小鼠内耳发育过程中Evi-1基因的表达
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摘要
目的观察在小鼠内耳发育过程中不同阶段Evi-1基因的动态表达特征及变化规律,为进一步探索EVI-1基因在小鼠内耳发育中作用。方法选用胚胎第9.5天(E9.5)到胚胎期18.5天(E18.5)的C57BL/6胎鼠,E9.5-E15.5取胚胎头,E15.5后取内耳,之后进行冰冻切片及免疫荧光染色,利用荧光显微镜观察小鼠内耳发育过程中EVI-1基因的表达变化情况。结果在E9.5天时尚未在听泡及其周围组织观察到EVI-1基因的表达,当胚胎发育到E10.5天时,EVI-1开始在感觉上皮周围弱表达,其局限表达于听泡周围的间充质;E11.5时期Evi-1的表达增强但也局限于内耳周围的间充质;E12.5时期时感觉上皮中开始出现Evi-1的表达,但随后即消失,仅在这一时期的感觉上皮中一过性表达,E13.5后,Evi-1表达逐渐减弱至消失。结论 Evi-1基因在小鼠内耳发育时期的表达具有明显时限性以及区域性,可能与内耳感觉细胞的增殖分化有关。
Objective To report dynamic expression characteristics of the Evi-1 gene during different stages of inner ear development. Methods Embryonic C57 BL/6 mice ranging from E9.5 to E18.5 were used to observe Evi-1 gene expression during the inner ear development process on serial frozen sections with immunofluorescence staining under a fluorescent microscope. Result Otocyst aroused on E9.5 when there was no visible expression of Evi-1 in the inner ear.The expression of Evi-1 emerged on E10.5 and was localized in periotic mesenchyme during embryonic development from E10.5 to E13.5. Moreover, strong Evi-1 expression was seen not only in mesenchyme by E11.5 but also transiently on sensory epithelium on E12.5. Fluorescence signals gradually decreased after E13.5 and expression of Evi-1 gene in the inner ear and periotic mesenchyme disappeared by E14.5. Conclusion The temporary and regional expression of the Evi-1 gene during the development of the inner ear indicates that Evi-1 may have a significant function during the process of hair cells development.
Objective To report dynamic expression characteristics of the Evi-1 gene during different stages of inner ear development. Methods Embryonic C57 BL/6 mice ranging from E9.5 to E18.5 were used to observe Evi-1 gene expression during the inner ear development process on serial frozen sections with immunofluorescence staining under a fluorescent microscope. Result Otocyst aroused on E9.5 when there was no visible expression of Evi-1 in the inner ear.The expression of Evi-1 emerged on E10.5 and was localized in periotic mesenchyme during embryonic development from E10.5 to E13.5. Moreover, strong Evi-1 expression was seen not only in mesenchyme by E11.5 but also transiently on sensory epithelium on E12.5. Fluorescence signals gradually decreased after E13.5 and expression of Evi-1 gene in the inner ear and periotic mesenchyme disappeared by E14.5. Conclusion The temporary and regional expression of the Evi-1 gene during the development of the inner ear indicates that Evi-1 may have a significant function during the process of hair cells development.
引文
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14 Lang WJ,Chen FY.The reciprocal link between EVI1 and miR-NAs in human malignancies[J].Gene,2018.672:56-63.
15 Seale P,Bjork B,Yang W,et al.PRDM16 controls a brown fat/skeletal muscle switch[J].Nature,2008,454(7207):961-U27.
16 Moore AW,Jan LY,Jan YN.hamlet,a binary genetic switch between single-and multiple-dendrite neuron morphology[J].Science,2002,297(5585):1355-1358.
17 Alliston T,Ko TC,Cao Y,et al.Repression of Bone Morphogenetic Protein and Activin-inducible Transcription by Evi-1[J].Journal of Biological Chemistry,2005,280(25):24227-24237.
18 Dabdoub A,Puligilla C,Jones JM,et al.Sox2 signaling in prosensory domain specification and subsequent hair cell differentiation in the developing cochlea[J].Proceedings of the National Academy of Sciences of the United States of America,2008.105(47):18396-18401.
19 Hume CR,Bratt DL,Oesterle EC.Expression of LHX3 and SOX2 during mouse inner ear development[J].Gene Expression Patterns,2007.7(7):798-807.
20 Eguchi R,Yooshigai E,Koga T,et al.Spatiotemporal expression of Prdm genes during Xenopus development[J].Cytotechnology,2015,67(4):711-719.
21 Mead PE,Paraganas E,Ohtsuka S,et al.Evi-1 expression in Xenopus[J].Gene Expression Patterns,2005,5(5):601-608.
22刘少峰,李华伟,周梁.胚胎小鼠耳蜗感觉上皮的形态发生[J].中国耳鼻咽喉头颈外科.2005,12(7):433-436.Liu SF,Li HW,Zhou L.Morphogenesis in the sensory epithelia of embryonic mouse cochlea[J].Chinese Archives of Otolaryngology-head and Neck Surgery,2005,12(7):433-436).
23 Fuhrmann S,Levine EM,Reh TA.Extraocular mesenchyme patterns the optic vesicle during early eye development in the embryonic chick[J].Development,2000,127(21):4599-4609.
24 Araki M,Takano T,Uemonsa T,et al.Epithelia-mesenchyme interaction plays an essential role in trans differentiation of retinal pigment epithelium of silver mutant quail:Localization of FGFand related molecules and aberrant migration pattern of neural crest cells during eye rudiment formation[J].Developmental Biology,2002,244(2):358-371.
25 Talbot JC,Nichols JT,Yan YL,et al.Pharyngeal morphogenesis requires fras1-itga8-dependent epithelial-mesenchymal interaction[J].Developmental Biology,2016.416(1):136-148.
26 Cayuso J,Dzementsei A,Fischer JC,et al.EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality[J].Dev Cell,2016.39(3):316-328.
27 Lelli A,Kazmierczak O,Kawashima Y,et al.Development and Regeneration of Sensory Transduction in Auditory Hair Cells Requires Functional Interaction Between Cadherin-23 and Protocadherin-15[J].Journal of Neuroscience,2010,30(34):11259-11269.
28 Anniko M.Hair Cell-Differentiation Following Tissue Interactions for Induction of Otocyst Morphogenesis[J].Archives of Oto-Rhino-Laryngology-Archiv Fur Ohren-Nasen-Und Kehlkopfheilkunde,1985,242(3):287-294.
29 Trowe MO,Shah S,Petry M,et al.Loss of Sox9 in the periotic mesenchyme affects mesenchymal expansion and differentiation,and epithelial morphogenesis during cochlea development in the mouse[J].Dev Biol,2010.342(1):51-62.
30 Braunstein EM,Morrow BE,Adams JC,et al.Cooperative function of Tbx1 and Brn4 in the periotic mesenchyme is necessary for cochlea formation[J].J Assoc Res Otolaryngol,2008.9(1):33-43.
31 White PM,Doetzlhofer A,Yun SL,et al.Mammalian cochlear supporting cells can divide and trans-differentiate into hair cells[J].Nature,2006,441(7096):984-987.
32 Doetzlhofer A,White PM,Johnson JE,et al.In vitro growth and differentiation of mammalian sensory hair cell progenitors:a requirement for EGF and periotic mesenchyme[J].Developmental Biology,2004,272(2):42-447.
2 McCullar JS,Ty S,Campbell S,et al.Activin Potentiates Proliferation in Mature Avian Auditory Sensory Epithelium[J].Journal of Neuroscience,2010,30(2):478-490.
3 Stone JS,Cotanche DA.Hair cell regeneration in the avian auditory epithelium[J].International Journal of Developmental Biology,2007,51(6-7):633-647.
4 Corwin JT,Oberholtzer JC.Fish n'chicks:Model recipes for hair-cell regeneration?[J]Neuron,1997,19(5):951-954.
5 Lewis RM,Keller JJ,Wan L,et al.Bone morphogenetic protein4 antagonizes hair cell regeneration in the avian auditory epithelium[J].Hearing Research,2018.364:1-11.
6 Chardin S,Romand R.Regeneration and Mammalian Auditory Hair-Cells[J].Science,1995,267(5198):707-709.
7 Bermingham-McDonogh O,Reh TA.Regulated reprogramming in the regeneration of sensory receptor cells[J].Neuron,2011.71(3):389-405.
8 Hildebrand MS,Silva MGD,Klockars,et al.Gene expression profiling analysis of the inner ear[J].Hearing Research,2007,225(1-2):1-10.
9 Bermingham NA,Hassan BA,Price SD,et al.Math1:An essential gene for the generation of inner ear hair cells[J].Science,1999,284(5421):p.1837-1841.
10 Kiernan AE,Pelling AL,Leung KK,et al.Sox2 is required for sensory organ development in the mammalian inner ear[J].Nature,2005.434(7036):1031-1035.
11 Fumasoni I,Meani N,Rambaldi D,et al.Family expansion and gene rearrangements contributed to the functional specialization of PRDM genes in vertebrates[J].Bmc Evolutionary Biology,2007,7:187.
12 Zazzo ED,Rosa CD,Abbondanza C,et al.PRDM Proteins Molecular Mechanisms in Signal Transduction and Transcriptional Regulationdf[J].Biology,2013,2(1):107-141.
13 Nishikawa N,To Toyota M,Suzuki H,et al.Gene amplification and overexpression of PRDM14 in breast cancers[J].Cancer Research,2007,67(20):9649-9657.
14 Lang WJ,Chen FY.The reciprocal link between EVI1 and miR-NAs in human malignancies[J].Gene,2018.672:56-63.
15 Seale P,Bjork B,Yang W,et al.PRDM16 controls a brown fat/skeletal muscle switch[J].Nature,2008,454(7207):961-U27.
16 Moore AW,Jan LY,Jan YN.hamlet,a binary genetic switch between single-and multiple-dendrite neuron morphology[J].Science,2002,297(5585):1355-1358.
17 Alliston T,Ko TC,Cao Y,et al.Repression of Bone Morphogenetic Protein and Activin-inducible Transcription by Evi-1[J].Journal of Biological Chemistry,2005,280(25):24227-24237.
18 Dabdoub A,Puligilla C,Jones JM,et al.Sox2 signaling in prosensory domain specification and subsequent hair cell differentiation in the developing cochlea[J].Proceedings of the National Academy of Sciences of the United States of America,2008.105(47):18396-18401.
19 Hume CR,Bratt DL,Oesterle EC.Expression of LHX3 and SOX2 during mouse inner ear development[J].Gene Expression Patterns,2007.7(7):798-807.
20 Eguchi R,Yooshigai E,Koga T,et al.Spatiotemporal expression of Prdm genes during Xenopus development[J].Cytotechnology,2015,67(4):711-719.
21 Mead PE,Paraganas E,Ohtsuka S,et al.Evi-1 expression in Xenopus[J].Gene Expression Patterns,2005,5(5):601-608.
22刘少峰,李华伟,周梁.胚胎小鼠耳蜗感觉上皮的形态发生[J].中国耳鼻咽喉头颈外科.2005,12(7):433-436.Liu SF,Li HW,Zhou L.Morphogenesis in the sensory epithelia of embryonic mouse cochlea[J].Chinese Archives of Otolaryngology-head and Neck Surgery,2005,12(7):433-436).
23 Fuhrmann S,Levine EM,Reh TA.Extraocular mesenchyme patterns the optic vesicle during early eye development in the embryonic chick[J].Development,2000,127(21):4599-4609.
24 Araki M,Takano T,Uemonsa T,et al.Epithelia-mesenchyme interaction plays an essential role in trans differentiation of retinal pigment epithelium of silver mutant quail:Localization of FGFand related molecules and aberrant migration pattern of neural crest cells during eye rudiment formation[J].Developmental Biology,2002,244(2):358-371.
25 Talbot JC,Nichols JT,Yan YL,et al.Pharyngeal morphogenesis requires fras1-itga8-dependent epithelial-mesenchymal interaction[J].Developmental Biology,2016.416(1):136-148.
26 Cayuso J,Dzementsei A,Fischer JC,et al.EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality[J].Dev Cell,2016.39(3):316-328.
27 Lelli A,Kazmierczak O,Kawashima Y,et al.Development and Regeneration of Sensory Transduction in Auditory Hair Cells Requires Functional Interaction Between Cadherin-23 and Protocadherin-15[J].Journal of Neuroscience,2010,30(34):11259-11269.
28 Anniko M.Hair Cell-Differentiation Following Tissue Interactions for Induction of Otocyst Morphogenesis[J].Archives of Oto-Rhino-Laryngology-Archiv Fur Ohren-Nasen-Und Kehlkopfheilkunde,1985,242(3):287-294.
29 Trowe MO,Shah S,Petry M,et al.Loss of Sox9 in the periotic mesenchyme affects mesenchymal expansion and differentiation,and epithelial morphogenesis during cochlea development in the mouse[J].Dev Biol,2010.342(1):51-62.
30 Braunstein EM,Morrow BE,Adams JC,et al.Cooperative function of Tbx1 and Brn4 in the periotic mesenchyme is necessary for cochlea formation[J].J Assoc Res Otolaryngol,2008.9(1):33-43.
31 White PM,Doetzlhofer A,Yun SL,et al.Mammalian cochlear supporting cells can divide and trans-differentiate into hair cells[J].Nature,2006,441(7096):984-987.
32 Doetzlhofer A,White PM,Johnson JE,et al.In vitro growth and differentiation of mammalian sensory hair cell progenitors:a requirement for EGF and periotic mesenchyme[J].Developmental Biology,2004,272(2):42-447.