干旱胁迫对山定子水分生理及AQPs表达的影响
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摘要
【目的】研究干旱胁迫对山定子水分生理及AQPs表达水平的影响.【方法】2年生山定子盆栽苗,设置对照、轻度干旱胁迫、中度干旱胁迫和重度干旱胁迫4个试验处理.【结果】山定子叶片水势、叶片含水量、自由水、叶片相对含水量和茎、根系组织含水随干旱胁迫程度的加重而显著降低,束缚水含量及饱和亏则显著增加,复水后各水分生理呈恢复趋势.干旱胁迫影响AQPs的表达,MdPIP2;4,MdNIP4;2和MdNIP6;1在山定子叶片中随干旱胁迫程度的变化其表达量均上调,MdPIP1;4在叶片中未表达,其他AQPs均呈下调趋势.AQPs在山定子根中全部表达,其中MdNIP4;1,MdTIP1;3,MdTIP2;2和MdTIP5;1在山定子根部随胁迫程度的变化其表达量均上调,MdNIP2;2和Mdδ-TIP表达量下调.【结论】干旱胁迫能够显著影响山定子叶片含水量、自由水、叶片水势等水分生理的变化,基质水分与各水分生理呈相关或显著相关性.AQPs的表达受干旱胁迫的显著影响,在山定子叶片和根部的表达存在组织差异性,主要表现为AQPs表达量的显著差异.
To understand the effects of drought stress on water physiology and AQPs expression of Malus baccata.【Method】Two years old potted seedlings were used as materials.The treatments were designed as the control,mild,moderate and severe drought stress.【Result】The leaf water potential,leaf water content,free water,relative water content of leaves and stem and root tissue water content were significantly decreased with the increase of drought stress,while the bound water content and saturation deficit were significantly increased,and the water physiological tended to recover after rewatering.The expression of MdPIP2;4,MdNIP4;2 and MdNIP6;1 were up-regulated in the leaves,MdPIP1;4 was not expressed in the leaves,and the other AQPs were down-regulated.The expression levels of MdNIP4;1,MdTIP1;3,MdTIP2;2 and MdTIP5;1 in the roots were all up-regulated,and the expression levels of MdNIP2;2 and Mdδ-TIP were down-regulated.【Conclusions】Drought stress significantly affect the changes of water physiology of the M.baccata,and there is a significant correlation between matrix moisture and each water physiology.The expression of AQPs is significantly affected by drought stress,and there is a significant difference in the expression of AQPs in leaves and roots.
To understand the effects of drought stress on water physiology and AQPs expression of Malus baccata.【Method】Two years old potted seedlings were used as materials.The treatments were designed as the control,mild,moderate and severe drought stress.【Result】The leaf water potential,leaf water content,free water,relative water content of leaves and stem and root tissue water content were significantly decreased with the increase of drought stress,while the bound water content and saturation deficit were significantly increased,and the water physiological tended to recover after rewatering.The expression of MdPIP2;4,MdNIP4;2 and MdNIP6;1 were up-regulated in the leaves,MdPIP1;4 was not expressed in the leaves,and the other AQPs were down-regulated.The expression levels of MdNIP4;1,MdTIP1;3,MdTIP2;2 and MdTIP5;1 in the roots were all up-regulated,and the expression levels of MdNIP2;2 and Mdδ-TIP were down-regulated.【Conclusions】Drought stress significantly affect the changes of water physiology of the M.baccata,and there is a significant correlation between matrix moisture and each water physiology.The expression of AQPs is significantly affected by drought stress,and there is a significant difference in the expression of AQPs in leaves and roots.
引文
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[22]张红萍,牛俊义,轩春香,等.干旱胁迫及复水对豌豆叶片脯氨酸和丙二醛含量的影响[J].甘肃农业大学学报,2008,43(5):50-54.
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[24]CASTEL J R,FERERES E.Responses of young almond trees to two drought periods in the field[J].Journal of Horticultural Science,1982,57(2):175-187.
[25]彭守兰,曾凡江,刘波,等.极端干旱条件下策勒绿洲引种植物水分生理特征研究[J].干旱区地理,2013,36(3):457-466
[26]杨朝选,焦国利,王新峰,等.干旱过程中桃树茎和叶水势的变化[J].果树科学,1999(4):267-271.
[27]韩晓毓.不同苹果砧木导水特性与水通道蛋白基因表达对干旱与盐胁迫的响应[D].杨凌:西北农林科技大学,2015.
[28]王雷宏.山荆子(Malus baccata(L.)Borkh.)变异式样研究[D].南京:南京林业大学,2008.
[29]CHAUMONT F,TYERMAN S D.Aquaporins:highly regulated channels controlling plant water relations[J].Plant Physiology,2014,164(4):1600-1618.
[30]WALLACE I S,WILLS D M,GUENTHER J F.Functional selectivity for glycerol of the nodulin 26subfamily of plant membrane intrinsic proteins[J].FEBS letters,2002,523(1-3):109-112.
[31]ALEXANDERSSON E,FRAYSSE L,SJVALLLARSEN S,et al.Whole gene family expression and drought stress regulation of aquaporins[J].Plant molecular biology,2005,59(3):469-484.
[2]FAROOQ M,WAHID A,KOBAYASHI N,et al.Plant drought stress:effects,mechanisms and management[J].Agronomy for Sustainable Development,2009:29(1):185-212.
[3]ROMERO P,NAVARRO J M,PREZ-PREZ J.Deficit irrigation and rootstock:their effects on water relations,vegetative development,yield,fruit quality and mineral nutrition of Clemenules mandarin[J].Tree Physiology,2006,26(12):1537-1548.
[4]SCHUURMANS J A M J,VAN DONGEN J T,RUTJENS B P W.Members of the aquaporin family in the developing pea seed coat include representatives of the PIP,TIP,and NIP subfamilies[J].Plant molecular biology,2003,53(5):655-667.
[5]LOVISOLO C,SCHUBERT A.Mercury hinders recovery of shoot hydraulic conductivity du-ring grapevine rehydration:evidence from a whole-plant approach[J].New Phytologist,2006,172(3):469-478.
[6]YIN Y X,WANG S B,ZHANG H X,et al.Cloning and expression analysis of CaPIP1-1gene in pepper(Capsicum annuum L.)[J].Gene,2015,563(1):87-93.
[7]WEI Z,HU W,LIN Q,et al.Understanding rice plant resistance to the Brown Planthopper(Nilaparvata lugens):aproteomic approach[J].Proteomics,2009,9(10):2798-2808.
[8]ARNDT S K,CLIFFORD S C,WANEK W,et al.Physiological and morphological adaptations of the fruit tree Ziziphus rotundifolia in response to progressive drought stress[J].Tree Physiology,2001,21(11):705-715.
[9]IRCELJ H,TAUSZ M,GRILL D,et al.Biochemical responses in leaves of two apple tree cultivars subjected to progressing drought[J].Journal of plant physiology,2005,162(12):1308-1318.
[10]徐启贺.苹果砧木对干旱胁迫的生理响应及抗旱性评价[D].北京:中国农业科学院,2010.
[11]张栋.干旱胁迫对苹果光合作用和叶绿素荧光的影响及叶片衰老特性研究[D].杨凌:西北农林科技大学,2011.
[12]MINGEAU M,PERRIER C,AMGLIO T.Evidence of drought-sensitive periods from flowering to maturity on highbush blueberry[J].Scientia Horticulturae,2001,89(1):23-40.
[13]肖玉明.干旱胁迫对温州蜜柑果实品质及柠檬酸代谢相关基因表达的影响[D].长沙:湖南农业大学,2014.
[14]陈立松,刘星辉.果树对水分胁迫的反应与适应性[J].干旱地区农业研究,1999(1):91-97
[15]LIU C,LI C,LIANG D.Aquaporin expression in response to water-deficit stress in two Malus species:relationship with physiological status and drought tolerance[J].Plant Growth Regulation,2013,70(2):187-197.
[16]HU C G,HAO Y J,HONDA C.Putative PIP1genes isolated from apple:expression analyses during fruit development and under osmotic stress[J].Journal of experimental botany,2003,54(390):2193-2194.
[17]刘向莉,高丽红,刘明池.植物组织中自由水和束缚水含量测定方法的改进[J].中国蔬菜,2005(4):9-11.
[18]陈林,杨新国,宋乃平,等.干旱半干旱地区植物叶片水分吸收性状[J].浙江大学学报(农业与生命科学版),2013(5):565-574.
[19]王艺陶,周宇飞,李丰先,等.干旱胁迫下高粱叶温与叶片水分状况的关系[J].干旱地区农业研究,2013(6):146-151.
[20]徐扬,赵健,张雷,等.干旱胁迫对板栗二年生嫁接苗叶片相对含水量和可溶性糖含量的影响[J].农学学报,2017,7(1):91-94.
[21]朱新强,张新颖,师尚礼,等.干旱胁迫下4个苜蓿品种根系抗旱性的比较[J].甘肃农业大学学报,2012,47(1):103-107.
[22]张红萍,牛俊义,轩春香,等.干旱胁迫及复水对豌豆叶片脯氨酸和丙二醛含量的影响[J].甘肃农业大学学报,2008,43(5):50-54.
[23]山东农学院,西北农学院.植物生理学实验指导[M].济南:山东科学技术出版社,1982:121-128.
[24]CASTEL J R,FERERES E.Responses of young almond trees to two drought periods in the field[J].Journal of Horticultural Science,1982,57(2):175-187.
[25]彭守兰,曾凡江,刘波,等.极端干旱条件下策勒绿洲引种植物水分生理特征研究[J].干旱区地理,2013,36(3):457-466
[26]杨朝选,焦国利,王新峰,等.干旱过程中桃树茎和叶水势的变化[J].果树科学,1999(4):267-271.
[27]韩晓毓.不同苹果砧木导水特性与水通道蛋白基因表达对干旱与盐胁迫的响应[D].杨凌:西北农林科技大学,2015.
[28]王雷宏.山荆子(Malus baccata(L.)Borkh.)变异式样研究[D].南京:南京林业大学,2008.
[29]CHAUMONT F,TYERMAN S D.Aquaporins:highly regulated channels controlling plant water relations[J].Plant Physiology,2014,164(4):1600-1618.
[30]WALLACE I S,WILLS D M,GUENTHER J F.Functional selectivity for glycerol of the nodulin 26subfamily of plant membrane intrinsic proteins[J].FEBS letters,2002,523(1-3):109-112.
[31]ALEXANDERSSON E,FRAYSSE L,SJVALLLARSEN S,et al.Whole gene family expression and drought stress regulation of aquaporins[J].Plant molecular biology,2005,59(3):469-484.