胞及其相关细胞因子表达水平显著升高,而Treg细胞及其相关细胞因子的表达降低,提示Th22和Treg可能在AD的发病机制中发挥重要作用。
Objective To detect the variations of T helper 22 cells(Th22)and regulatory T cells(Treg)and its related cytokines interleukin(IL)-22 and transforming growth factor(TGF-β)in peripheral blood of the patients with atopic dermatitis(AD).Methods 52 patients with AD were recruited and grouped according to the European atopic dermatitis criteria rating(SCORAD).The percentages of Th22 and Treg cells in 52 AD patients and 20 control group was detected by flow cytometric.The concentrations of IL-22 and TGF-βin serum was detected by enzyme-linked immunosorbent assay(ELISA).Results The proportion of Th22 cells in peripheral blood and the level of IL-22 in serum of patients with AD weresignificantly higher than normal people(P<0.05,P<0.0001).The proportion of Th22 cells and the level of IL-22 in severe AD patients were higher than those in moderate AD patients(P<0.05,P<0.0001).In addition,the proportion of Treg cells in peripheral blood and the level of TGF-βserum of patients with AD were significantly lower than those in normal patients(P<0.05,P<0.0001).The proportion of Treg cells and the level of TGF-βin patients with severe AD were significantly lower than those in moderate AD patients(P<0.05,P<0.0001).Conclusion The study suggests that Th22 and Treg cell might play an important role in the pathogenesis of AD.
Objective To detect the variations of T helper 22 cells(Th22)and regulatory T cells(Treg)and its related cytokines interleukin(IL)-22 and transforming growth factor(TGF-β)in peripheral blood of the patients with atopic dermatitis(AD).Methods 52 patients with AD were recruited and grouped according to the European atopic dermatitis criteria rating(SCORAD).The percentages of Th22 and Treg cells in 52 AD patients and 20 control group was detected by flow cytometric.The concentrations of IL-22 and TGF-βin serum was detected by enzyme-linked immunosorbent assay(ELISA).Results The proportion of Th22 cells in peripheral blood and the level of IL-22 in serum of patients with AD weresignificantly higher than normal people(P<0.05,P<0.0001).The proportion of Th22 cells and the level of IL-22 in severe AD patients were higher than those in moderate AD patients(P<0.05,P<0.0001).In addition,the proportion of Treg cells in peripheral blood and the level of TGF-βserum of patients with AD were significantly lower than those in normal patients(P<0.05,P<0.0001).The proportion of Treg cells and the level of TGF-βin patients with severe AD were significantly lower than those in moderate AD patients(P<0.05,P<0.0001).Conclusion The study suggests that Th22 and Treg cell might play an important role in the pathogenesis of AD.
引文
[1]赵漂萍,余红.特应性皮炎的治疗进展[J].实用医学杂志,2015,31(13):2104.
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[12]Kuo I,Carpentermendini A,Yoshida T,et al.Activation of epidermal toll-like receptor 2enhances tight junction function-Implications for atopic dermatitis and skin barrier repair[J].Journal of Investigative Dermatology,2013,133(4):988.
[13]Fujita H,Nograles K E,Kikuchi T,et al.Human Langerhans cells induce distinct IL-22-producing CD4+T cells lacking IL-17production[J].Proc Natl Acad Sci U S A,2009,106(51):21795.
[14]Mu Z,Zhao Y,Liu X,et al.Molecular Biology of Atopic Dermatitis[J].Clinical Reviews in Allergy&Immunology,2014,47(2):193.
[15]Cavani A,Pennino D,Eyerich K.Th17and Th22in Skin Allergy[J].Chemical Immunology&Allergy,2012,96(96):39.
[16]Auriemma M,Vianale G,Amerio P,et al.Cytokines and T cells in atopic dermatitis[J].European Cytokine Network,2013,24(1):37.
[17]Fujita H.Role of IL-22in the pathogenesis of skin diseases[J].Nihon Rinsho Meneki Gakkai Kaishi,2012,35(3):168.
[18]任小丽,苏湘川,石青青,等.Th22细胞及IL-22在特应性皮炎患者外周血中的表达及意义[J].中国中西医结合皮肤性病学杂志,2017,16(2):100.
[19]SangChul Han,DongHwan Koo,NaJin Kang,et al.Docosahexaenoic Acid Alleviates Atopic Dermatitis by Generating Treg and IL-10|[sol]|TGF-|[beta]|-Modified Macrophage via TGF-|[beta]|[J].Dependent Mechanism.Journal of Investigative Dermatology,2015,135(6):1556.
[20]王修勇,刁庆春.Treg细胞与特应性皮炎的相关性研究进展[J].皮肤性病诊疗学杂志,2013,20(3):224.
[21]Cosmi L,Liotta F,Angeli R,et al.Th2cells are less susceptible than Th1cells to the suppressive activity of CD25+regulatory thymocytes because of their responsiveness to different cytokines[J].Blood,2004,103(8):3117.
[22]Ma L,Xue H B,Guan X H,et al.The Imbalance of Th17cells and CD4(+)CD25(high)Foxp3(+)Treg cells in patients with atopic dermatitis[J].Journal of the European Academy of Dermatology&Venereology,2014,28(8):1079.
[23]Ou L S,Goleva E,Hall C,et al.T regulatory cells in atopic dermatitis and subversion of their activity by superantigens[J].Journal of Allergy&Clinical Immunology,2004,113(4):756.
[2]Cosmi L,Liotta F,Angeli R,et al.Th2cells are less susceptible than Th1cells to the suppressive activity of CD25+regulatory thymocytes because of their responsiveness to different cytokines[J].Blood,2004,103(8):3117.
[3]中华医学会皮肤性病学分会免疫学组.中国特应性皮炎诊疗指南(2014版)[J].全科医学临床与教育,2014,47(6):603.
[4]彭星,刘玉梅.特应性皮炎发病机制的研究进展[J].中国实验诊断学,2014,18(10):1734.
[5]Brunner P M,Suárez-Fari1as M,He H,et al.The atopic dermatitis blood signature is characterized by increases in inflammatory and cardiovascular risk proteins[J].Sci Rep,2017,7(1):8707.
[6]Leung D Y,Boguniewicz M,Howell M D,et al.New insights into atopic dermatitis[J].Journal of Clinical Investigation,2004,113(5):651.
[7]Feng X,Yan S,Fei L,et al.Prevalence of Childhood Atopic Dermatitis:An Urban and Rural Community-Based Study in Shanghai,China[J].Plos One,2012,7(5):e36174.
[8]顾恒,颜艳,陈崑.我国特应性皮炎流行病学调查[J].中华皮肤科杂志,2000,33(6):379.
[9]Han K K.Overview of atopic dermatitis[J].Asia Pacific Allergy,2013,3(2):79.
[10]Brown S J,Irvine A D.Atopic Eczema and the Filaggrin Story[J].Seminars in Cutaneous Medicine&Surgery,2008,27(2):128.
[11]罗金成,宋志强.特应性皮炎的发病机制[J].中华临床免疫和变态反应杂志,2017,11(4):375.
[12]Kuo I,Carpentermendini A,Yoshida T,et al.Activation of epidermal toll-like receptor 2enhances tight junction function-Implications for atopic dermatitis and skin barrier repair[J].Journal of Investigative Dermatology,2013,133(4):988.
[13]Fujita H,Nograles K E,Kikuchi T,et al.Human Langerhans cells induce distinct IL-22-producing CD4+T cells lacking IL-17production[J].Proc Natl Acad Sci U S A,2009,106(51):21795.
[14]Mu Z,Zhao Y,Liu X,et al.Molecular Biology of Atopic Dermatitis[J].Clinical Reviews in Allergy&Immunology,2014,47(2):193.
[15]Cavani A,Pennino D,Eyerich K.Th17and Th22in Skin Allergy[J].Chemical Immunology&Allergy,2012,96(96):39.
[16]Auriemma M,Vianale G,Amerio P,et al.Cytokines and T cells in atopic dermatitis[J].European Cytokine Network,2013,24(1):37.
[17]Fujita H.Role of IL-22in the pathogenesis of skin diseases[J].Nihon Rinsho Meneki Gakkai Kaishi,2012,35(3):168.
[18]任小丽,苏湘川,石青青,等.Th22细胞及IL-22在特应性皮炎患者外周血中的表达及意义[J].中国中西医结合皮肤性病学杂志,2017,16(2):100.
[19]SangChul Han,DongHwan Koo,NaJin Kang,et al.Docosahexaenoic Acid Alleviates Atopic Dermatitis by Generating Treg and IL-10|[sol]|TGF-|[beta]|-Modified Macrophage via TGF-|[beta]|[J].Dependent Mechanism.Journal of Investigative Dermatology,2015,135(6):1556.
[20]王修勇,刁庆春.Treg细胞与特应性皮炎的相关性研究进展[J].皮肤性病诊疗学杂志,2013,20(3):224.
[21]Cosmi L,Liotta F,Angeli R,et al.Th2cells are less susceptible than Th1cells to the suppressive activity of CD25+regulatory thymocytes because of their responsiveness to different cytokines[J].Blood,2004,103(8):3117.
[22]Ma L,Xue H B,Guan X H,et al.The Imbalance of Th17cells and CD4(+)CD25(high)Foxp3(+)Treg cells in patients with atopic dermatitis[J].Journal of the European Academy of Dermatology&Venereology,2014,28(8):1079.
[23]Ou L S,Goleva E,Hall C,et al.T regulatory cells in atopic dermatitis and subversion of their activity by superantigens[J].Journal of Allergy&Clinical Immunology,2004,113(4):756.