苏打盐碱胁迫对紫花苜蓿叶片渗透调节和膜透性的影响
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摘要
为了探究苏打盐碱对紫花苜蓿幼苗叶片渗透调节物质和膜透性物质累积的影响,采用不同浓度的苏打盐碱对紫花苜蓿幼苗进行胁迫处理,测定苜蓿叶片的可溶性糖、可溶性蛋白和脯氨酸、MDA含量以及相对电导率的变化.结果表明,可溶性糖、可溶性蛋白和脯氨酸含量均随苏打盐碱浓度的增加呈先升高后降低的变化趋势,在150mmol·L~(-1)处理组达到最高,MDA含量和相对电导率随苏打盐碱浓度的增加呈逐渐上升的变化趋势,在250mmol·L~(-1)处理组达到最高.隶属函数综合分析,苏打盐碱浓度在150mmol·L~(-1)时,渗透调节物质大量积累,生物膜损伤最小.
In order to explore the effects of soda saline-alkali on osmotic adjustment substances and membrane permeability substances accumulation in Medicago sativa seedlings,the experiment was conducted to stress Medicago sativa seedlings with different concentrations of soda saline-alkali,and to determine the changes of soluble sugar,soluble protein,proline content,MDA content and relative conductivity in alfalfa leaves.The results showed that the contents of soluble sugar,soluble protein and proline increased firstly and then decreased with the increase of soda saline-alkali concentration,and reached the highest level in 150 mmol·L~(-1) treatment group.The content of MDA and relative conductivity increased gradually with the increase of soda concentration,and reached the highest level in 250 mmol·L~(-1) treatment group.According to the comprehensive analysis of membership function,when the soda saline-alkali concentration was 150 mmol·L~(-1),osmotic adjustment substances accumulated in large quantities and biofilm damage was the smallest.
In order to explore the effects of soda saline-alkali on osmotic adjustment substances and membrane permeability substances accumulation in Medicago sativa seedlings,the experiment was conducted to stress Medicago sativa seedlings with different concentrations of soda saline-alkali,and to determine the changes of soluble sugar,soluble protein,proline content,MDA content and relative conductivity in alfalfa leaves.The results showed that the contents of soluble sugar,soluble protein and proline increased firstly and then decreased with the increase of soda saline-alkali concentration,and reached the highest level in 150 mmol·L~(-1) treatment group.The content of MDA and relative conductivity increased gradually with the increase of soda concentration,and reached the highest level in 250 mmol·L~(-1) treatment group.According to the comprehensive analysis of membership function,when the soda saline-alkali concentration was 150 mmol·L~(-1),osmotic adjustment substances accumulated in large quantities and biofilm damage was the smallest.
引文
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[2]刘晓静,张晓磊,齐敏兴,等.混合盐碱对紫花苜蓿种子萌发及幼苗期叶绿素荧光特性的影响[J].草地学报,2013,21(3):501-507
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[4]雷成军,朱建兰,常永义.NaCl、Na2CO3胁迫对‘红地球’葡萄‘贝达’嫁接苗生长及生理指标的影响[J].甘肃农业大学学报,2012,47(5):50-55
[5]王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2006
[6]张治安.植物生理学实验技术[M].长春:吉林大学出版社,2008
[7]张磊,侯云鹏,王立春.盐碱胁迫对植物的影响及提高植物耐盐碱性的方法[J].东北农业科学,2018,43(4):11-16
[8]李天才,蒋海平,付梅,等.Cu2+、Hg2+及其混合胁迫对阿根廷蜈蚣草生理的影响[J].贵州农业科学,2017,45(3):18-22
[9]张晓磊,刘晓静,齐敏兴,等.混合盐碱胁迫对紫花苜蓿苗期生长特性的影响[J].草原与草坪,2013,33(1):16-20
[10]陆佳,李志辉,张斌,等.氮离子注入对仿栗种子当代生理生化性状的影响[J].核农学报,2008,22(5):617-620
[11]周丽霞,曹红星,刘艳菊.海水胁迫对椰子幼苗生理特性的影响[J].南方农业学报,2018,49(10):2013-2019
[12]覃鹏,刘叶菊,刘飞虎.干旱胁迫对烟草叶片丙二醛含量和细胞膜透性的影响[J].亚热带植物科学,2004,33(4):8-10
[13]张永锋,梁正伟,隋丽,等.盐碱胁迫对苗期紫花苜蓿生理特性的影响[J].草业学报,2009,18(4):230-235