苦木水提物外用对变应性接触性皮炎模型小鼠的改善作用研究
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摘要
目的:考察苦木水提物外用对变应性接触性皮炎(ACD)模型小鼠的改善作用,为其进一步开发利用提供参考。方法:将48只小鼠随机分为空白对照组(生理盐水)、模型组(生理盐水)、氢化可的松组(阳性对照,约0.15 g/耳)和苦木水提物低、中、高剂量组(0.15、0.3、0.6 g/耳,以提取物计),每组8只。耳部涂抹给药,每天上午8点和下午4点分别给药1次,连续给药7 d。除空白对照组外,其余各组小鼠在第1、2天给药后于腹部涂抹含0.5%2,4-二硝基氟苯(DNFB)的丙酮-橄榄油溶液诱导ACD模型。第7天给药结束后,于造模组小鼠左耳涂抹0.25%DNFB的丙酮-橄榄油液诱发皮炎。24 h后,肉眼观察各组小鼠左耳组织变化并进行皮损评分,测定小鼠左、右耳厚度差和质量差,检测小鼠血清中免疫球蛋白E(IgE)和白细胞介素6(IL-6)含量,并在苏木精-伊红染色后于显微镜下观察小鼠耳组织病理形态学变化。结果:与空白对照组比较,模型组小鼠左耳皮损评分显著升高(P<0.01),左、右耳厚度差和质量差以及血清中IgE、IL-6含量均显著增加(P<0.01),显微镜下可见小鼠左耳组织出现淋巴细胞浸润、细胞间水肿、红细胞渗出等病理形态学变化。与模型组比较,氢化可的松组和苦木水提物高剂量组小鼠左耳皮损评分显著降低(P<0.05或P<0.01),各给药组小鼠左、右耳厚度差和质量差均显著减小(P<0.01),氢化可的松组和苦木水提物中、高剂量组小鼠血清中IgE、IL-6含量均显著降低(P<0.01),显微镜下可见各给药组小鼠左耳炎性病变均不同程度减轻。结论:苦木水提物外用对ACD模型小鼠具有较好的改善作用。
OBJECTIVE:To investigate the improvement effects of external application of water extract from Picrasma quassioides on allergic contact dermatitis(ACD)model mice,and to provide reference for its further development and utilization.METHODS:A total of 48 mice were randomly divided into blank control group(normal saline),model group(normal saline),hydrocortisone group(positive control,about 0.15 g/ear) and water extract from P. quassioides low-dose,medium-dose and high-dose groups(0.15,0.3,0.6 g/ear, calculated by extract),with 8 mice in each group. The mice were given medicine on ear at8 a.m. and 4 p.m. for 7 d continuously. Except for blank control group,other groups were given acetone-olive oil solution containing 0.5% 2,4-dinitrofluorobenzene(DNFB)on the abdomen of mice to induce ACD model. After 7 d medication,modeling group was given acetone-olive oil of 0.25% DNFB on left ear to induce dermatitis. 24 h later,the pathological changes of left ear tissue were observed by naked eye and skin lesion was scored. The thickness difference and weight difference of left and right ears in mice were measured. The serum contents of IL-6 and IgE were determined. The pathomorphology changes of ear tissue were observed by microscope after HE staining. RESULTS:Compared with blank control group,the skin lesion score of left ear was increased significantly in model group(P<0.01);the thickness difference and weight difference of left and right ears,serum contents of IgE and IL-6 were increased significantly(P<0.01). Pathological changes were observed in left tissue of mice by microscope,such as lymphocytic infiltration,intercellular edema,erythrocyte exudation. Compared with model group,skin lesion score of left ear in mice was decreased significantly in hydrocortisone group and water extract from P. quassioides high-dose group(P<0.05 or P<0.01);the thickness difference and weight difference of left and right ears in mice were decreased significantly in all administration groups(P<0.01). The serum contents of IgE and IL-6 in mice were decreased significantly in hydrocortisone group,water extract from P. quassioides medium-dose and high-dose groups(P<0.01);the inflammatory lesions of left ear in mice were alleviated to varying degrees in different administration groups. CONCLUSIONS:External application of water extract from P. quassioides has a good improvement effect on ACD model mice.
OBJECTIVE:To investigate the improvement effects of external application of water extract from Picrasma quassioides on allergic contact dermatitis(ACD)model mice,and to provide reference for its further development and utilization.METHODS:A total of 48 mice were randomly divided into blank control group(normal saline),model group(normal saline),hydrocortisone group(positive control,about 0.15 g/ear) and water extract from P. quassioides low-dose,medium-dose and high-dose groups(0.15,0.3,0.6 g/ear, calculated by extract),with 8 mice in each group. The mice were given medicine on ear at8 a.m. and 4 p.m. for 7 d continuously. Except for blank control group,other groups were given acetone-olive oil solution containing 0.5% 2,4-dinitrofluorobenzene(DNFB)on the abdomen of mice to induce ACD model. After 7 d medication,modeling group was given acetone-olive oil of 0.25% DNFB on left ear to induce dermatitis. 24 h later,the pathological changes of left ear tissue were observed by naked eye and skin lesion was scored. The thickness difference and weight difference of left and right ears in mice were measured. The serum contents of IL-6 and IgE were determined. The pathomorphology changes of ear tissue were observed by microscope after HE staining. RESULTS:Compared with blank control group,the skin lesion score of left ear was increased significantly in model group(P<0.01);the thickness difference and weight difference of left and right ears,serum contents of IgE and IL-6 were increased significantly(P<0.01). Pathological changes were observed in left tissue of mice by microscope,such as lymphocytic infiltration,intercellular edema,erythrocyte exudation. Compared with model group,skin lesion score of left ear in mice was decreased significantly in hydrocortisone group and water extract from P. quassioides high-dose group(P<0.05 or P<0.01);the thickness difference and weight difference of left and right ears in mice were decreased significantly in all administration groups(P<0.01). The serum contents of IgE and IL-6 in mice were decreased significantly in hydrocortisone group,water extract from P. quassioides medium-dose and high-dose groups(P<0.01);the inflammatory lesions of left ear in mice were alleviated to varying degrees in different administration groups. CONCLUSIONS:External application of water extract from P. quassioides has a good improvement effect on ACD model mice.
引文
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[2]阮毅铭,梅全喜,关健缨,等.漆大姑水提物外用对慢性皮炎-湿疹模型小鼠的改善作用研究[J].中国药房,2018,29(11):1536-1541.
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[21]胡威,马源源,宋伟.龙珠软膏对变应性接触性皮炎作用研究[J].中国药师,2017,20(8):1360-1363.
[22]胡梦颖,冷英莉,刘晓燕.百癣夏塔热片对模型小鼠变应性接触性皮炎的抑制作用[J].中国药房,2015,26(22):3052-3054.
[23]周洪莉,阙昌田,王学,等.荆防散乙酸乙酯萃取部位及分离物的抗过敏作用研究[J].中药材,2017,40(9):2182-2186.
[24]吴瑜丹,曾丽萍,陶爱林,等.瘙痒介质在小鼠变应性接触性皮炎模型背根神经节中的表达[J].中国皮肤性病学杂志,2017,31(7):727-731.
[25]谷冬梅,李会强.由IgE介导的(速发型)芒果过敏及其过敏原的研究进展[J].中国免疫学杂志,2015,31(6):849-851.
[26]NAVI D,SAEGUSA J,LIU FT,et al.Mast cells and immunological skin diseases[J].Clin Rev Allergy Immunol,2007,33(1/2):144-155.
[27]肖作奇,欧阳波,肖素希,等.阴炎净洗剂对大鼠接触性皮炎湿疹的防治作用及机制研究[J].中国药房,2017,28(28):3931-3934.
[2]阮毅铭,梅全喜,关健缨,等.漆大姑水提物外用对慢性皮炎-湿疹模型小鼠的改善作用研究[J].中国药房,2018,29(11):1536-1541.
[3]夏月平.中医药治疗湿疹的临床研究进展[J].临床医药文献杂志,2016,3(23):4726-4727.
[4]陈平,杨志波,杨光艳,等.外用中药治疗慢性湿疹研究进展[J].中华中医药学刊,2012,30(3):523-526.
[5]黄礴,吴铁,樊翌明.6种中药抑制二硝基氟苯所致小鼠接触性皮炎的实验研究[J].现代中西医结合杂志,2007,16(13):1756-1757.
[6]李中辉,魏跃钢.中药大青叶、黄柏、龙胆草对小鼠皮肤炎症反应的影响[J].中国麻风皮肤病杂志,2015,31(5):288-291.
[7]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:200.
[8]ZHAO WN,SUN C,HE J,et al.The possible mechanisms of Picrasma quassiodes(D.Don)Benn.in the treatment of colitis induced by 2,4,6-trinitrobenzene sulfonic acid in mice[J].J Ethnopharmacol,2013,145(2):424-430.
[9]宋振玉,周同,方起程.中草药现代研究[M].北京:北京医科大学和中国协和医科大学联合出版社,1996:468-469.
[10]何颖,刘伟,陈忠伟,等.苦木生物碱体外抑制大肠杆菌效果的研究[J].安徽农业科学,2008,36(7):2777-2778.
[11]杨俊山,罗淑荣,沈秀兰,等.苦木生物碱的化学研究[J].药学学报,1979,14(3):167-177.
[12]YU Y,SEOK KL,MYEONG-HYEON W.Isolation and biological activities of an alkaloid compound(3-methylcanthin-5,6-dione)from Picrasma quassiodes(D.Don)Benn[J].Nat Prod Sci,2011,17(1):5-9.
[13]JAE WL,JI WP,SHIN NR,et al.Picrasma quassiodes(D.Don)Benn.attenuates lipopolysaccharide(LPS)induced acute lung injury[J].Int J Mol Med,2016,38(3):834-844.
[14]SHIN NR,SHIN IS,JEON CM,et al.Inhibitory effects of Picrasma quassioides(D.Don)Benn.on airway inflammation in a murine model of allergic asthma[J].Mol Med Rep,2014,10(3):1495-1500.
[15]芦源,关洪全.养血消风饮对小鼠皮炎-湿疹模型的影响[J].中医学报,2015,30(5):700-702.
[16]赵辨.湿疹面积及严重度指数评分法[J].中华皮肤科杂志,2004,37(1):3-4.
[17]WULLERT A,BONNET MC,PASPARAKIS M.NF-κBin the regulation of epithelial homeostasis and inflammation[J].Cell Res,2011,21(1):146-158.
[18]沈琪,王梅,康金森.沙棘油对小鼠变应性接触性皮炎的治疗作用[J].中国比较医学杂志,2017,27(11):50-55.
[19]中华医学会皮肤性病学分会免疫学组.中国湿疹诊疗指南(2011年)[J].中华皮肤科杂志,2011,44(1):5-6.
[20]LI WH,LIU FX,WANG J,et al.MicroRNA-21-mediated inhibition of mast cell degranulation involved in the protective effect of berberine on 2,4-dinitrofluorobenzene induced allergic contact dermatitis in rats via p38 pathway[J].Inflammation,2018,41(2):689-699.
[21]胡威,马源源,宋伟.龙珠软膏对变应性接触性皮炎作用研究[J].中国药师,2017,20(8):1360-1363.
[22]胡梦颖,冷英莉,刘晓燕.百癣夏塔热片对模型小鼠变应性接触性皮炎的抑制作用[J].中国药房,2015,26(22):3052-3054.
[23]周洪莉,阙昌田,王学,等.荆防散乙酸乙酯萃取部位及分离物的抗过敏作用研究[J].中药材,2017,40(9):2182-2186.
[24]吴瑜丹,曾丽萍,陶爱林,等.瘙痒介质在小鼠变应性接触性皮炎模型背根神经节中的表达[J].中国皮肤性病学杂志,2017,31(7):727-731.
[25]谷冬梅,李会强.由IgE介导的(速发型)芒果过敏及其过敏原的研究进展[J].中国免疫学杂志,2015,31(6):849-851.
[26]NAVI D,SAEGUSA J,LIU FT,et al.Mast cells and immunological skin diseases[J].Clin Rev Allergy Immunol,2007,33(1/2):144-155.
[27]肖作奇,欧阳波,肖素希,等.阴炎净洗剂对大鼠接触性皮炎湿疹的防治作用及机制研究[J].中国药房,2017,28(28):3931-3934.