便携式激光诱导荧光遥测系统研发与测试
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摘要
激光诱导荧光技术是水体叶绿素、有机物、微生物、溢油污染等快速测量的重要手段,对于大面积水域的测量,荧光遥测系统具有较大的优势。为了解决常规荧光测量系统体积巨大,不方便移动,测量不够灵活的问题,设计了一种小型化、便携式的测量系统,采用小型连续激光器、微型光谱仪、卡塞格林光学接收系统、激光测距仪、光纤和滤光片,对激光诱导荧光遥测系统的激光发射光路和收集光路进行设计和调试,通过对环境背景光的扣除实现对不同光照条件下的测量,并以水中小球藻的荧光信号测量进行系统验证。结果表明,本系统能够准确对测量点定位,实现水中藻类荧光的远程探测,并根据荧光强度和距离,计算水中藻体浓度。
Laser induced fluorescence(LIF) technology is an important means for the rapid measurement of chlorophyll,organic matter,microorganisms and oil spill pollution in water. For the measurement of large area of water,the fluorescence telemetry system has great advantages. Conventional fluorescence measurement system is huge,inconvenient to move and inflexible to measure. In order to solve these problems,a miniaturized and portable measurement system was designed. The system included a small continuous laser,a miniature spectrometer,a Cassegrain optical receiving system,a laser rangefinder,optical fibers and filters. It designed and debugged the emitting and collecting light paths of laser-induced fluorescence. Measurements under different illumination conditions were realized by deducting the background light. The system was validated by the fluorescence signal measurement of chlorella in water. The results show that the system can accurately locate the measuring points,realize the remote detection of algae fluorescence,and calculate the chlorophyll concentration in water according to the fluorescence intensity and distance.
Laser induced fluorescence(LIF) technology is an important means for the rapid measurement of chlorophyll,organic matter,microorganisms and oil spill pollution in water. For the measurement of large area of water,the fluorescence telemetry system has great advantages. Conventional fluorescence measurement system is huge,inconvenient to move and inflexible to measure. In order to solve these problems,a miniaturized and portable measurement system was designed. The system included a small continuous laser,a miniature spectrometer,a Cassegrain optical receiving system,a laser rangefinder,optical fibers and filters. It designed and debugged the emitting and collecting light paths of laser-induced fluorescence. Measurements under different illumination conditions were realized by deducting the background light. The system was validated by the fluorescence signal measurement of chlorella in water. The results show that the system can accurately locate the measuring points,realize the remote detection of algae fluorescence,and calculate the chlorophyll concentration in water according to the fluorescence intensity and distance.
引文
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[2]张石锐,董大明,郑文刚,等.农田土壤水分含量的激光诱导荧光光谱表征[J].光谱学与光谱分析,2012,32(10):2623-2627.
[3]来文豪,周孟然,王亚,等.深度学习与激光诱导荧光在假酒识别中的应用[J].激光与光电子学进展,2018(4):382-388.
[4]YASUNORI S,KENGO T,FUMITOSHI K,et al.Development of a UV laser-induced fluorescence lidar for monitoring blue-green algae in Lake Suwa[J].Appl Opt,2014,53(30):7030-7036.
[5]GIROD M,ARMSTRONG D W.Monitoring the migration behavior of living microorganisms in capillary electrophoresis using laser-induced fluorescence detection with a charge-coupled device imaging system[J].Electrophoresis,2015,23(13):2048-2056.
[6]韩晓爽,刘德庆,栾晓宁,等.基于激光诱导时间分辨荧光的原油识别方法研究[J].光谱学与光谱分析,2016,36(2):445-448.
[7]栾晓宁,张锋,郭金家,等.模拟溢油样品激光诱导荧光光谱的偏振特性研究[J].光谱学与光谱分析,2017,37(7):2092-2099.
[8]刘晓华,陈思颖,张寅超,等.基于激光诱导荧光的常见机油快速识别方法[J].光谱学与光谱分析.2014,34(8):2148-2151.
[9]金海龙,王玉田.基于荧光法的活体海藻识别方法研究[J].光学技术,2006,32(4):581-583.
[10]陈方,张晓燕,黄平捷,等.饮用水有机污染物的三维荧光光谱检测与分析方法[J].浙江大学学报:农业与生命科学版,2016,42(3):368-377.
[11]武晓莉,李艳君,吴铁军.基于选择性模型组合的三维荧光光谱水质分析方法[J].光谱学与光谱分析,2010,30(4):996-1001.
[12]BAZZANI M,CECCHI G,PANTANI L,et al.Venice Lagoon monitoring by a fluorescence lidar[C].International Geoscience&Remote Sensing Symposium,2002,3:1735-1737 vol.32002.
[13]WANG LIN,QIU ZHONGFENG,PANG HUIFANG,et al.Chlorophyll Fluorescence Extraction from Water-Leaving Radiance of Algae-Containing Water Through Polarization[J].Journal of Ocean University of China,2017,16(6):1003-1008.