生鲜乳中微生物检测技术研究进展
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摘要
我国现阶段乳品科技发展迅速,随之衍生一些乳品安全问题。目前,微生物引发的乳品污染问题仍然是乳品质量安全的重点和难点,生鲜乳质量安全影响着终端奶业发展,相关机构和部门都在探寻简单、便捷、廉价、高效的乳品微生物检测方法。本文就生鲜乳中微生物检测技术现状和进展进行简要综述,以供相关行业借鉴参考。
Dairy science and technology is developing rapidly at the present stage in China, then the dairy safety problems are increasingly serious, at present, the pollution problem of dairy caused by the microorganism is still the focus and difficulty in the quality and safety of dairy products, and the quality and safety of raw milk affects the development of the terminal dairy industry, related agencies and departments are exploring the methods of microbiological detection for dairy products. In this paper, the current situation and progress of microbial detection technology in raw milk were briefly reviewed, so as to provide reference for related industries.
Dairy science and technology is developing rapidly at the present stage in China, then the dairy safety problems are increasingly serious, at present, the pollution problem of dairy caused by the microorganism is still the focus and difficulty in the quality and safety of dairy products, and the quality and safety of raw milk affects the development of the terminal dairy industry, related agencies and departments are exploring the methods of microbiological detection for dairy products. In this paper, the current situation and progress of microbial detection technology in raw milk were briefly reviewed, so as to provide reference for related industries.
引文
[1]梁国添.乳品中有害微生物的检测技术和发展方向探讨[J].生物技术世界,2015,07:47.
[2]池慧芳,高丽霞,郭爱萍.浅议乳品微生物检测技术[J].农产品加工(学刊),2011,10:95-97.
[3]张爱萍,孟瑞锋,侯式娟,等.阻抗法快速分析牛奶中的菌落总数[J].中国食品学报,2012,12(02):158-164.
[4]Bancalari E,Bernini V,Bottari B,et al.Application of Impedance Microbiology for Evaluating Potential Acidifying Performances of Starter Lactic Acid Bacteria to Employ in Milk Transformation[J].Front Microbiol,2016,17(7):1628.
[5]杜寒春,叶开富,刘绍刚.电阻抗法快速测定巴氏杀菌牛乳中菌落总数[J].中国乳品工业,2017,45(4):56-61.
[6]马秋霞,刘楠,丛黎明,等.改进的FilmplateTM纸片法与国标法检测大肠菌群数的比较[J].解放军预防医学杂志,2015,33(01):14-17.
[7]段学辉,魏斌,傅奇.乳品中大肠菌群快速检测片的研究[J].中国乳品工业,2011,39(1):28-44.
[8]Williams M G,Hesselroth K E,Rajagopal R.Device for propagation and storage of microorganisms[P].United States Patent,2003.6649406.
[9]梁春梅,喻东威,吴玉秋,等.测试片法快速检测乳品中的菌落总数[J].中国乳品工业,2017,45(1):47-49.
[10]蔡尽忠.大肠菌群快速检测方法研究进展[J].生命科学仪器,2015,13(02)14-18.
[11]Niksic M,Mitrovic M,Obradovic D,et al.Role and comparison of ATP test with classical microbiological methods in hygiene design[J].Journal of Hygienic Engineering and Design,2012,1:62-65.
[12]周剑忠,黄明盛.生物荧光技术在乳品工业中的应用[J].中国乳品工业,2003,31(4):31-33.
[13]陆烨,胡国庆,陆龙喜.ATP生物荧光技术快速测定细菌总数的应用研究[J].中国消毒学杂志,2013,30(7):613-618.
[14]王惠荣,池勇志,辛丽花.ATP生物发光法在饮用水和再生水活菌生物量快速检测中的应用[J].给水排水,2016,42(4):27-33.
[15]王文彬.乳及乳制品中主要食源性致病菌的免疫快速检测方法研究[D].无锡:江南大学,2017.
[16]Zhao Y,Zhu A,Tang J,et al.Identification and measurement of staphylococcal enterotoxin M from Staphylococcus aureus isolate associated with staphylococcal food poisoning[J].Lett Appl Microbiol,2017,65(1):27-34.
[17]Arenas N E,Abril D A,Valencia P,et al.Screening food-borne and zoonotic pathogens associated with livestock practices in the Sumapaz region,Cundinamarca,Colombia[J].Trop Anim Health Prod.,2017,49(4):739-745.
[18]闫晗,吕国钦,张洋,等.乳品中3种常见细菌多重PCR检测方法的建立[J].食品与发酵工业,2015,41(7):149-154.
[19]Haugland R A,Varma M,Wymer L J,et al.Quantitative PCRanalysis of selected Aspergillus,Penicillium and Paecilomyces species[J].Systematic and Applied Microbiol,2004,27(2):198-210.
[20]Sartori C,Boss R,Ivanovic I,et al.Development of a new realtime quantitative PCR assay for the detection of Staphylococcus aureus genotype B in cow milk,targeting the new gene adlb[J].JDairy Sci,2017,S0022-0302(17):30701-30704.
[21]陈雪霞.食品微生物检验内容及检测技术研究[J].食品安全导刊,2017,6(18):109.
[22]Jimena Barrero-Canosa,Cristina Moraru,Laura Zeugner,et al.Direct-gene FISH:a simplified protocol for the simultaneous detection and quantification of genes and rRNA in microorganisms[J].Environmental Microbiology,2017,19(1):70-82.
[23]Gunasekera T S,Attfield P V,Veal D A.A flow cytometry method for rapid detection and enumeration of total bacteria in milk[J].Applied and Environmental Micorbiology[J],2005,66(3):1228-1232.
[24]Flint S,Walker K,Waters B,et al.Description and validation of a rapid(1 h)flow cytometry test for enumerating thermophilic bacteria in milk powders[J].J Appl Microbiol,2007,102(4):909-915.
[25]Hildebrandt P,Surmann K,Salazar M G,et al.Alternative fluorescent labeling strategies for characterizing gram-positive pathogenic bacteria:Flow cytometry supported counting,sorting,and proteome analysis of Staphylococcus aureus retrieved from infected host cells[J].Cytometry A,2016,89(10):932-940.
[26]杨莉婷,何丽,何海宁,等.流式细胞术对生鲜乳中微生物检测的应用研究[J].广西师范大学学报,2017,35(2):112-116.
[27]Langerhuus S N,Ingvartsen K L,Bennedsgaard T W,et al.Gram-typing of mastitis bacteria in milk samples using flow cytometry[J].J Dairy Sci,2013,96(1):267-277.
[2]池慧芳,高丽霞,郭爱萍.浅议乳品微生物检测技术[J].农产品加工(学刊),2011,10:95-97.
[3]张爱萍,孟瑞锋,侯式娟,等.阻抗法快速分析牛奶中的菌落总数[J].中国食品学报,2012,12(02):158-164.
[4]Bancalari E,Bernini V,Bottari B,et al.Application of Impedance Microbiology for Evaluating Potential Acidifying Performances of Starter Lactic Acid Bacteria to Employ in Milk Transformation[J].Front Microbiol,2016,17(7):1628.
[5]杜寒春,叶开富,刘绍刚.电阻抗法快速测定巴氏杀菌牛乳中菌落总数[J].中国乳品工业,2017,45(4):56-61.
[6]马秋霞,刘楠,丛黎明,等.改进的FilmplateTM纸片法与国标法检测大肠菌群数的比较[J].解放军预防医学杂志,2015,33(01):14-17.
[7]段学辉,魏斌,傅奇.乳品中大肠菌群快速检测片的研究[J].中国乳品工业,2011,39(1):28-44.
[8]Williams M G,Hesselroth K E,Rajagopal R.Device for propagation and storage of microorganisms[P].United States Patent,2003.6649406.
[9]梁春梅,喻东威,吴玉秋,等.测试片法快速检测乳品中的菌落总数[J].中国乳品工业,2017,45(1):47-49.
[10]蔡尽忠.大肠菌群快速检测方法研究进展[J].生命科学仪器,2015,13(02)14-18.
[11]Niksic M,Mitrovic M,Obradovic D,et al.Role and comparison of ATP test with classical microbiological methods in hygiene design[J].Journal of Hygienic Engineering and Design,2012,1:62-65.
[12]周剑忠,黄明盛.生物荧光技术在乳品工业中的应用[J].中国乳品工业,2003,31(4):31-33.
[13]陆烨,胡国庆,陆龙喜.ATP生物荧光技术快速测定细菌总数的应用研究[J].中国消毒学杂志,2013,30(7):613-618.
[14]王惠荣,池勇志,辛丽花.ATP生物发光法在饮用水和再生水活菌生物量快速检测中的应用[J].给水排水,2016,42(4):27-33.
[15]王文彬.乳及乳制品中主要食源性致病菌的免疫快速检测方法研究[D].无锡:江南大学,2017.
[16]Zhao Y,Zhu A,Tang J,et al.Identification and measurement of staphylococcal enterotoxin M from Staphylococcus aureus isolate associated with staphylococcal food poisoning[J].Lett Appl Microbiol,2017,65(1):27-34.
[17]Arenas N E,Abril D A,Valencia P,et al.Screening food-borne and zoonotic pathogens associated with livestock practices in the Sumapaz region,Cundinamarca,Colombia[J].Trop Anim Health Prod.,2017,49(4):739-745.
[18]闫晗,吕国钦,张洋,等.乳品中3种常见细菌多重PCR检测方法的建立[J].食品与发酵工业,2015,41(7):149-154.
[19]Haugland R A,Varma M,Wymer L J,et al.Quantitative PCRanalysis of selected Aspergillus,Penicillium and Paecilomyces species[J].Systematic and Applied Microbiol,2004,27(2):198-210.
[20]Sartori C,Boss R,Ivanovic I,et al.Development of a new realtime quantitative PCR assay for the detection of Staphylococcus aureus genotype B in cow milk,targeting the new gene adlb[J].JDairy Sci,2017,S0022-0302(17):30701-30704.
[21]陈雪霞.食品微生物检验内容及检测技术研究[J].食品安全导刊,2017,6(18):109.
[22]Jimena Barrero-Canosa,Cristina Moraru,Laura Zeugner,et al.Direct-gene FISH:a simplified protocol for the simultaneous detection and quantification of genes and rRNA in microorganisms[J].Environmental Microbiology,2017,19(1):70-82.
[23]Gunasekera T S,Attfield P V,Veal D A.A flow cytometry method for rapid detection and enumeration of total bacteria in milk[J].Applied and Environmental Micorbiology[J],2005,66(3):1228-1232.
[24]Flint S,Walker K,Waters B,et al.Description and validation of a rapid(1 h)flow cytometry test for enumerating thermophilic bacteria in milk powders[J].J Appl Microbiol,2007,102(4):909-915.
[25]Hildebrandt P,Surmann K,Salazar M G,et al.Alternative fluorescent labeling strategies for characterizing gram-positive pathogenic bacteria:Flow cytometry supported counting,sorting,and proteome analysis of Staphylococcus aureus retrieved from infected host cells[J].Cytometry A,2016,89(10):932-940.
[26]杨莉婷,何丽,何海宁,等.流式细胞术对生鲜乳中微生物检测的应用研究[J].广西师范大学学报,2017,35(2):112-116.
[27]Langerhuus S N,Ingvartsen K L,Bennedsgaard T W,et al.Gram-typing of mastitis bacteria in milk samples using flow cytometry[J].J Dairy Sci,2013,96(1):267-277.