昆仑雪菊水提取物对小鼠抗疲劳作用的研究
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摘要
目的:研究昆仑雪菊对小鼠的抗疲劳及抗氧化作用。方法:昆明雄性小鼠40只,随机分为昆仑雪菊低剂量组(0.2 g/kg)、中剂量组(0.4 g/kg)、高剂量组(0.8 g/kg)和对照组(蒸馏水组)4组,连续灌胃30 d,观察小鼠疲劳转棒时间、跑步机电击次数;测定血浆中SOD、GSH活性及MDA、血乳酸、血尿素氮的含量。结果:昆仑雪菊能明显延长小鼠疲劳转棒时间、减少跑步机电击次数,提高血清中SOD和GSH-Px的活力,降低MDA、乳酸和血清尿素氮含量。结论:昆仑雪菊具有抗疲劳及抗氧化功作用,其作用可能是通过减少乳酸、血尿素氮生成和提高抗氧化酶活性实现的。
Objective: To study the anti-fatigue and anti-oxidation effect of Kunlun snow chrysanthemum on mice. Methods: Forty Kunming male mice were randomly divided into low dose group(0.2 g/kg), medium dose(0.4 g/kg), high dose(0.8 g/kg) and control group(distilled water group). The mice in each group were given gavage for 30 days. The weight, time of fatigue turn bar, number of shocks to the treadmill were observed, the contents of SOD, MDA, GSH, blood lactic acid, and blood urea nitrogen of mice were determined. Results: Kunlun snow chrysanthemum could significantly prolong the time of mice fatigue turn bar, reduce the number of running electromechanical hits, increase the activity of SOD and GSH in serum. It also decreased the content of MDA, lactic acid and serum urea nitrogen. Conclusion: The Kunlun snow chrysanthemum had anti-fatigue and anti-oxidative effects, which may be achieved by reducing the production of lactic acid, blood urea nitrogen and enhancing the activity of antioxidant enzymes.
Objective: To study the anti-fatigue and anti-oxidation effect of Kunlun snow chrysanthemum on mice. Methods: Forty Kunming male mice were randomly divided into low dose group(0.2 g/kg), medium dose(0.4 g/kg), high dose(0.8 g/kg) and control group(distilled water group). The mice in each group were given gavage for 30 days. The weight, time of fatigue turn bar, number of shocks to the treadmill were observed, the contents of SOD, MDA, GSH, blood lactic acid, and blood urea nitrogen of mice were determined. Results: Kunlun snow chrysanthemum could significantly prolong the time of mice fatigue turn bar, reduce the number of running electromechanical hits, increase the activity of SOD and GSH in serum. It also decreased the content of MDA, lactic acid and serum urea nitrogen. Conclusion: The Kunlun snow chrysanthemum had anti-fatigue and anti-oxidative effects, which may be achieved by reducing the production of lactic acid, blood urea nitrogen and enhancing the activity of antioxidant enzymes.
引文
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[2]远辉,孙蕾,杨文菊.新疆不同产地雪菊中氨基酸的测定及分析[J].食品科技,2015,40(07):326-329
[3]木合塔尔·吐尔洪,买买提·吐尔逊,热萨莱提·伊敏,等.RPHPLC法同时测定昆仑雪菊中绿原酸、芦丁、槲皮素和木犀草素的量[J].中草药,2016,47(09):1601-1604
[4]过利敏,张平,张谦,等.雪菊化学成分分析、提取、鉴定及其生物活性研究进展[J].食品科学,2014,35(07):298-304
[5]崔康康.新疆昆仑雪菊水提液对大鼠血脂与血压的影响研究[D].乌鲁木齐:新疆农业大学,2013
[6]崔康康,姬凤彩,王志琴,等.新疆昆仑雪菊水提液对大鼠血脂的影响[J].新疆农业大学学报,2013,36(05):366-370
[7]樊利娜,张鑫,南星梅,等.昆仑雪菊对四氧嘧啶致糖尿病小鼠糖脂代谢的影响[J].中药材,2013,36(12):2011-2013
[8]张朝霞,陈湘宏,王花,等.青海昆仑雪菊对糖尿病大鼠血糖的影响及可能机制[J].中国老年学杂志,2017,37(23):5782-5784
[9]徐斌,王丹,葛红娟,等.昆仑雪菊黄酮提取物体外抗氧化活性试验[J].中国兽医杂志,2015,51(12):103-106
[10]杜鹃,吴津蓉,吴忠红.不同产地昆仑雪菊抗氧化活性比较研究[J].食品研究与开发,2016,37(19):13-16
[11]郑大成,木合布力·阿布力孜,阿依努尔·吐鲁洪,等.昆仑雪菊水溶性黄酮的制备及初步鉴定[J].亚太传统医药,2010,10(6):18-20
[12]金亚香,张研,刘天戟,等.茶树菇提取物抗疲劳及耐缺氧作用研究[J].时珍国医国药,2016,27(3):608-611
[13]张爽,蔡璐,潘雪,等.复方人参合剂对小鼠抗疲劳和耐缺氧能力的影响[J].人参研究,2015,(02):08-10