肌醇对小麦萌发期耐盐性的调节作用及生理机制分析
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摘要
以小麦品种西农979为材料,确定了用于小麦盐胁迫的NaCl浓度为300 mmol·L~(-1),然后通过添加1、2、4、8 mmol·L~(-1)的肌醇,观测在肌醇作用下小麦种子发芽率及丙二醛(MDA)含量。结果发现8 mmol·L~(-1)外源肌醇可使受盐胁迫的小麦种子7 d发芽率提高21. 3%,MDA含量下降41. 8%,MDA含量接近未受胁迫的小麦水平。同时,检测了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性,添加肌醇使盐胁迫下小麦SOD和CAT酶活性分别提高22. 9%、15. 0%,POD酶活性降低19. 7%。研究表明,肌醇显著降低盐胁迫下小麦发芽期的丙二醛含量,对盐胁迫下小麦的损伤起到了一定的缓解作用。
Using Xinong 979 of wheat variety as the material,NaCl concentration for wheat salt stress was confirmed to be 300 mmol·L~(-1). The inositol of 1,2,4 and 8 mmol·L~(-1) were added to test the germination rate of wheat seeds and malondialdehyde( MDA) content under the action of inositol. The results showed that the exogenous inositol of 8 mmol · L~(-1) could increase the germination rate by21. 3% and decrease the MDA content by 41. 8% on 7 days of wheat seeds germination under salt stress. The MDA content was close to that of unstressed wheat. Meanwhile,the activities of superoxide dismutase( SOD),catalase( CAT),peroxidase( POD) were also detected. The results showed that the SOD and CAT enzyme activities increased 22. 9% and 15. 0%,the POD enzyme activity decreased19. 7% by adding to the inositol for the wheat under the NaCl stress. The study indicated that the extra inositol could reduce the MDA content obviously and alleviate the damage of wheat under salt stress.
Using Xinong 979 of wheat variety as the material,NaCl concentration for wheat salt stress was confirmed to be 300 mmol·L~(-1). The inositol of 1,2,4 and 8 mmol·L~(-1) were added to test the germination rate of wheat seeds and malondialdehyde( MDA) content under the action of inositol. The results showed that the exogenous inositol of 8 mmol · L~(-1) could increase the germination rate by21. 3% and decrease the MDA content by 41. 8% on 7 days of wheat seeds germination under salt stress. The MDA content was close to that of unstressed wheat. Meanwhile,the activities of superoxide dismutase( SOD),catalase( CAT),peroxidase( POD) were also detected. The results showed that the SOD and CAT enzyme activities increased 22. 9% and 15. 0%,the POD enzyme activity decreased19. 7% by adding to the inositol for the wheat under the NaCl stress. The study indicated that the extra inositol could reduce the MDA content obviously and alleviate the damage of wheat under salt stress.
引文
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[2]魏博娴.中国盐碱土的分布与成因分析[J].水土保持应用技术,2012(6):27-28.
[3]吕金岭.盐碱对小麦生育的影响[J].盐碱地利用,1990(4):36-38.
[4]王克荣.次生盐碱地小麦高产栽培技术[J].现代农业,2014(11):37-41.
[5]李合生.现代植物生理学[M].北京:高等教育出版社,2002.
[6]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
[7]王玲丽,贾文杰,马璐琳,等.低温胁迫对不同百合主要生理指标的影响[J].植物生理学报,2014,50(9):1413-1422.
[8]马原松,辛倩,朱晓琴,等.精胺对盐胁迫下小麦幼苗生理生化指标的影响[J].江苏农业科学,2018,46(3):53-56.
[9] EASTCOTT E V. Wildiers’Bios[J]. The Journal of Physical Chemistry,1928,32(7):1094-1111.
[10] SCHELL J,KONCZ C,SPENA A,et al. Genes involved in the control of growth and differentiation in plants[J].Gene,1993,135(1/2):245-249.
[11] LOEWUS F A,KELLY S,NEUFELD E F. Metabolism of myo-inositol in plants:conversion to pectin,hemicellulose,d-xylose,and sugar acids[J]. Proc Natl Acad Sci USA,1962,48(3):421-425.
[12] DELHON C,THIéBAULT S. The migration of beech(Fagus sylvatica L.)up the Rhone:the Mediterranean history of a“mountain”species[J]. Vegetation History and Archaeobotany,2005,14(2):119-132.
[13] HORBOWICZ M,OBENDORF R L. Seed desiccation tolerance and storability:dependence on flatulence-producing oligosaccharides and cyclitols-review and survey[J]. Seed Science Research,2008,4(4):385-405.
[14] BISWAS B B,BISWAS S. myo-Inositol phosphates,phosphoinositides,and signal transduction[M]. Boston,MA:Springer,1996.
[15] LOEWUS F A,MURTHY P P N. myo-inositol metabolism in plants[J]. Plant Science,2000,150(1):1–19.
[16] NELSON D E,KOUKOUMANOS M,BOHNERT H J.Myo-inositol-dependent sodium uptake in ice plant[J].Plant Physiol,1999,119(1):165-172.
[17]张志良,瞿伟菁.植物生理学实验指导[M].第3版.北京:高等教育出版社,2003.
[18]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[19] DUNCAN D B. Multiple range and multiple F tests[J].Biometrics,1955,11(1):1-42.
[20]李金亭,赵萍萍,邱宗波,等.外源H2O2对盐胁迫下小麦幼苗生理指标的影响[J].西北植物学报,2012,32(9):1796-1801.