乳酸菌对氨基甲酸酯农药的降解作用
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摘要
氨基甲酸酯农药是人类针对有机氯和有机磷农药的缺点而开发的一种新型广谱杀虫、杀螨、除草剂。本研究利用保加利亚乳杆菌、嗜热链球菌和植物乳杆菌单独及其混合作用于异丙威、仲丁威和呋喃丹,结果发现除植物乳杆菌和嗜热链球菌混合对异丙威的降解效果低于仅添加嗜热链球菌外,其它混合培养菌株的降解效果均优于只添加一种菌株。在MRS中培养24 h,保加利亚乳杆菌和嗜热链球菌对仲丁威和呋喃丹的降解率分别达37.38%和38.27%,保加利亚乳杆菌和植物乳杆菌对异丙威的降解率达39.15%。
The carbamate pesticide is a kind of new broad insecticide, ixodicide, herbicide, which is developed by the human being to avoid the shortcomings of organic chlorine and the organic phosphorus pesticide. In this paper, the Lactobacillus bulgaricus, Streptococcus thermophilus and L. plantarum were used alone and its combination to biodegradate isoprocarb, fenobucarb and carbofuran. The result was that in addition to the degradation effect of isoprocarb by Lactobacillus bulgaricus and Streptococcus thermophilus was lower than that of the addition of Streptococcus thermophilus,the degradation effect of other mixed culture strains was better than that of only one strain. In MRS broth for 24 h, the degradation effect of fenobucarb and carbofuran could reach 37.38% and 38.27% by Lactobacillus bulgaricus and Streptococcus thermophilus, and the degradation of isoprocarb could reach 39.15%.
The carbamate pesticide is a kind of new broad insecticide, ixodicide, herbicide, which is developed by the human being to avoid the shortcomings of organic chlorine and the organic phosphorus pesticide. In this paper, the Lactobacillus bulgaricus, Streptococcus thermophilus and L. plantarum were used alone and its combination to biodegradate isoprocarb, fenobucarb and carbofuran. The result was that in addition to the degradation effect of isoprocarb by Lactobacillus bulgaricus and Streptococcus thermophilus was lower than that of the addition of Streptococcus thermophilus,the degradation effect of other mixed culture strains was better than that of only one strain. In MRS broth for 24 h, the degradation effect of fenobucarb and carbofuran could reach 37.38% and 38.27% by Lactobacillus bulgaricus and Streptococcus thermophilus, and the degradation of isoprocarb could reach 39.15%.
引文
[1] GEBREMICHAEL S, BIRHANU T, TESSEMA D A. Analysis of organochlorine pesticide residues in human and cow's milk in the towns of Asendabo, Serbo and Jimma in South-Western Ethiopia[J]. Chemosphere, 2013, 90(5):1652-1659.
[2] WANG X, JING C, ZHOU H, et al. Development of a simple combining apparatus to perform a magnetic stirringassisted dispersive liquid-liquid microextraction and its application for the analysis of carbamate and organophosphorus pesticides in tea drinks[J]. Analytica Chimica Acta, 2013, 787(13):71-77.
[3] MA X, WANG J, WU Q, et al. Extraction of carbamate pesticides in fruit samples by graphene reinforced hollow fibre liquid microextraction followed by high performance liquid chromatographic detection[J]. Food Chemistry, 2014,157(11):119-124.
[4] PRASAD R, UPADHYAY N, KUMAR V. Simultaneous determination of seven carbamate pesticide residues in gram, wheat, lentil, soybean, fenugreek leaves and apple matrices[J]. Microchemical Journal, 2013, 111(111):91-96.
[5] SAGRATINI G, MA譙ES J, GIARDIN魣D, et al. Analysis of carbamate and phenylurea pesticide residues in fruit juices by solid-phase microextraction and liquid chromatography-mass spectrometry[J]. Journal of Chromatography A,2007, 1147(2):135-143.
[6] LOZOWICKA B, KACZYNSKI P, PARITOVAАЕ, et al. Pesticide residues in grain from Kazakhstan and potential health risks associated with exposure to detected pesticides[J]. Food&Chemical Toxicology an International Journal Published for the British Industrial Biological Research Association, 2013, 64(2):238-248.
[7] GUO Q, XIA L V, LI T. Simultaneous determination of 26 pesticide residues in 5 Chinese medicinal materials using solid-phase extraction and GC-ECD method[J]. Chinese Journal of Natural Medicines, 2009, 7(3):210-216.
[8] CONG H, MAN H, CHEN B, et al. A sol-gel polydimethylsiloxane/polythiophene coated stir bar sorptive extraction combined with gas chromatography-flame photometric detection for the determination of organophosphorus pesticides in environmental water samples[J]. Journal of Chromatography A, 2013, 1275(2):25-31.
[9] N魷BREGA I C C, PEREIRA G E, SILVA M, et al. Improved sample preparation for GC-MS-SIM analysis of ethyl carbamate in wine[J]. Food Chemistry, 2015, 177(2):23-28.
[10] MO W M, HE H L, XU X M, et al. Simultaneous determination of ethyl carbamate, chloropropanols and acrylamide in fermented products, flavoring and related foods by gas chromatography-triple quadrupole mass spectrometry[J]. Food Control, 2014, 43(8):251-257.
[11] KOLVENBACH B A, HELBLING D E, KOHLER H P E, et al. Emerging chemicals and the evolution of biodegradation capacities and pathways in bacteria[J]. Current Opinion in Biotechnology, 2014, 27(27):8-14.
[12] YONG Y C, ZHONG J J. Recent advances in biodegradation in China:New microorganisms and pathways,biodegradation engineering, and bioenergy from pollutant biodegradation[J]. Process Biochemistry, 2010, 45(12):1937-1943.
[13] JIANG J, ZHANG R, LI R, et al. Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon[J]. Biodegradation, 2007, 18(4):403-412.
[14]苗丽,马艳艳,张子宏,等.进出口食品中菌落总数计数方法:SN/T 0168-2015[S].北京:中国标准出版社,2016:3-7.
[2] WANG X, JING C, ZHOU H, et al. Development of a simple combining apparatus to perform a magnetic stirringassisted dispersive liquid-liquid microextraction and its application for the analysis of carbamate and organophosphorus pesticides in tea drinks[J]. Analytica Chimica Acta, 2013, 787(13):71-77.
[3] MA X, WANG J, WU Q, et al. Extraction of carbamate pesticides in fruit samples by graphene reinforced hollow fibre liquid microextraction followed by high performance liquid chromatographic detection[J]. Food Chemistry, 2014,157(11):119-124.
[4] PRASAD R, UPADHYAY N, KUMAR V. Simultaneous determination of seven carbamate pesticide residues in gram, wheat, lentil, soybean, fenugreek leaves and apple matrices[J]. Microchemical Journal, 2013, 111(111):91-96.
[5] SAGRATINI G, MA譙ES J, GIARDIN魣D, et al. Analysis of carbamate and phenylurea pesticide residues in fruit juices by solid-phase microextraction and liquid chromatography-mass spectrometry[J]. Journal of Chromatography A,2007, 1147(2):135-143.
[6] LOZOWICKA B, KACZYNSKI P, PARITOVAАЕ, et al. Pesticide residues in grain from Kazakhstan and potential health risks associated with exposure to detected pesticides[J]. Food&Chemical Toxicology an International Journal Published for the British Industrial Biological Research Association, 2013, 64(2):238-248.
[7] GUO Q, XIA L V, LI T. Simultaneous determination of 26 pesticide residues in 5 Chinese medicinal materials using solid-phase extraction and GC-ECD method[J]. Chinese Journal of Natural Medicines, 2009, 7(3):210-216.
[8] CONG H, MAN H, CHEN B, et al. A sol-gel polydimethylsiloxane/polythiophene coated stir bar sorptive extraction combined with gas chromatography-flame photometric detection for the determination of organophosphorus pesticides in environmental water samples[J]. Journal of Chromatography A, 2013, 1275(2):25-31.
[9] N魷BREGA I C C, PEREIRA G E, SILVA M, et al. Improved sample preparation for GC-MS-SIM analysis of ethyl carbamate in wine[J]. Food Chemistry, 2015, 177(2):23-28.
[10] MO W M, HE H L, XU X M, et al. Simultaneous determination of ethyl carbamate, chloropropanols and acrylamide in fermented products, flavoring and related foods by gas chromatography-triple quadrupole mass spectrometry[J]. Food Control, 2014, 43(8):251-257.
[11] KOLVENBACH B A, HELBLING D E, KOHLER H P E, et al. Emerging chemicals and the evolution of biodegradation capacities and pathways in bacteria[J]. Current Opinion in Biotechnology, 2014, 27(27):8-14.
[12] YONG Y C, ZHONG J J. Recent advances in biodegradation in China:New microorganisms and pathways,biodegradation engineering, and bioenergy from pollutant biodegradation[J]. Process Biochemistry, 2010, 45(12):1937-1943.
[13] JIANG J, ZHANG R, LI R, et al. Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon[J]. Biodegradation, 2007, 18(4):403-412.
[14]苗丽,马艳艳,张子宏,等.进出口食品中菌落总数计数方法:SN/T 0168-2015[S].北京:中国标准出版社,2016:3-7.