藤茶中抗氧化剂阻断N-亚硝胺合成的作用
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摘要
为了研究抗氧化剂阻断N-亚硝酸胺合成的作用,本文以藤茶为原料,通过分级提取,研究各分级相提取物对亚硝酸盐的清除作用和N-亚硝胺的生成抑制作用,结果表明乙醇相提取物的效果最好,对亚硝酸盐的清除率和亚硝胺抑制的半抑制浓度(IC50)分别为0.023μg·mL-1和0.029μg·mL-1,强于阳性对照抗坏血酸;同时各分级相对DPPH自由基、ABTS自由基的清除作用以及对铁氰化钾的还原作用的实验结果表明,乙酸乙酯相和乙醇相具有较好的抗氧化作用,其中乙酸乙酯相的抗氧化作用强于阳性对照Trolox。HPLC定量分析显示这两相中含有较高的二氢杨梅素,分别为678.5 mg·g-1和381.1 mg·g-1,表明二氢杨梅素是藤茶中主要的抗氧化以及阻断N-亚硝胺合成的活性物质。本研究表明藤茶具有较强的抗氧化及阻断N-亚硝酸胺合成的作用,为藤茶保健品的开发提供了理论依据。
In order to study the effects of antioxidant on blocking synthesis of N-nitrosamine,sequential extraction procedure was used to extract vine tea,and the effects of each phase extracts on nitrite scavenging and inhibition of Nnitrosamines was studied. The results showed that the ethanol phase extracts had the best activities on nitrite scavenging and inhibition of N-nitrosamines synthesis with the half maximal inhibitory concentration( IC50) of 0.023 μg·mL- 1and0. 029 μg·mL- 1,which was better than the positive control ascorbic acid. Moreover,the anti-oxidant effects of each phase extracts on DPPH radical and ABTS radical scavenging,reduction power were studied,the results showed that ethyl acetate and ethanol phase had excellent oxidation activities,and the antioxidant effect of the ethyl acetate phase was stronger than the positive control Trolox. Quantitative HPLC analysis showed these two phases contains a high degree of DMY with contents of 678. 5 mg·g- 1and 381. 1 mg·g- 1,respectively. DMY was the major active compounds in vine tea with antioxidant and blocking N-nitrosamines effects. This study indicate that vine tea has anti-oxidant and blocking N-nitrosamines effects,which provide theoretical basis for the development of functional foods.
In order to study the effects of antioxidant on blocking synthesis of N-nitrosamine,sequential extraction procedure was used to extract vine tea,and the effects of each phase extracts on nitrite scavenging and inhibition of Nnitrosamines was studied. The results showed that the ethanol phase extracts had the best activities on nitrite scavenging and inhibition of N-nitrosamines synthesis with the half maximal inhibitory concentration( IC50) of 0.023 μg·mL- 1and0. 029 μg·mL- 1,which was better than the positive control ascorbic acid. Moreover,the anti-oxidant effects of each phase extracts on DPPH radical and ABTS radical scavenging,reduction power were studied,the results showed that ethyl acetate and ethanol phase had excellent oxidation activities,and the antioxidant effect of the ethyl acetate phase was stronger than the positive control Trolox. Quantitative HPLC analysis showed these two phases contains a high degree of DMY with contents of 678. 5 mg·g- 1and 381. 1 mg·g- 1,respectively. DMY was the major active compounds in vine tea with antioxidant and blocking N-nitrosamines effects. This study indicate that vine tea has anti-oxidant and blocking N-nitrosamines effects,which provide theoretical basis for the development of functional foods.
引文
[1]罗祖友,付晓芳,吴谋成.藤茶的研究进展[J].食品科学,2005,26(8):513-517
[2]覃骊兰.藤茶的化学成分及药理作用研究进展[J].上海中医药杂志,2008,42(6):94-96
[3]何桂霞,裴刚,杜方麓,欧阳文,李斌.藤茶化学成分的研究[J].中国现代中药,2007,9(12):11-13
[4]Ying L,Xu P,Huang S R,Wang Y F.Antioxidant activity of bioactive compounds extracted from Ampelopsis grossedentata leaves by optimized supercritical carbon dioxide[J].Journal of Medicinal Plants Research,2011,5(17):4373-4381
[5]郑作文,谭为,周雅君,毛健.藤茶蛇葡萄素对人卵巢癌细胞和人恶性黑色素瘤细胞增殖的抑制作用[J].广西中医药,2009,32(1):46-48
[6]高倩倩,杨秀芬,欧敏.藤茶总黄酮和二氢杨梅素含药血清对肝癌HepG2细胞增殖及凋亡的影响[J].中国中药杂志,2011,36(4):500-503
[7]周春权,姚欣,陈晓明,倪峰.藤茶提取物的抗肿瘤作用研究[J].中药新药与临床药理,2011,22(6):640-642
[8]Murakami T,Miyakoshi M,Araho D,MizutanI K,Kambara T,Ikeda T,Chou W H,Inukai M,Takenaka A,Igarashi K.Hepatoprotective activity of tocha,the stems and leaves of Ampelopsis grossedentata,and ampelopsin[J].Biofactors,2004,21(1-4):175-178
[9]Cui X Y,Yang J Y,Wang J H,Wu C F.The antiinflammatory and immunostimulating activities of ampelopsis grossedentata[J].Acta Pharmacologica Sinica,2006,27:288-289
[10]Zhao L,Wang A R,Liu B G,Li G R,Zhang Z P,Chen S J.Antioxidant and cytotoxic activity of dihydromyricetin from Ampelopsis Grossedentata leaves[J].Agro Food Industry Hi-Tech,2009,20(3):14-17
[11]陈玉琼,倪德江,程倩,黄海波,孟燕,吴谋成.藤茶总黄酮及二氢杨梅素降血脂作用研究[J].茶叶科学,2007,27(3):221-225,242
[12]马俪珍,张健斌,孟培培.食品中亚硝胺类化合物的危害及控制研究进展[J].保鲜与加工,2012,12(2):1-4,45
[13]邓秀英.食品中亚硝胺类与癌及其预防(综述)[J].中国食品卫生杂志,1999,11(1):54-56
[14]余华,汤修琴,曹丽容.天然植物成分体外模拟胃液清除亚硝酸钠及阻断亚硝胺合成的研究[J].四川食品与发酵,2005,41(2):7-10
[15]刘爱文,陈忻,郑健英.荔枝核提取液对亚硝胺的抑制作用[J].食品工业科技,2003,24(12):27-29
[16]张虹,许钢,袁建耀.刘寄奴提取液对亚硝化反应的抑制作用[J].郑州粮食学院学报,2000,21(1):50-53
[17]Lee J H,Park J C,Choi J S.The antioxidant activity of Ecklonia stolonifera[J].Archives of Pharmacal Research,1996,19(3):223-227
[18]张捷,丁韩英,戚向阳,袁勇军.枯草芽孢杆菌Y-6产抗菌肽的体外抗氧化效果研究[J].核农学报,2011,25(3):518-522,558
[19]Re R,Pellegrini N,Proteggente A,Pannala A,Yang M,RiceEvans C.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radical Biology and Medicine,1999,26(9/10):1231-1237
[20]Dorman H J D,Kosar M,Kahlos K,Holm Y,Hiltunen R.Antioxidant properties and composition of aqueous extracts from Mentha species,hybrids,varieties,and cultivars[J].Journal of Agricultural and Food Chemistry,2003,51(16):4563-4569
[21]Bruningfann C S,Kaneene J B.The Effects of Nitrate,Nitrite and N-Nitroso Compounds on Human Health-a Review[J].Veterinary and Human Toxicology,1993,35(6):521-538
[22]Jurado-Sanchez B,Ballesteros E,Gallego M.Automatic screening method for the preconcentration and determination of N-nitrosamines in water[J].Talanta,2007,73(3):498-504
[23]Kono Y,Shibata H,Kodama Y,Sawa Y.The suppression of the N-nitrosating reaction by chlorogenic acid[J].The Biochemical journal,1995,312:947~953
[24]赵二劳,王晓妮,张海容,李满秀.山楂清除亚硝酸盐及阻断亚硝胺合成的研究[J].食品与发酵工业,2006,32(10):29-31
[25]傅茂润,陈庆敏,茅林春.黄花菜提取物对亚硝化反应的抑制能力研究[J].食品科学,2009,30(15):114-119
[2]覃骊兰.藤茶的化学成分及药理作用研究进展[J].上海中医药杂志,2008,42(6):94-96
[3]何桂霞,裴刚,杜方麓,欧阳文,李斌.藤茶化学成分的研究[J].中国现代中药,2007,9(12):11-13
[4]Ying L,Xu P,Huang S R,Wang Y F.Antioxidant activity of bioactive compounds extracted from Ampelopsis grossedentata leaves by optimized supercritical carbon dioxide[J].Journal of Medicinal Plants Research,2011,5(17):4373-4381
[5]郑作文,谭为,周雅君,毛健.藤茶蛇葡萄素对人卵巢癌细胞和人恶性黑色素瘤细胞增殖的抑制作用[J].广西中医药,2009,32(1):46-48
[6]高倩倩,杨秀芬,欧敏.藤茶总黄酮和二氢杨梅素含药血清对肝癌HepG2细胞增殖及凋亡的影响[J].中国中药杂志,2011,36(4):500-503
[7]周春权,姚欣,陈晓明,倪峰.藤茶提取物的抗肿瘤作用研究[J].中药新药与临床药理,2011,22(6):640-642
[8]Murakami T,Miyakoshi M,Araho D,MizutanI K,Kambara T,Ikeda T,Chou W H,Inukai M,Takenaka A,Igarashi K.Hepatoprotective activity of tocha,the stems and leaves of Ampelopsis grossedentata,and ampelopsin[J].Biofactors,2004,21(1-4):175-178
[9]Cui X Y,Yang J Y,Wang J H,Wu C F.The antiinflammatory and immunostimulating activities of ampelopsis grossedentata[J].Acta Pharmacologica Sinica,2006,27:288-289
[10]Zhao L,Wang A R,Liu B G,Li G R,Zhang Z P,Chen S J.Antioxidant and cytotoxic activity of dihydromyricetin from Ampelopsis Grossedentata leaves[J].Agro Food Industry Hi-Tech,2009,20(3):14-17
[11]陈玉琼,倪德江,程倩,黄海波,孟燕,吴谋成.藤茶总黄酮及二氢杨梅素降血脂作用研究[J].茶叶科学,2007,27(3):221-225,242
[12]马俪珍,张健斌,孟培培.食品中亚硝胺类化合物的危害及控制研究进展[J].保鲜与加工,2012,12(2):1-4,45
[13]邓秀英.食品中亚硝胺类与癌及其预防(综述)[J].中国食品卫生杂志,1999,11(1):54-56
[14]余华,汤修琴,曹丽容.天然植物成分体外模拟胃液清除亚硝酸钠及阻断亚硝胺合成的研究[J].四川食品与发酵,2005,41(2):7-10
[15]刘爱文,陈忻,郑健英.荔枝核提取液对亚硝胺的抑制作用[J].食品工业科技,2003,24(12):27-29
[16]张虹,许钢,袁建耀.刘寄奴提取液对亚硝化反应的抑制作用[J].郑州粮食学院学报,2000,21(1):50-53
[17]Lee J H,Park J C,Choi J S.The antioxidant activity of Ecklonia stolonifera[J].Archives of Pharmacal Research,1996,19(3):223-227
[18]张捷,丁韩英,戚向阳,袁勇军.枯草芽孢杆菌Y-6产抗菌肽的体外抗氧化效果研究[J].核农学报,2011,25(3):518-522,558
[19]Re R,Pellegrini N,Proteggente A,Pannala A,Yang M,RiceEvans C.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radical Biology and Medicine,1999,26(9/10):1231-1237
[20]Dorman H J D,Kosar M,Kahlos K,Holm Y,Hiltunen R.Antioxidant properties and composition of aqueous extracts from Mentha species,hybrids,varieties,and cultivars[J].Journal of Agricultural and Food Chemistry,2003,51(16):4563-4569
[21]Bruningfann C S,Kaneene J B.The Effects of Nitrate,Nitrite and N-Nitroso Compounds on Human Health-a Review[J].Veterinary and Human Toxicology,1993,35(6):521-538
[22]Jurado-Sanchez B,Ballesteros E,Gallego M.Automatic screening method for the preconcentration and determination of N-nitrosamines in water[J].Talanta,2007,73(3):498-504
[23]Kono Y,Shibata H,Kodama Y,Sawa Y.The suppression of the N-nitrosating reaction by chlorogenic acid[J].The Biochemical journal,1995,312:947~953
[24]赵二劳,王晓妮,张海容,李满秀.山楂清除亚硝酸盐及阻断亚硝胺合成的研究[J].食品与发酵工业,2006,32(10):29-31
[25]傅茂润,陈庆敏,茅林春.黄花菜提取物对亚硝化反应的抑制能力研究[J].食品科学,2009,30(15):114-119