铜离子浓度对嗜酸性氧化亚铁硫杆菌生长特性和生长动力学的影响
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摘要
通过研究Cu~(2+)浓度对嗜酸性氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans,A.f)生长特性的影响,得到在不同Cu~(2+)浓度下A.f菌的生长曲线,探究不同Cu~(2+)浓度对培养液中细菌浓度、p H、亚铁浓度和氧化还原电位的影响。结果表明:A.f菌对Cu~(2+)具有一定的耐受能力;当培养液中ρ(Cu~(2+))≤0.5 g/L时,Cu~(2+)对A.f菌的生长活性影响较小;当ρ(Cu~(2+))在1.0~2.0 g/L之间变化时,A.f菌的生长活性开始受到Cu~(2+)的影响,出现明显的延迟效应,Cu~(2+)开始抑制细菌生长繁殖;当ρ(Cu~(2+))≥3.5 g/L时,A.f菌生长完全受到抑制,几乎停止生长。随着培养液中Cu~(2+)浓度的增加,A.f菌生长活性受到的抑制作用也增大。依据Monod方程推导并建立了A.f菌在不同Cu~(2+)浓度下的生长动力学方程,各动力学曲线的相关性较好,表明动力学方程能较好地描述不同Cu~(2+)浓度对氧化亚铁硫杆菌生长的影响。
The effect of initial copper ion concentration on the growth activity of Acidithiobacillus ferrooxidans was investigated, and the bacterial growth curves, bacteria concentration, p H, ferrous concentration and oxidation-reduction potential in the culture solution were obtained at different Cu~(2+) concentrations. The results show that to some extent Acidithiobacillus ferrooxidans is tolerant to copper ion. When cultured in 9K medium containing ρ(Cu~(2+))≤0.5 g/L, little effect on the growth and activity of Acidithiobacillus ferrooxidans is brought to copper ion. When ρ(Cu~(2+)) is 1.0-2.0 g/L, the growth and activity of Acidithiobacillus ferrooxidans are obviously influenced by copper ion and appear delayed effects, and then copper iron begins to inhibit the bacterial growth. When the concentration of copper ion is over 3.5 g/L, the inhibiting effect on activity of Acidithiobacillus ferrooxidans is completely inhibited, bacteria almost stops growing. With the increase of Cu~(2+) concentration in the culture medium, the inhibiting effect on growth activity of Acidithiobacillus ferrooxidans increases. Based on the model of Monod function, the growth kinetics equation of Acidithiobacillus ferrooxidans at different copper iron concentration is built up. The correlation of kinetic curve is better, which shows that the kinetics equation can well describe the growth of Acidithiobacillus ferrooxidans at different initial copper iron concentrations.
The effect of initial copper ion concentration on the growth activity of Acidithiobacillus ferrooxidans was investigated, and the bacterial growth curves, bacteria concentration, p H, ferrous concentration and oxidation-reduction potential in the culture solution were obtained at different Cu~(2+) concentrations. The results show that to some extent Acidithiobacillus ferrooxidans is tolerant to copper ion. When cultured in 9K medium containing ρ(Cu~(2+))≤0.5 g/L, little effect on the growth and activity of Acidithiobacillus ferrooxidans is brought to copper ion. When ρ(Cu~(2+)) is 1.0-2.0 g/L, the growth and activity of Acidithiobacillus ferrooxidans are obviously influenced by copper ion and appear delayed effects, and then copper iron begins to inhibit the bacterial growth. When the concentration of copper ion is over 3.5 g/L, the inhibiting effect on activity of Acidithiobacillus ferrooxidans is completely inhibited, bacteria almost stops growing. With the increase of Cu~(2+) concentration in the culture medium, the inhibiting effect on growth activity of Acidithiobacillus ferrooxidans increases. Based on the model of Monod function, the growth kinetics equation of Acidithiobacillus ferrooxidans at different copper iron concentration is built up. The correlation of kinetic curve is better, which shows that the kinetics equation can well describe the growth of Acidithiobacillus ferrooxidans at different initial copper iron concentrations.
引文
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[2]刘欣伟,胡文韬,李浩然,冯雅丽.高镁型低品位硫化镍矿浸出工艺及机理[J].中国有色金属学报,2014,24(6):1599-1606.LIU Xin-wei,HU Wen-tao,LI Hao-ran,FENG Ya-li.Leaching process and mechanism of high magnesium low-grade nickel sulfide ore[J].The Chinese Journal of Nonferrous Metals,2014,24(6):1599-1606.
[3]谢红艳,王吉坤,路辉,嵇晓沧,杨世诚.加压浸出低品位锰矿的工艺[J].中国有色金属学报,2013,23(6):1701-1711.XIE Hong-yan,WANG Ji-kun,LU Hui,JI Xiao-cang,YANG Shi-cheng.Technique of pressure leaching low grade manganese ore[J].The Chinese Journal of Nonferrous Metals,2013,23(6):1701-1711.
[4]LI Chang-xin,ZHONG Hong,WANG Shuai,XUE Jian-rong,WU Fang-fang,ZHANG Zhen-yu.Manganese extraction by reduction-acid leaching from low-grade manganese oxide ores using Ca S as reductant[J].Transactions of Nonferrous Metals Society of China,2015,25(4):1677-1684.
[5]ZHU Jian-yu,LI Qian,JIAO Wei-feng,JIANG Hao,SAND Wolfgang,XIA Jin-lan,LIU Xue-duan,QIN Wen-qing,QIU Guan-zhou,HU Yue-hua,CHAI Li-yuan.Adhesion forces between cells of Acidithiobacillus ferrooxidans,Acidithiobacillus thiooxidans or Leptospirillum ferrooxidans and chalcopyrite[J].Colloids and Surfaces B,2012,94:95-100.
[6]CHEN Shen-yi,LIN Jih-gaw.Enhancement of metal bioleaching from contaminated sediment using silver ion[J].Journal of Hazardous Materials,2009,161:893-899.
[7]MOGHADDAM M Y,SHAFAEI S Z,NOAPARAST M,ARDEJANI F D,ABDOLLAHI H,RANJBAR M,SCHAFFIE M,MANAFI Z.Empirical model for bio-extraction of copper from low grade ore using response surface methodology[J].Transactions of Nonferrous Metals Society of China,2015,25(12):4126-4143.
[8]郝晓东,曾伟民,彭堂见,胡琪,梁伊丽,尹华群,邱冠周,刘学端.高通量测序技术分析不同温度下赞比亚低品位铜矿生物浸出过程中的微生物多样性[J].中国有色金属学报,2015,25(9):2558-2564.HAO Xiao-dong,ZENG Wei-min,PENG Tang-jian,HU Qi,LIANG Yi-li,YIN Hua-qun,QIU Guan-zhou,LIU Xue-duan.Analysis of microbial diversity during bioleaching of low grade copper ore from Zambia using high-throughput sequencing technology at different temperatures[J].The Chinese Journal of Nonferrous Metals,2015,25(9):2558-2564.
[9]顾帼华,陈明莲,苏丽君,李建华,胡岳华,邱冠周.氧化亚铁硫杆菌对黄铜矿表面性质及其浸出的影响[J].中南大学学报(自然科学版),2010,41(3):807-812.GU Guo-hua,CHEN Ming-lian,SU Li-jun,LI Jian-hua,HU Yue-hua,QIU Guan-zhou.Effects of Acidithiobacillus ferrooxidans on surface properties of chalcopyrite and bioleaching[J].Journal of Central South University(Science and Technology),2010,41(3):807-812.
[10]王进龙,王军.国内某露天剥离低品位铜矿石的生物柱浸研究[J].矿产保护与利用,2015,6:27-30.WANG Jin-long,WANG Jun.Experimental research on column bioleaching of a low grade copper ore of an open pit in China[J].Conservation and Utilization of Mineral Resources,2015,6:27-30.
[11]PRADHAN N,NATHSARMA K C,RAO K S,SUKLA L B,MISHRA B K.Heap bioleaching of chalcopyrite:A review[J].Minerals Engineering,2008,21:355-365.
[12]喻连香,周丹,石丽娟,王玉光,曾伟民,邱冠周.国内某低品位难选铜矿的生物浸出研究[J].现代生物医学进展,2014,14(10):1861-1864.YU Lian-xiang,ZHOU Dan,SHI Li-juan,WANG Yu-guang,ZENG Wei-min,QIU Guan-zhou.Bioleaching of some low grade refractory copper ore[J].Progress in Modern Biomedicine,2014,14(10):1861-1864.
[13]谢鑫源,孙培德,楼菊青,郭茂新,马王钢.模拟电镀污泥重金属浸出液对氧化亚铁硫杆菌活性的影响[J].环境科学,2013,34(1):209-216.XIE Xin-yuan,SUN Pei-de,LOU Ju-qing,GUO Mao-xin,MA Wang-gang.Effect of simulated heavy metal leaching solution of electroplating sludge on the bioactivity of Acidithiobacillus ferrooxidans[J].Environmental Science,2013,34(1):209-216.
[14]赵雪淞,石倩倩,李彩霞,张孝松.镍离子对氧化亚铁微螺菌和喜温嗜酸硫杆菌活性的影响[J].硅酸盐通报,2016,35(1):306-309.ZHAO Xue-song,SHI Qian-qian,LI Cai-xia,ZHANG Xiao-song.Effect of nickel ions on activity of Leptospirillums thermoferrooxidans and Acidithiobacillus caldus[J].Bulletin of the Chinese Ceramic Society,2016,35(1):306-309.
[15]刘欣伟,冯雅丽,李浩然,蔡震雷,杨志超.镁离子浓度对氧化亚铁硫杆菌生长动力学的影响[J].中国有色金属学报,2012,22(8):2353-2359.LIU Xin-wei,FENG Ya-li,LI Hao-ran,CAI Zhen-lei,YANG Zhi-chao.Effect of magnesium ion concentration on growth kinetics of Thiobacillus ferroxidans[J].The Chinese Journal of Nonferrous Metals,2012,22(8):2353-2359.
[16]汪智姝,陈晓,李万全,徐绍霞,张永奎.高铁浓度下氧化亚铁硫杆菌的生长动力学[J].化工进展,2007,26(10):1475-1478.WANG Zhi-shu,CHEN Xiao,LI Wan-quan,XU Shao-xia,ZHANG Yong-kui.Growth kinetics of Thiobacillus ferrooxidans at high iron concentration[J].Chemical Industry and Engineering Progress,2007,26(10):1475-1478.
[17]CABRERA G,GOMEZ J M,CANTERO D.Kinetic study of ferrous sulphate oxidation of Acidithiobacillus ferrooxidans in the presence of heavy metal ions[J].Enzyme and Microbial Technology,2005,36:301-306.
[18]马骏,汪菊香,武彪,温建康,尚鹤,武名麟.晶体结构对黄铜矿、黄铁矿生物浸出差异性影响[J].中国有色金属学报,2015,25(10):2898-2904.MA Jun,WANG Ju-xiang,WU Biao,WEN Jian-kang,SHANG He,WU Ming-lin.Effects of crystal structure on differences of chalcopyrite and pyrite bioleaching[J].The Chinese Journal of Nonferrous Metals,2015,25(10):2898-2904.
[19]聂红燕,朱能武,杨婷婷,张嘉妮.嗜酸性细菌对废旧线路板浸出的吸附行为及动力学[J].环境科学学报,2015,35(5):1471-1476.NIE Hong-yan,ZHU Neng-wu,YANG Ting-ting,ZHANG Jia-ni.Adsorption behavior and kinetics of adcidophilic bacteria on the leaching of waste printed circuit boards[J].Acta Scientiae Circumstantiae,2015,35(5):1471-1476.
[20]NGUYEN V K,LEE M H,PARK H J,LEE J U.Bioleaching of arsenic and heavy metals from mine tailings by pure and mixed cultures of Acidithiobacillus spp.[J].Journal of Industrial and Engineering Chemistry,2015,21:451-458.
[21]国家环境保护总局,《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002:368-370.State Environmental Protection Administration,Editorial Committee of Analysis in Water and Wastewater.Analysis in water and wastewater[M].4th ed.Beijing:China Environmental Science Press,2002:368-370.
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