HPLC法同时测定石龙清血颗粒中4种成分的含量
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摘要
目的建立高效液相色谱法(HPLC)同时测定石龙清血颗粒中没食子酸、原儿茶酸、马钱苷、芦丁的含量。方法采用高效液相色谱法,Kromasil C_(18)(250 mm×460 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸水(B)为流动相进行梯度洗脱,流速为1.0 mL·min~(-1),柱温为30℃,检测波长为240 nm。结果石龙清血颗粒中没食子酸、原儿茶酸、马钱苷、芦丁的线性范围分别为33.4～100.2、15.0～44.8、82.0～244.9、450.0～1343.7 mg·L~(-1),相关系数r均大于等于0.999 0,加样回收率均符合要求,含量分别为0.6577、0.2662、1.195和6.652 mg·g~(-1),含量RSD分别为0.9%、1.0%、1.4%和1.2%。阴性对照无干扰,各峰分离度良好。结论所建立的方法适用于石龙清血颗粒的质量控制。
Objective To establish a high performance liquid chromatography(HPLC) method to determinate the content in Shilongqingxue Granules(gallic acid,protocatechuic acid,loganin and rutin).Methods The column was Kromasil C_(18)(250 mm × 4.6 mm,5μm):acetonitrile(A)-0.1% phosphoric acid(B) was used as mobile phase in gradient elution:the flow rate was 1.0 mL·min~(-1):column temperature was 30 °C:the detection wavelength was 240 nm.Results The linear ranges of gallic acid,protocatechuic acid,loganin and rutin in Shilongqingxue Granule were 33.4-100.2、15.0-44.8、82.0-244.9、450.0-1 343.7 mg·L~(-1),respectively. The correlation coefficient r was greater than or equal to 0.999 0. The recovery rates of the samples were all between 95% and 105%:the contents were 0.6576,0.266 2,1.195,and 6.652 mg·g~(-1),respectively,and the RSD were 0.9%、1.0%、1.4% and 1.2%,respectively. The negative control had no interference and the peaks were well separated.Conclusion This method is applicable to the quality control of Shilongqingxue granules.
Objective To establish a high performance liquid chromatography(HPLC) method to determinate the content in Shilongqingxue Granules(gallic acid,protocatechuic acid,loganin and rutin).Methods The column was Kromasil C_(18)(250 mm × 4.6 mm,5μm):acetonitrile(A)-0.1% phosphoric acid(B) was used as mobile phase in gradient elution:the flow rate was 1.0 mL·min~(-1):column temperature was 30 °C:the detection wavelength was 240 nm.Results The linear ranges of gallic acid,protocatechuic acid,loganin and rutin in Shilongqingxue Granule were 33.4-100.2、15.0-44.8、82.0-244.9、450.0-1 343.7 mg·L~(-1),respectively. The correlation coefficient r was greater than or equal to 0.999 0. The recovery rates of the samples were all between 95% and 105%:the contents were 0.6576,0.266 2,1.195,and 6.652 mg·g~(-1),respectively,and the RSD were 0.9%、1.0%、1.4% and 1.2%,respectively. The negative control had no interference and the peaks were well separated.Conclusion This method is applicable to the quality control of Shilongqingxue granules.
引文
[1] 曹德万,李殿芝.超声波提取法测定石龙清血颗粒含量研究[J].亚太传统医药,2013,24 (5):54-55.
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[8] 皮胜玲,张凯强,胡玉珍,等.野生与栽培夏枯草5 种活性成分的HPLC 测定[J].中草药,2017,48(8):1666-1670.
[9] 楼招欢,吕圭源,颜美秋,等.HPLC法测定复方夏枯草片中绿原酸的含量[J].中华中医药学刊,2011,29(6):1250-1252.
[10] 冯伟红,李春,信伟梅,等.生物测定法用于中药质量评价的探索研究——以夏枯草抗氧化活性与总酚酸含量相关性的研究为例[J].中国中药杂志,2016,16(14):2660-2668.
[11] 程琛舒.山茱萸化学成分的研究[D].安徽大学,安徽合肥:2011.
[12] 高意,周光明,陈军华,等 .高效液相色谱法同时测定仙鹤草中6种黄酮[J].食品科学,2015,36(18):93-96.
[2] 左春林.刺槐花多酚提取纯化及抗氧化性研究[D].山东师范大学,山东青岛,2013.
[3] 丁飞.药用植物仙鹤草规范化栽培技术[J].现代农业,2010,10 (9):12.
[4] 刘红霞,刘召喜,姜清华,等.仙鹤草的酚类化学成分[J].沈阳药科大学学报,2010,17(4):286-289,298.
[5] 曹岗,邵玉蓝,张云,等.中药山茱萸指纹图谱的研究进展[J].中国新药杂志,2009,18(20):1950-1953.
[6] 皮文霞,许桂玲,郭戎,等.山茱萸HPLC指纹图谱研究[J].中药材,2010,33(4):530-532.
[7] 杨宁,刘艳妮,康杰芳.山茱萸药材HPLC指纹图谱的优化研究[J].药物分析杂志,2014,(11):1989-1994.
[8] 皮胜玲,张凯强,胡玉珍,等.野生与栽培夏枯草5 种活性成分的HPLC 测定[J].中草药,2017,48(8):1666-1670.
[9] 楼招欢,吕圭源,颜美秋,等.HPLC法测定复方夏枯草片中绿原酸的含量[J].中华中医药学刊,2011,29(6):1250-1252.
[10] 冯伟红,李春,信伟梅,等.生物测定法用于中药质量评价的探索研究——以夏枯草抗氧化活性与总酚酸含量相关性的研究为例[J].中国中药杂志,2016,16(14):2660-2668.
[11] 程琛舒.山茱萸化学成分的研究[D].安徽大学,安徽合肥:2011.
[12] 高意,周光明,陈军华,等 .高效液相色谱法同时测定仙鹤草中6种黄酮[J].食品科学,2015,36(18):93-96.