氧化硫硫杆菌JJU-1生物淋滤去除污泥中的重金属
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摘要
将前期分离得到的嗜酸性氧化硫硫杆菌JJU-1(Thiobacillus thiooxidans JJU-1)接种于污水处理厂污泥中,进行为期7 d的生物淋滤实验,对淋滤过程中的pH值、氧化还原电位(ORP)和重金属去除率进行检测。研究结果表明:氧化硫硫杆菌JJU-1不同接种量处理浸出液的pH下降幅度不同,当接种量为15%时,浸出液pH下降最快,其ORP达到最大值(444m V),Cu、Cd和Pb的去除率分别高达79.2%、96.6%和93.4%。添加能源物质S可以明显降低淋滤液的pH,当能源物质S投入量增加到12 g·L~(-1)时,淋滤7 d后,浸出液的pH下降至1以下,浸出液的ORP在S添加量为16 g·L~(-1)时达到最大值(477 m V),20 g·L~(-1)S粉添加量条件下,Cr、Cu和Pb的去除率最大,分别为98.8%、74.3%和85.8%;然而,添加能源物质S对Cd的浸出无明显影响。以上研究结果表明,氧化硫硫杆菌JJU-1在去除污泥中重金属方面有着广阔的前景。
In order to investigate the effect of Acidithiobacillus thiooxidans on bioleaching of Cd,Cr,Cu and Pbrich sludge,Acidithiobacillus thiooxidans JJU-1 was inoculated to sludge from a sewage disposal plant through flasks experiments for 7 days. The pH value,ORP(oxidation reduction potential) and the respective removal rates of bioleaching of the sludge were tested. The results showed that the descending range differed in different amounts of inoculums of JJU-1. The pH value decreased by the highest descending range and theORP reached the maximum of 444 m V with the sludge-inoculation ratio of 15%. As high as 79. 2% of Cu,96. 6% of Cd and93. 4% of Pb could be leached for 15% of sludge-inoculation ratio. In addition,the energy source of sulfur significantly reduced the pH value of sludge. The pH value declined to less than 1 with 12 g·L~(-1)of sulfur after 7days of bioleaching. TheORP exhibited the maximum of 477 m V with 16 g·L~(-1)of sulfur. The maximum removal rate of Cr,Cu and Pb were 98. 8%,74. 3% and 85. 8% with 20 g·L~(-1)of sulfur,respectively. However,the addition of sulfur did not affect the leaching rate of Cd. These results demonstrated the potential of Acidithiobacillus thiooxidans JJU-1 for the bioextraction of valuable metals from sludge.
In order to investigate the effect of Acidithiobacillus thiooxidans on bioleaching of Cd,Cr,Cu and Pbrich sludge,Acidithiobacillus thiooxidans JJU-1 was inoculated to sludge from a sewage disposal plant through flasks experiments for 7 days. The pH value,ORP(oxidation reduction potential) and the respective removal rates of bioleaching of the sludge were tested. The results showed that the descending range differed in different amounts of inoculums of JJU-1. The pH value decreased by the highest descending range and theORP reached the maximum of 444 m V with the sludge-inoculation ratio of 15%. As high as 79. 2% of Cu,96. 6% of Cd and93. 4% of Pb could be leached for 15% of sludge-inoculation ratio. In addition,the energy source of sulfur significantly reduced the pH value of sludge. The pH value declined to less than 1 with 12 g·L~(-1)of sulfur after 7days of bioleaching. TheORP exhibited the maximum of 477 m V with 16 g·L~(-1)of sulfur. The maximum removal rate of Cr,Cu and Pb were 98. 8%,74. 3% and 85. 8% with 20 g·L~(-1)of sulfur,respectively. However,the addition of sulfur did not affect the leaching rate of Cd. These results demonstrated the potential of Acidithiobacillus thiooxidans JJU-1 for the bioextraction of valuable metals from sludge.
引文
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[12]杨期勇,邱秀文,程鹏飞,等.嗜酸性氧化硫硫杆菌的分离鉴定及其产酸特性[J].生态环境学报,2015,24(8):1366-1374
[13]谭良良,张军,陈俊,等.一株氧化硫硫杆菌的分离鉴定及其沥滤污泥研究[J].中国给水排水,2014,30(7):21-26
[14]张再利,贾晓珊.两种硫杆菌对河涌底泥重金属的生物沥滤[J].中国环境科学,2008,28(7):624-629
[15]温烨明,王清萍,林鸿雁,等.污泥中氧化硫硫杆菌的分离及其应用效果[J].环境污染与防治,2009,31(7):52-55
[16]王聪,李强,李保宾,等.生物淋滤法去除污泥中的重金属[J].微生物学通报,2010,37(4):615-621
[17]TYAGI R D,TRAN F T.Bacterial leaching of metal from digested sewage sludge in continuous system[J].Environmental Technology Letter,1991,12:303-312
[18]周立祥,王艮梅.污水污泥中重金属的细菌淋滤效果研究[J].环境科学学报,2001,21(4):504-506
[2]李超,周立祥,王世梅.复合硫杆菌生物浸出污泥中重金属的效果及与p H和ORP的关系[J].环境科学学报,2008,28(6):1155-1160
[3]陈同斌,黄启飞,高定,等.中国城市污泥的重金属含量及其变化趋势[J].环境科学学报,2003,23(5):561-569
[4]HAGHSHENAS D F,BONAKDARPOUR B,ALAMDARI E K,et al.Optimization of physicochemical parameters for bioleaching of sphalerite by Acidithiobacillus ferrooxidans using shaking bioreactors[J].Hydrometallurgy,2012,111:22-28
[5]NOORI F Z,NEMATDOUST H B,AMOABEDINY G,et al.Anoptimized integrated process for the bioleaching of a spent refinery processing catalysts[J].International Journal of Environmental Research,2014,8(3):621-634
[6]PENG G,TIAN G.Using electrode electrolytes to enhance electrokinetic removal of heavy metals from electroplating sludge[J].Chemical Engineering Journal,2010,165(2):388-394
[7]毕文龙,崔雨琪,方迪,等.嗜酸硫杆菌和黑曲霉对电镀污泥重金属浸出效果[J].环境工程学报,2014,8(10):4402-4408
[8]霍敏波,郑冠宇,梁剑茹,等.生物沥浸处理中微生物菌群和胞外聚合物对城市污泥脱水性能的影响[J].环境科学学报,2014,34(9):2199-2204
[9]訾祥,侯庆杰,颜成,等.生物沥浸法对常规脱水污泥再深度脱水的效果[J].中国给水排水,2014,30(17):37-40
[10]YANG J,WANG Q,LUO Q,et al.Biosorption behavior of heavy metals in bioleaching process of MSWI fly ash by Aspergillus niger[J].Biochemical Engineering Journal,2009,46(3):294-299
[11]REN W X,LI P J,GENG Y,et al.Biological leaching of heavy metals from a contaminated soil by Aspergillusniger[J].Journal of Hazardous Materials,2009,167(1):164-169
[12]杨期勇,邱秀文,程鹏飞,等.嗜酸性氧化硫硫杆菌的分离鉴定及其产酸特性[J].生态环境学报,2015,24(8):1366-1374
[13]谭良良,张军,陈俊,等.一株氧化硫硫杆菌的分离鉴定及其沥滤污泥研究[J].中国给水排水,2014,30(7):21-26
[14]张再利,贾晓珊.两种硫杆菌对河涌底泥重金属的生物沥滤[J].中国环境科学,2008,28(7):624-629
[15]温烨明,王清萍,林鸿雁,等.污泥中氧化硫硫杆菌的分离及其应用效果[J].环境污染与防治,2009,31(7):52-55
[16]王聪,李强,李保宾,等.生物淋滤法去除污泥中的重金属[J].微生物学通报,2010,37(4):615-621
[17]TYAGI R D,TRAN F T.Bacterial leaching of metal from digested sewage sludge in continuous system[J].Environmental Technology Letter,1991,12:303-312
[18]周立祥,王艮梅.污水污泥中重金属的细菌淋滤效果研究[J].环境科学学报,2001,21(4):504-506