亚致死剂量氯吡嘧磺隆胁迫对斑马鱼典型肝脏代谢物的影响
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摘要
为获知水环境中亚致死剂量的氯吡嘧磺隆对斑马鱼肝脏的影响,将斑马鱼暴露在亚致死剂量的氯吡嘧磺隆药液中8 d,采用超高效液相色谱与四级杆-轨道离子阱质谱串联对斑马鱼的肝脏代谢物进行分析,采用SIEVE软件将图谱信息转换为含有保留时间、质荷比和相对峰面积的二维数据阵.同时,结合mz Cloud、Human Metabolome Database(HMDB)数据库共鉴定出33种代谢物,包括氨基酸、糖、有机酸等物质.最后,采用SIMCA软件对正离子和负离子模式下的检测结果进行主成分、偏最小二乘法和正交校正的偏最小二乘法分析.主成分和偏最小二乘法结果表明,对照组与处理组在2种检测模式下均存在差异代谢物.为了寻找造成对照组与处理组之间差异的代谢物,对代谢物进行S-Plot分析,结果表明,肌酸酐、牛磺酸、多巴胺和5b-胆甾醇硫酸酯组间差异最大(p<0.01).4种代谢产物可作为斑马鱼在氯吡嘧磺隆亚致死剂量胁迫下肝脏代谢异常的潜在标记物.
We applied a sub-lethal dose of halosulfuron-methyl stress to zebrafish in order to study the changes in metabolites in the liver. Ultra-high performance liquid chromatography coupled with Q Exactive hybrid quadrupole-orbitrap mass spectrometry( UPLC-MS/MS) was applied to analyze the effects of the sub-lethal dose of halosulfuron-methyl on metabolites in the liver of zebrafish.Each chromatogram obtained from UPLC-MS/MS analysis was pre-processed using SIEVE software to generate discrete peak lists containing m/z,retention time,and relative peak area.Using the mz Cloud and Human Metabolome Database,33 metabolites were identified including amino acids,sugars,and organic acids. The pre-processed UPLC-MS/MS data were exported to SIMICA-P 13.0 for analysis by using multivariate statistical methods. Three multivariate analysis methods were used to determine the optimal separation of clusters: principal component analysis,partial least squares-discriminant analysis,and orthogonal projection to latent structure-discriminant analysis.The results revealed significant differences( p < 0. 01) between the control and treatment groups. A representative S-plot showed the variables responsible for the separation.Creatinine,taurine,dopamine,and 5b-cyprinol sulfate appeared to be the most affecting variables( p < 0. 01). These four metabolites were identified as potential indicators of halosulfuron-methyl-induced liver abnormalities.
We applied a sub-lethal dose of halosulfuron-methyl stress to zebrafish in order to study the changes in metabolites in the liver. Ultra-high performance liquid chromatography coupled with Q Exactive hybrid quadrupole-orbitrap mass spectrometry( UPLC-MS/MS) was applied to analyze the effects of the sub-lethal dose of halosulfuron-methyl on metabolites in the liver of zebrafish.Each chromatogram obtained from UPLC-MS/MS analysis was pre-processed using SIEVE software to generate discrete peak lists containing m/z,retention time,and relative peak area.Using the mz Cloud and Human Metabolome Database,33 metabolites were identified including amino acids,sugars,and organic acids. The pre-processed UPLC-MS/MS data were exported to SIMICA-P 13.0 for analysis by using multivariate statistical methods. Three multivariate analysis methods were used to determine the optimal separation of clusters: principal component analysis,partial least squares-discriminant analysis,and orthogonal projection to latent structure-discriminant analysis.The results revealed significant differences( p < 0. 01) between the control and treatment groups. A representative S-plot showed the variables responsible for the separation.Creatinine,taurine,dopamine,and 5b-cyprinol sulfate appeared to be the most affecting variables( p < 0. 01). These four metabolites were identified as potential indicators of halosulfuron-methyl-induced liver abnormalities.
引文
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Wu S F,Xu G,Qi Y X,et al.2013.Advances in insect dopamine and it receptors[J].Acta Entomologica Sinica,56(11):1342-1358
Xu H D,Wang J S,Li M H,et al.2015.1HNMR based metabolomic approach to study the toxic effects of herbicide butachlor on goldfis(Carassius auratus)[J].Aquatic Toxicology,159:69-80
Zhang X T,Breksa A P,Mishchuk D O,et al.2012.Fertilisation an pesticides affect mandarin orange nutrient composition[J].Foo Chemistry 134,1020-1024
张铎,侯志广,邬美男,等.2014.氯吡嘧磺隆的水解动力学研究[J]中国农学通报,30(35):310-313
张铎.2014.氯吡嘧磺隆的水解与光解特性研究[D].长春:吉林农业大学
钟红舰,刘进玺,吴绪金,等.2011.75%氯吡嘧磺隆水分散粒剂在玉米及土壤中的消解动态与残留[J].农药,50(12):906-908
Beni C,Marconi S,Boccia P,et al.2011.Use of arsenic contaminated irrigation water for lettuce cropping:effects on soil,groundwater,and vegetal[J].Biological Trace Element Research,143(1):518-529
Bernillon S,Biais B,Deborde C,et al.2013.Metabolomic and elemental profiling of melon fruit quality as affected by genotype and environment[J].Metabolomics,9(1):57-77
Cocchetto D M,Tschanz C,Bjornsson T D.1983.Decreased rate of creatinine production in patients with hepatic disease:implications for estimation of creatinine clearance[J].Therapeutic Drug Monitoring,5(2):161-168
De Luca A,Pierno S,Camerino D C.2015.Taurine:the appeal of a safe amino acid for skeletal muscle disorders[J].Journal of Translational Medicine,13(1):243
郭敏,单正军,石利利,等.2012.三种磺酰脲类除草剂在土壤中的降解及吸附特性[J].环境科学学报,32(6):1459-1464
Hédiji H,Djebali W,Cabasson C,et al.2010.Effects of long-term cadmium exposure on growth and metabolomic profile of tomato plant[J].Ecotoxicology and Environmental Safety,73(8):1965-1974
黄上峰,王辉,黄燕敏,等.2008.胆甾醇硫酸酯钠及其类似物的合成研究[J].广西科学,15(4):414-418
Karmakar R,Bhattacharya R,Kulshrestha G.2009.Comparativ metabolite profiling of the insecticide thiamethoxam in plant and cel suspension culture of tomato[J].Journal of Agricultural and Foo Chemistry,57(14):6369-6374
Kausch K D,Sobolev A P,Goyal R K,et al.2012.Methyl jasmonat deficiency alters cellular metabolome,including the aminome o tomato(Solanum lycopersicum L.)fruit[J].Amino Acids,42(2/3)843-856
Kim J,Jung Y,Song B,et al.2013.Discrimination of cabbage(Brassic rapa ssp pekinensis)cultivars grown in different geographical area using1H NMR-based metabolomics[J].Food Chemistry,137(1)68-75
Lam N P,Sperelakis R,Kuk J,et al.1999.Rapid estimation of creatinin clearances in patients with liver dysfunction[J].Digestive Disease and Sciences,44(6):1222-1227
Li M H,Wang J S,Lu Z G,et al.2014.NMR-based metabolomic approach to study the toxicity of lambda-cyhalothrin to goldfis(Carassius auratus)[J].Aquatic Toxicology,146:82-92
Liu Y,Chen T,Li M H,et al.2015.1H NMR based metabolomic approach to study the toxic effects of dichlorvos on goldfish(Carassiu auratus)[J].Chemosphere,138:537-545
Milakofsky L,Hare T A,Miller J M,et al.1985.Rat plasma levels of amin acids and related compounds during stress[J].Life Sciences,36(8)753-761
Milakofsky L,Harris N,Vogel W H.1993.Effect of repeated stress o plasma catecholamines and taurine in young and old rats[J]Neurobiol Aging,14(4):359-366
Mustard J A,Beggs K T,Mercer A R.2005.Motercular biology of th invertebrate dopamine receptors[J].Archives of Insect Biochemistr and Physiology,59(3):103-117
Papadakis M A,Arieff A I.1987.Unpredictability of clinical evaluation o renal function in cirrhosis:prospective study[J].The America Journal of Medicine,82(5):945-952
Pereira S I,Figueiredo P I,Barros A S,et al.2014.Changes in th metabolome of lettuce leaves due to exposure to mancozeb pesticid[J].Food Chemistry,154:291-298
Picone G,Mezzetti B,Babini E,et al.2011.Unsupervised principa component analysis of NMR metabolic profiles for the assessment o substantial equivalence of transgenic grapes(Vitis vinifera)[J]Journal of Agricultural and Food Chemistry,59(17):9271-9279
Russel M H,Carskim T H,Mckelvey R.1995.A Risk evaluation of th leaching of sulfonylurea herbicides[J].Weeds,2:701-706
Schaffer S W,Jong C J,Ramila K C,et al.2010.Physiologicalroleso taurine in heart and muscle[J].Journal of Biomedical Science,1(1):1-8
Sobolev A P,Capitani D,Giannino D,et al.2010.NMR-metaboli methodology in the study of GM foods[J].Nutrients,2:1-15
Son H S,Hwang G S,Kim K M,et al.2009.Metabolomic studies on geographical grapes and their wines using1H NMR analysis coupled with multivariate statistics[J].Journal of Agricultural and Food Chemistry,57:1481-1490
Spraul M,Schütz B,Humpfer E,et al.2009.Mixture analysis by NMR a applied to fruit juice quality control[J].Magnetic Resonance in Chemistry,47(S1):S130-S137
Wu S F,Xu G,Qi Y X,et al.2013.Advances in insect dopamine and it receptors[J].Acta Entomologica Sinica,56(11):1342-1358
Xu H D,Wang J S,Li M H,et al.2015.1HNMR based metabolomic approach to study the toxic effects of herbicide butachlor on goldfis(Carassius auratus)[J].Aquatic Toxicology,159:69-80
Zhang X T,Breksa A P,Mishchuk D O,et al.2012.Fertilisation an pesticides affect mandarin orange nutrient composition[J].Foo Chemistry 134,1020-1024
张铎,侯志广,邬美男,等.2014.氯吡嘧磺隆的水解动力学研究[J]中国农学通报,30(35):310-313
张铎.2014.氯吡嘧磺隆的水解与光解特性研究[D].长春:吉林农业大学
钟红舰,刘进玺,吴绪金,等.2011.75%氯吡嘧磺隆水分散粒剂在玉米及土壤中的消解动态与残留[J].农药,50(12):906-908