耳针对氧化偶氮甲烷致直肠癌大鼠食欲的影响及其机制研究
|
摘要
目的探讨耳针对氧化偶氮甲烷(AOM)致直肠癌大鼠食欲的影响及其机制研究。方法用AOM腹腔注射诱导大鼠发生直肠癌,从造模开始,每周对耳针组、体针组大鼠进行针刺治疗,观察耳针对大鼠体重、摄食量、血清食欲素A、瘦素、胸腺指数、脾脏指数以及直肠病理组织学的影响。结果用AOM成功诱导了大鼠直肠癌模型,正常对照组未见肿瘤发生,模型组、耳针组、体针组各组大鼠直肠部分均不同程度出现肿瘤;与模型组比较,第10、15、20周体针组、耳针组体重明显增加,其中第15周体针组、耳针组体重显著增加(P <0. 05);与模型组比较,第5、10、15、20周体针组、耳针组大鼠摄食量明显增加,其中第5周体针组、耳针组摄食量显著增加(P <0. 05,P <0. 01);与正常组比较,模型组大鼠血清食欲素A、瘦素均显著升高(P <0. 01);与模型组比较,耳针组、体针组大鼠血清食欲素A、瘦素均显著降低(P <0. 01);与正常组比较,模型组、耳针组、体针组脾脏指数均有升高趋势,胸腺指数均有下降趋势;但各组大鼠脾脏指数、胸腺指数变化差异均无统计学意义。结论耳针治疗可以明显增强AOM致直肠癌大鼠摄食量,可能与耳针刺激使得血清中廋素、食欲素A含量变化有关。
Objective To explore the effect and mechanism of auricular acupuncture on appetite in the rats of rectal cancer induced by azoxymethane( AOM). Methods The intraperitoneal injection of AOM was used to induce rectal cancer in rats. Since modeling,acupuncture was given in the auricular acupuncture group and the body acupuncture group.The body mass,food intake,serum orexin A,leptin,thymus index,spleen index and the rectal historical effects were observed in the treatment with auricular acupuncture. Results In the research,the rectal cancer models were successfully induced with AOM in the rats. No tumor was found in the normal control group. Tumors were discovered in the rectum of the rats in each of the model group,the auricular acupuncture group and the body acupuncture group. Compared with the model group,in the week 10,15 and 20,the body masses were increased obviously in the body acupuncture group and the auricular acupuncture group,in which,the body mass was increased significantly in the week 15( P < 0. 05). Compared with the model group,in the week 10,15 and 20,the food intake was increased obviously in the body acupuncture group and the auricular acupuncture group,in which,in the week 5,the food intake was increased significantly( P < 0. 05,P < 0. 01). Compared with the normal group,serum orexin A and leptin were increased significantly in the model group( P < 0. 01). Compared with the model group,they were reduced significantly in the body acupuncture group and the auricular acupuncture group( P < 0. 01). Compared with the normal group,the spleen indexes were all increased in tendency in the model group,the body acupuncture group and the auricular acupuncture group and the thymus indexes were all reduced in tendency. The differences were not significant in spleen index and thymus index. Conclusion The auricular acupuncture remarkably increases the food intake in the rats of AOM-induced rectal cancer,which is probably related to the changes of serum leptin and orexin A after the stimulation with auricular acupuncture.
Objective To explore the effect and mechanism of auricular acupuncture on appetite in the rats of rectal cancer induced by azoxymethane( AOM). Methods The intraperitoneal injection of AOM was used to induce rectal cancer in rats. Since modeling,acupuncture was given in the auricular acupuncture group and the body acupuncture group.The body mass,food intake,serum orexin A,leptin,thymus index,spleen index and the rectal historical effects were observed in the treatment with auricular acupuncture. Results In the research,the rectal cancer models were successfully induced with AOM in the rats. No tumor was found in the normal control group. Tumors were discovered in the rectum of the rats in each of the model group,the auricular acupuncture group and the body acupuncture group. Compared with the model group,in the week 10,15 and 20,the body masses were increased obviously in the body acupuncture group and the auricular acupuncture group,in which,the body mass was increased significantly in the week 15( P < 0. 05). Compared with the model group,in the week 10,15 and 20,the food intake was increased obviously in the body acupuncture group and the auricular acupuncture group,in which,in the week 5,the food intake was increased significantly( P < 0. 05,P < 0. 01). Compared with the normal group,serum orexin A and leptin were increased significantly in the model group( P < 0. 01). Compared with the model group,they were reduced significantly in the body acupuncture group and the auricular acupuncture group( P < 0. 01). Compared with the normal group,the spleen indexes were all increased in tendency in the model group,the body acupuncture group and the auricular acupuncture group and the thymus indexes were all reduced in tendency. The differences were not significant in spleen index and thymus index. Conclusion The auricular acupuncture remarkably increases the food intake in the rats of AOM-induced rectal cancer,which is probably related to the changes of serum leptin and orexin A after the stimulation with auricular acupuncture.
引文
[1]毕小彬,汪宇红.醋酸甲地孕酮与癌性厌食-恶病质[J].上海医药,2008,29(10):474-475.
[2]李培训,贾英杰.针刺治疗晚期消化道肿瘤厌食症27例疗效观察[J].新中医,2006,38(4):67-68.
[3]周静珠,王茵萍,仲远明.耳针疗法治疗消化系统疾病的临床研究[J].针灸临床杂志,2011,27(7):54-56.
[4]Lee MS,Shin BC. More about auricular acupuncture for insomnia[J]. J Altern Complement Med,2008,14(2):106-108.
[5]Ouyang H,Chen JD. Review article:therapeutic roles of acupuncture in functional gastrointestinal disorders[J]. Aliment Pharmacol Ther,2004,20(8):831-841.
[6]Ouyang H,Yin J,Wang Z,et al. Electroacupuncture accelerates gastric emptying in association with changes in vagal activity[J]. Am J Physiol Gastrointest Liver Physiol,2002,282(2):G390-G396.
[7]贺婵婵,张蓉,张华,等.食欲素与肿瘤关系的研究进展[J].中国全科医学,2016,19(8):986-988.
[8]Willie JT,Chemelli RM,Sinton CM,et al. To eat or to sleep Orexin in the regulation of feeding and wakefulness[J]. Annu Rev Neurosci,2001,24:429-458.
[9]刘兵兵,杜红珍,李增宁,等.食欲素功能研究及在肿瘤治疗方面应用的前景[J].肿瘤代谢与营养电子杂志,2015,2(3):64-67.
[10]Isse N,Ogawa Y,Tamura N,et al. Structural Organization and Chromosomal Assignment of the Human Obese Gene[J]. J Biol Chem,1995,270(46):27728-27733.
[11]尚晓宇,阳学风.瘦素与肿瘤的关系[J].国际病理科学与临床杂志,2008,28(5):416-420.
[2]李培训,贾英杰.针刺治疗晚期消化道肿瘤厌食症27例疗效观察[J].新中医,2006,38(4):67-68.
[3]周静珠,王茵萍,仲远明.耳针疗法治疗消化系统疾病的临床研究[J].针灸临床杂志,2011,27(7):54-56.
[4]Lee MS,Shin BC. More about auricular acupuncture for insomnia[J]. J Altern Complement Med,2008,14(2):106-108.
[5]Ouyang H,Chen JD. Review article:therapeutic roles of acupuncture in functional gastrointestinal disorders[J]. Aliment Pharmacol Ther,2004,20(8):831-841.
[6]Ouyang H,Yin J,Wang Z,et al. Electroacupuncture accelerates gastric emptying in association with changes in vagal activity[J]. Am J Physiol Gastrointest Liver Physiol,2002,282(2):G390-G396.
[7]贺婵婵,张蓉,张华,等.食欲素与肿瘤关系的研究进展[J].中国全科医学,2016,19(8):986-988.
[8]Willie JT,Chemelli RM,Sinton CM,et al. To eat or to sleep Orexin in the regulation of feeding and wakefulness[J]. Annu Rev Neurosci,2001,24:429-458.
[9]刘兵兵,杜红珍,李增宁,等.食欲素功能研究及在肿瘤治疗方面应用的前景[J].肿瘤代谢与营养电子杂志,2015,2(3):64-67.
[10]Isse N,Ogawa Y,Tamura N,et al. Structural Organization and Chromosomal Assignment of the Human Obese Gene[J]. J Biol Chem,1995,270(46):27728-27733.
[11]尚晓宇,阳学风.瘦素与肿瘤的关系[J].国际病理科学与临床杂志,2008,28(5):416-420.