四川华鳊精子发生显微及超微结构观察
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摘要
对四川华鳊(Sinibrama taeniatus)精子发生过程进行了显微及超微结构观察。结果显示:在四川华鳊精子发生过程中,生精细胞嗜碱性逐渐增强,细胞体积渐小;精原细胞可分为两种,二者在显微及超微结构上均有明显区别;从精原细胞分裂开始就出现胞质不完全分裂,相邻细胞通过细胞质桥连接,并在精子细胞变态中后期消失;初级精母细胞核仁在减数第一次分裂开始后会逐渐消失;精子细胞变态过程中可观察到互相垂直的基体和近端中心粒,晚期出现中心粒附属物并在精子成熟前逐渐萎缩至消失,近端中心粒也在精子成熟时消失;四川华鳊精子头部近圆形或稍不规则,无顶体,核空泡小,核凹窝不发达,尾部具两侧对称的侧鳍,轴丝为"9+2"双联体微管结构。
The spermatogenesis of Sinibrama taeniatus was investigated by histological and ultrastructural methods in the present study. The results showed that the volume of germ cells gradually decreased with the strengthen of cell basophilia in the process of spermatogenesis. Spermatogonia cells could be distinguished as two types by their microscopic and ultrastructural characteristic. The incomplete division of cytoplasm dated from the spermatogonia cells and the intercellular cytoplasm bridge occurred to contact adjacent cells which would disappear in the late stage of spermiogenesis. The nucleolus of primary spermatocytes would eventually disappear after the first meiosis. The basal body and proximal centriole were observed to be perpendicular to each other during spermiogenesis, and the centriolar appendage arose in the late stage and disappeared with proximal centriole before sperm maturation. The head of S. taeniatus sperms were nearly round or slightly irregular with small nuclear vesicles, undeveloped nuclear pocket, symmetrical lateral fin and no acrosome. In addition, the axoneme of sperm was "9+2" duplex microtubule.
The spermatogenesis of Sinibrama taeniatus was investigated by histological and ultrastructural methods in the present study. The results showed that the volume of germ cells gradually decreased with the strengthen of cell basophilia in the process of spermatogenesis. Spermatogonia cells could be distinguished as two types by their microscopic and ultrastructural characteristic. The incomplete division of cytoplasm dated from the spermatogonia cells and the intercellular cytoplasm bridge occurred to contact adjacent cells which would disappear in the late stage of spermiogenesis. The nucleolus of primary spermatocytes would eventually disappear after the first meiosis. The basal body and proximal centriole were observed to be perpendicular to each other during spermiogenesis, and the centriolar appendage arose in the late stage and disappeared with proximal centriole before sperm maturation. The head of S. taeniatus sperms were nearly round or slightly irregular with small nuclear vesicles, undeveloped nuclear pocket, symmetrical lateral fin and no acrosome. In addition, the axoneme of sperm was "9+2" duplex microtubule.
引文
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[20]林光华,林琼,胡成钰,等.草鱼、兴国红鲤和革胡子鲇精子超微结构的比较研究[J].南昌大学学报,1998,22(3):283-287.
[21]王冰,万全,李飞,等.刀鲚精子超微结构研究[J].水生态学杂志,2010,3(3):57-63.
[22]胡雨,高胜涛,刘亚秋,等.泥鳅精子和卵子结构及受精过程的细胞学观察[J].水生生物学报,2016,40(5):942-950.
[23]张耀光,罗泉笙,钟明超.长吻鮠精巢发育的分期及精子发生和形成[J].动物学研究,1992,13(3):281-287.
[24]李飞.胭脂鱼精子结构研究[J].安徽农业大学学报,2009,36(2):260-266.
[25]李飞,万全,黄鲜明.大鳞副泥鳅精子结构研究[J].生物学杂志,2011,28(4):26-30.
[26]王永明,陈瑜,胡雨,等.宽体沙鳅精子超微结构及Na+、K+、Ca2+对其精子活力的影响[J].四川动物,2016,35(1):38-43.
[27]张汉峰,魏刚,黄林,等.短尾鮠精子细胞和精子的超微结构研究[J].西南农业大学学报,2005,27(5):696-699.
[28]陈永强,魏刚,李振华,等.粗唇鮠精巢显微结构和精子发生的研究[J].淡水渔业,2008,38(1):6-10.
[29]张涛,邹桂伟,梁宏伟,等.四种罗非鱼精子超微结构的比较[J].淡水渔业,2015,45(5):29-34.
[30]Deurs B V,Lastein U.Ultrastructure of spermatozoa of the teleost Pantodon buchholzi peters,with particular reference to the midpiece [J].Fish Physiol,1973,42(5):517-533.
[31]Billard R.Spermatogenesis and spermatology of some teleost fish species [J].Reprod Nutr Dev,1986,26(4):877-920.
[32]Afzelius B A.Fine structure of the garfish spermatozoon [J].J Ultra Mol Struct R,1978,64(3):309-314.
[33]刘文彬,张轩杰.黄颡鱼精巢发育和周年变化及精子的发生与形成[J].湖南师范大学自然科学学报,2004,27(1):66-70.
[34]李胜忠,程先友,董亚菲,等.江鳕精子的形态及超微结构观察[J].贵州农业科学,2016,44(5):93-96.
[35]赵峰,庄平,章龙珍,等.银鲳精子的超微结构[J].海洋渔业,2010,32(4):383-387.
[36]Poirier G R,Nicholson N.Fine structure of the testicular spermatozoa from the channel catfish,lctalurus punctatus [J].J Ultra Mol Struct R,1982,80(1):104-110.
[37]Stoss J.6 Fish gamete preservation and spermatozoon physiology [J].Fish Physiol,1983,(9):307-308.
[38]尹洪滨,孙忠武.索氏六须鲶精子的超微结构[J].水产学报,2000,24(4):302-305.
[2]解崇友,倪露芸,吴迪,等.岷江眉山段四川华鳊年龄与生长[J].四川动物,2016,35(1):78-83.
[3]熊飞,刘红艳,段辛斌,等.长江上游江津段鱼类群落结构及资源利用[J].安徽大学学报,2014,38(3):94-102.
[4]李斌,解崇友,张婷,等.岷江眉山段四川华鳊的繁殖生物学资料[J].动物学杂志,2015,50(4):563-570.
[5]芮菊生.组织切片技术[M].北京:高等教育出版社,1978:98-106.
[6]Sommerville J.Cell biology:Organizing the nucleolus [J].Nature,1985,318(6045):410-411.
[7]康现江,梁春光,郭明申,等.半滑舌鳎精子发生和精子形成的超微结构[J].动物学报,2008,54(2):356-365.
[8]车静.大眼鳜精巢及精子发育[D].重庆:西南大学,2003.
[9]管汀鹭,黄丹青,黄国屏.金鱼精巢的细胞构造与精子的发生和形成[J].水生生物学报,1990,14(3):233-238.
[10]洪万树,翁幼竹,林君卓,等.鲻鱼精子发生和形成的超微结构研究[J].海洋学报,2001,23(5):116-122.
[11]郭明申,刘龙,穆淑梅,等.孔雀鱼精子发生的显微与超微结构[J].河北大学学报,2006,26(6):653-658.
[12]Tian X F,Yuan L J,Li Y H,et al Ultrastructure observation on the transformation of the spermatozoon in spermatogenesis of Taeniid cestodes [J].Chinese J Parasitol Parasitic Dis,1998,16(4):269-273.
[13]Quagio G I,Lello E D.Cytoplasmic bridges,intercellular junction,and individualization of germ cells during spermatogenesis in Dermatobia hominis(Diptera:Cuterebridae) [J].Mol Biol Cell,2003,14(7):2768-2780.
[14]Ventela S,Toppari J,Parvinen M.Intercellular organelle traffic through cytoplasmic bridges in early spermatid of the Rat:mechanism of haploid gene product sharing [J].Mol Biol Cell,1996,227(2):145-154.
[15]管汀鹭.金鱼精子发生中的拟染色质小体[J].动物学报,1989,35(2):124-129.
[16]Walt H,Armbruster B L.Actin and RNA are compenents of the chromatoid bodies in spermatids of the rat [J].Cell Tissue Res,1984,236(2):487-490.
[17]林丹军,尤永隆.褐菖鲉精细胞晚期的变化及精子结构研究[J].动物学研究,1998,19(5):359-366.
[18]Fawcett D W,Phillips D M.The fine structure and development of the neck of the mammalian spermatozoon [J].Anat Rec,1969,165(2):153-164.
[19]王志坚,殷江霞,张耀光.长薄鳅的精巢发育和精子发生[J].淡水渔业,2009,39(1):3-9.
[20]林光华,林琼,胡成钰,等.草鱼、兴国红鲤和革胡子鲇精子超微结构的比较研究[J].南昌大学学报,1998,22(3):283-287.
[21]王冰,万全,李飞,等.刀鲚精子超微结构研究[J].水生态学杂志,2010,3(3):57-63.
[22]胡雨,高胜涛,刘亚秋,等.泥鳅精子和卵子结构及受精过程的细胞学观察[J].水生生物学报,2016,40(5):942-950.
[23]张耀光,罗泉笙,钟明超.长吻鮠精巢发育的分期及精子发生和形成[J].动物学研究,1992,13(3):281-287.
[24]李飞.胭脂鱼精子结构研究[J].安徽农业大学学报,2009,36(2):260-266.
[25]李飞,万全,黄鲜明.大鳞副泥鳅精子结构研究[J].生物学杂志,2011,28(4):26-30.
[26]王永明,陈瑜,胡雨,等.宽体沙鳅精子超微结构及Na+、K+、Ca2+对其精子活力的影响[J].四川动物,2016,35(1):38-43.
[27]张汉峰,魏刚,黄林,等.短尾鮠精子细胞和精子的超微结构研究[J].西南农业大学学报,2005,27(5):696-699.
[28]陈永强,魏刚,李振华,等.粗唇鮠精巢显微结构和精子发生的研究[J].淡水渔业,2008,38(1):6-10.
[29]张涛,邹桂伟,梁宏伟,等.四种罗非鱼精子超微结构的比较[J].淡水渔业,2015,45(5):29-34.
[30]Deurs B V,Lastein U.Ultrastructure of spermatozoa of the teleost Pantodon buchholzi peters,with particular reference to the midpiece [J].Fish Physiol,1973,42(5):517-533.
[31]Billard R.Spermatogenesis and spermatology of some teleost fish species [J].Reprod Nutr Dev,1986,26(4):877-920.
[32]Afzelius B A.Fine structure of the garfish spermatozoon [J].J Ultra Mol Struct R,1978,64(3):309-314.
[33]刘文彬,张轩杰.黄颡鱼精巢发育和周年变化及精子的发生与形成[J].湖南师范大学自然科学学报,2004,27(1):66-70.
[34]李胜忠,程先友,董亚菲,等.江鳕精子的形态及超微结构观察[J].贵州农业科学,2016,44(5):93-96.
[35]赵峰,庄平,章龙珍,等.银鲳精子的超微结构[J].海洋渔业,2010,32(4):383-387.
[36]Poirier G R,Nicholson N.Fine structure of the testicular spermatozoa from the channel catfish,lctalurus punctatus [J].J Ultra Mol Struct R,1982,80(1):104-110.
[37]Stoss J.6 Fish gamete preservation and spermatozoon physiology [J].Fish Physiol,1983,(9):307-308.
[38]尹洪滨,孙忠武.索氏六须鲶精子的超微结构[J].水产学报,2000,24(4):302-305.