基质浓度对分体式厌氧氨氧化工艺固碳能力的影响
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摘要
采用分体式厌氧氨氧化反应装置,以短程硝化-厌氧氨氧化工艺为研究对象,通过改变基质浓度探究其对工艺固碳潜力的影响,寻找最佳固碳量的运行工况;通过收集反应器运行各阶段的污泥样品,对其中的微生物种群多样性进行分析,确定工艺的固碳微生物。试验结果表明:确定进水中NH~+_4-N浓度为180 mg/L时,工艺展现出最佳的固碳能力;短程硝化阶段,当进水中NLR为0.44 kg-N/(m~3·d~(-1))、HRT为10 h时,固碳量为0.285 mg/mg-N;厌氧氨氧化阶段,当进水中NH~+_4-N和NO~-_2-N浓度分别为75 mg/L、95 mg/L,HRT为24 h、NLR为0.13 kg-N/(m~3·d~(-1))时,固碳量为0.16 mg/mg-N。微生物种群多样性分析表明:短程硝化反应器中的优势菌种为Acidobacteria Bacteria(酸杆菌)、Chlorobi(绿菌)、Proteobacteria Bacteria(变形杆菌);厌氧氨氧化反应器中的优势菌种为Planctomycete Bacteria(浮霉菌)、Actinobacteria Bacteria(放线菌)、Proteobacteria Bacteria(变形杆菌)。
This paper investigates the effect of substrate concentration on carbon sequestration capacity in a two-stage anammox process using partial nitrification reactor and anammox reactor.The paper also collects the sludge samples from each stage during the study,and analyzes the microbial population to determine the carbon-fixing microorganisms of the process.The results show that the process achieves the highest carbon sequestration capacity with the influent NH~+_4-N concentration of 180 mg/L.When the NLR is 0.44 kg-N/(m~3·d~(-1)) and the HRT is 10 h,the carbon sequestration of partial nitrification reactor is 0.285 mg/mg-N.In the anammox reactor,the carbon sequestration is 0.16 mg/mg-N with the influent NH~+_4-N concentration of 75 mg/L,influent NO~-_2-N concentration of 95 mg/L,HRT of 24 h,and NLR of 0.13 kg-N/(m~3·d~(-1)).Acidobacteria Bacteria,Chlorobi and Proteobacteria Bacteria are the predominant bacteria in partial nitrification reactor.Meanwhile,the predominant bacteria in anammox reactor are Planctomycete Bacteria,Actinobacteria Bacteria and Proteobacteria Bacteria.
This paper investigates the effect of substrate concentration on carbon sequestration capacity in a two-stage anammox process using partial nitrification reactor and anammox reactor.The paper also collects the sludge samples from each stage during the study,and analyzes the microbial population to determine the carbon-fixing microorganisms of the process.The results show that the process achieves the highest carbon sequestration capacity with the influent NH~+_4-N concentration of 180 mg/L.When the NLR is 0.44 kg-N/(m~3·d~(-1)) and the HRT is 10 h,the carbon sequestration of partial nitrification reactor is 0.285 mg/mg-N.In the anammox reactor,the carbon sequestration is 0.16 mg/mg-N with the influent NH~+_4-N concentration of 75 mg/L,influent NO~-_2-N concentration of 95 mg/L,HRT of 24 h,and NLR of 0.13 kg-N/(m~3·d~(-1)).Acidobacteria Bacteria,Chlorobi and Proteobacteria Bacteria are the predominant bacteria in partial nitrification reactor.Meanwhile,the predominant bacteria in anammox reactor are Planctomycete Bacteria,Actinobacteria Bacteria and Proteobacteria Bacteria.
引文
[1] Chamchoi N,Nitisoravut S,Schmidt J E.Inactivation of anammox communities under concurrent operation of anaerobic ammonium oxidation (ANAMMOX) and denitrification[J].Bioresource Technology,2008,99(9):3331-3336.
[2] Ni S Q,Lee P,Fessehaie A,et al.Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor[J].Bioresource Technology,2010,101(6):1792-1799.
[3] Liu J,Zuo J,Yang Y,et al.An autotrophic nitrogen removal process:Short-cut nitrification combined with ANAMMOX for treating diluted effluent from an UASB reactor fed by landfill leachate[J].Journal of Environmental Sciences,2010,22(5):777-783.
[4] Sri Shalini S,Joseph K.Nitrogen management in landfill leachate:Application of SHARON,ANAMMOX and combined SHARON-ANAMMOX process[J].Waste Management,2012,32(12):2385-2400.
[5] Strous M,Van Gerven E,Zheng P,et al.Ammonium removal from concentrated waste streams with the anaerobic ammonium oxidation (ANAMMOX) process in different reactor configurations[J].Water Research,1997,31(8):1955-1962.
[6] Stein L Y,Yung Y L.Production,isotopic composition,and atmospheric fate of biologically produced nitrous oxide[J].Annual Review of Earth & Planetary Sciences,2003,31(31):329-356.
[7] 马富国.部分亚硝化-厌氧氨氧化耦合工艺处理污泥脱水液的研究[D].北京:北京工业大学,2009.
[8] 谭发,王敦球,金樾,等.有机碳源对一体式厌氧氨氧化反应器脱氮性能的影响[J].安全与环境工程,2016,23(4):44-50.
[9] Muller E B,Stouthamer A H,van Verseveld H W.Simultaneous NH3 oxidation and N2 production at reduced O2 tensions by sewage sludge subcultured with chemolithotrophic medium[J].Bio degradation,1995,6:339-349.
[10]Hellinga C,Schellen A A J C,Mulder J W,et al.The sharon process:An innovative method for nitrogen removal from ammonium-rich waste water[J].Water Science & Technology,1998,37(9):135-142.
[11]李泽兵,刘常敬,赵白航,等.多基质时厌氧氨氧化菌、异养反硝化污泥活性及抑制特征[J].中国环境科学,2013,33(4):648-654.
[12]Tao W,He Y,Wang Z,et al.Effects of pH and temperature on coupling nitritation and anammox in biofilters treating dairy wastewater[J].Ecological Engineering,2012,47:76-82.
[13]Chouten S,Strous M,Kuypers MMM,et al.Stable carbon isotopic fractionations associated with inorganic carbon fixation by anaerobic ammonium-oxidizing bacteria[J].Applied and Environmental Microbiology,2004,70(6):3785-3788.
[14]Imura Y,Isaka K,Kazama F.Effects of inorganic carbon limitation on anaerobic ammonium oxidation (anammox) activity[J].Bioresource Technology,2011,102(6),4390-4394.
[15]Strous M,Kuenen J G,Jetten M S M.Key physiology of anaerobic ammonium oxidation[J].Applied and Environmental Microbiology,1999,65(7):3248-3250.
[16]Woebken D,Lam P,Kuypers M M M,et al.A microdiversity study of anammox bacteria reveals a novel Candidatus Scalindua phylotype in marine oxygen minimum zones[J].Environmental Microbiology,2008.10(11):3106-3119.
[17]李亮,王敦球,农秋悦,等.PNH工艺处理高浓度氨氮废水短程硝化试验研究[J].安全与环境工程,2013,20(6):85-89.
[18]Zhang W J,Zhang Y Y,Li L,et al.Fast start-up of expanded granular sludge bed (EGSB) reactor using stored Anammox sludge[J].Water Science & Technology,2014,69(7):1469-1474.
[19]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[20]Bassham J A,Benson A A,Calvin M.The path of carbon in photosynthesis.VIII.The role of malic acid[J].Journal of Biological Chemistry,1950,185(2):781-787.
[21]杜兵,司亚安,孙艳玲,等.推流固定化生物反应器培养ANAMMOX菌[J].中国给水排水,2003,19(7):62-65.
[22]袁红朝,秦红灵,刘守龙,等.固碳微生物分子生态学研究[J].中国农业科学,2011,44(14):2951-2958.
[23]郝晓地.可持续污水-废物处理技术[M].北京:中国建筑工业出版社,2006.
[2] Ni S Q,Lee P,Fessehaie A,et al.Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor[J].Bioresource Technology,2010,101(6):1792-1799.
[3] Liu J,Zuo J,Yang Y,et al.An autotrophic nitrogen removal process:Short-cut nitrification combined with ANAMMOX for treating diluted effluent from an UASB reactor fed by landfill leachate[J].Journal of Environmental Sciences,2010,22(5):777-783.
[4] Sri Shalini S,Joseph K.Nitrogen management in landfill leachate:Application of SHARON,ANAMMOX and combined SHARON-ANAMMOX process[J].Waste Management,2012,32(12):2385-2400.
[5] Strous M,Van Gerven E,Zheng P,et al.Ammonium removal from concentrated waste streams with the anaerobic ammonium oxidation (ANAMMOX) process in different reactor configurations[J].Water Research,1997,31(8):1955-1962.
[6] Stein L Y,Yung Y L.Production,isotopic composition,and atmospheric fate of biologically produced nitrous oxide[J].Annual Review of Earth & Planetary Sciences,2003,31(31):329-356.
[7] 马富国.部分亚硝化-厌氧氨氧化耦合工艺处理污泥脱水液的研究[D].北京:北京工业大学,2009.
[8] 谭发,王敦球,金樾,等.有机碳源对一体式厌氧氨氧化反应器脱氮性能的影响[J].安全与环境工程,2016,23(4):44-50.
[9] Muller E B,Stouthamer A H,van Verseveld H W.Simultaneous NH3 oxidation and N2 production at reduced O2 tensions by sewage sludge subcultured with chemolithotrophic medium[J].Bio degradation,1995,6:339-349.
[10]Hellinga C,Schellen A A J C,Mulder J W,et al.The sharon process:An innovative method for nitrogen removal from ammonium-rich waste water[J].Water Science & Technology,1998,37(9):135-142.
[11]李泽兵,刘常敬,赵白航,等.多基质时厌氧氨氧化菌、异养反硝化污泥活性及抑制特征[J].中国环境科学,2013,33(4):648-654.
[12]Tao W,He Y,Wang Z,et al.Effects of pH and temperature on coupling nitritation and anammox in biofilters treating dairy wastewater[J].Ecological Engineering,2012,47:76-82.
[13]Chouten S,Strous M,Kuypers MMM,et al.Stable carbon isotopic fractionations associated with inorganic carbon fixation by anaerobic ammonium-oxidizing bacteria[J].Applied and Environmental Microbiology,2004,70(6):3785-3788.
[14]Imura Y,Isaka K,Kazama F.Effects of inorganic carbon limitation on anaerobic ammonium oxidation (anammox) activity[J].Bioresource Technology,2011,102(6),4390-4394.
[15]Strous M,Kuenen J G,Jetten M S M.Key physiology of anaerobic ammonium oxidation[J].Applied and Environmental Microbiology,1999,65(7):3248-3250.
[16]Woebken D,Lam P,Kuypers M M M,et al.A microdiversity study of anammox bacteria reveals a novel Candidatus Scalindua phylotype in marine oxygen minimum zones[J].Environmental Microbiology,2008.10(11):3106-3119.
[17]李亮,王敦球,农秋悦,等.PNH工艺处理高浓度氨氮废水短程硝化试验研究[J].安全与环境工程,2013,20(6):85-89.
[18]Zhang W J,Zhang Y Y,Li L,et al.Fast start-up of expanded granular sludge bed (EGSB) reactor using stored Anammox sludge[J].Water Science & Technology,2014,69(7):1469-1474.
[19]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[20]Bassham J A,Benson A A,Calvin M.The path of carbon in photosynthesis.VIII.The role of malic acid[J].Journal of Biological Chemistry,1950,185(2):781-787.
[21]杜兵,司亚安,孙艳玲,等.推流固定化生物反应器培养ANAMMOX菌[J].中国给水排水,2003,19(7):62-65.
[22]袁红朝,秦红灵,刘守龙,等.固碳微生物分子生态学研究[J].中国农业科学,2011,44(14):2951-2958.
[23]郝晓地.可持续污水-废物处理技术[M].北京:中国建筑工业出版社,2006.