一测多评法测定转移因子口服液中核糖核酸的含量
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摘要
目的:建立转移因子口服液中核糖核酸的一测多评含量测定方法。方法:对转移因子口服液酶解条件、色谱方法筛选,以鸟嘌呤脱氧核苷酸为内参物,建立其余核苷酸,核苷及总核糖核酸含量的一测多评法。结果:各核苷酸及核苷峰形好,分离度高,一测多评法及多对照外标法测定结果基本一致。结论:此方法实现了转移因子口服液中核糖核酸的准确定量,同时提高检测效率,降低检测成本,适用于转移因子口服液中的核糖核酸的检测与监控。
Objective:To establish a quantitative analysis of multi-components by singer-marker(QMSA)assay method for ribonucleic acid of transfer factor oral solution(TFO). Methods:Through the enzymatic hydrolysis condition of TFO and the HPLC method optimization,a QMSA assay method was established for nucleotide,nucleoside and total ribonucleic acid by using the guanine deoxynucleotide as the internal reference. Results:the peak shape of each nucleotide and nucleoside were good and the resolution was high. The result of QMSA method and multi-control external standard method were basically the same. Conclusion:the method realizes accurate quantification of ribonucleic acid in TFO,improves the detection efficiency,reduces the detection cost. It is suitable for the detection and monitoring ribonucleic acid in TFO.
Objective:To establish a quantitative analysis of multi-components by singer-marker(QMSA)assay method for ribonucleic acid of transfer factor oral solution(TFO). Methods:Through the enzymatic hydrolysis condition of TFO and the HPLC method optimization,a QMSA assay method was established for nucleotide,nucleoside and total ribonucleic acid by using the guanine deoxynucleotide as the internal reference. Results:the peak shape of each nucleotide and nucleoside were good and the resolution was high. The result of QMSA method and multi-control external standard method were basically the same. Conclusion:the method realizes accurate quantification of ribonucleic acid in TFO,improves the detection efficiency,reduces the detection cost. It is suitable for the detection and monitoring ribonucleic acid in TFO.
引文
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[6]Hua J,Huang KL. Optimization by orthogonal array design of ion-pair HPLC separation of the enzymatic hydrolysis products of yeast RNA[J]. Chin Chem Lett,2010,21(12):1453-1456.
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[8]叶明立,潘广文,胡忠阳,等.离子色谱法测定婴幼儿配方奶粉中的核苷酸[J].色谱,2010,28(9):898-901.
[2]Wang JF,Park AJ,Rendini T,Levis WR. Lawrence transfer factor:transference of specific immune memory by dialyzable leukocyte extract from a CD8+T cell line[J]. J Drugs Dermatol,2017,16(12):1198-1206.
[3]国家药品标准·地标升国标·第十六册[S].2000:97,330.
[4]国家药品标准·地标升国标·第九册[S].2002:126.
[5]张再平,吴小曼,石蓓佳,等.反相离子对色谱法测定注射用核糖核酸酶降解产物中的5`-核苷酸[J].中南药学,2013,11(3):194-198.
[6]Hua J,Huang KL. Optimization by orthogonal array design of ion-pair HPLC separation of the enzymatic hydrolysis products of yeast RNA[J]. Chin Chem Lett,2010,21(12):1453-1456.
[7]徐彬,张仕华.反相离子对色谱法测定奶粉中核苷酸质量浓度[J].中国乳品工业,2012,40(4):45-47.
[8]叶明立,潘广文,胡忠阳,等.离子色谱法测定婴幼儿配方奶粉中的核苷酸[J].色谱,2010,28(9):898-901.