水稻恶苗病菌对咪鲜胺的敏感性及生物学特性的研究
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摘要
为了明确黑龙江省水稻恶苗病菌对咪鲜胺的产生抗性情况,利用菌丝生长速率法检测了来自黑龙江省8个县(市)的32个恶苗病菌株对咪鲜胺的敏感性,确定敏感基线值为0.068 9μg·m L-1,有15.63%供试菌株为低抗菌株,3.12%为中抗菌株,没有检测到高抗菌株,表明黑龙江省水稻恶苗病菌对咪鲜胺的敏感性明显下降,已经产生抗性菌株。比较了抗性菌株JMS-3和敏感菌株NH-1的生物学特性,发现抗性菌株在生物学特性方面发生了一定变化,抗性菌株JMS-3菌丝生长速度明显低于敏感菌株NH-1,敏感菌株NH-1最适pH为7,抗性菌株JMS-3最适pH为6,敏感菌株NH-1最适氮源为硝酸钠,抗性菌株JMS-3最适氮源为硝酸钾。
In order to clarify Fusarium fujikuroi resistant to prochloraz,the mycelium growth rate method was used to test sensitivity of 32 Fusarium fujikuroi isolates to prochloraz that collected from 8 countries(cities) in Heilongjiang province,and the sensitive baseline value was 0.068 9 μg·m L~(-1). The low level resistant strains and the medium level resistant strains accounted for 15.63% and3.12%,respectively,and the high level resistant strains was not detected. The results showed that the sensitivity of Fusarium fujikuroi to prochloraz in Heilongjiang province was significantly lower and resistant strains was produced. Compared the biological characteristics of resistant strains JMS-3 and sensitive strains NH-1,the some changes had taken place in the biological characteristics of resistant strains,and resistant strains JMS-3 mycelium growth rate was significantly lower than sensitive strains NH-1. The optimal pH of sensitive strains NH-1 was 7 and the optimal p H of resistant strains JMS-3 was 6. The optimal nitrogen sources for sensitive strains NH-1 was NaNO_3 and the optimal nitrogen sources for resistant strains JMS-3 was KNO_3.
In order to clarify Fusarium fujikuroi resistant to prochloraz,the mycelium growth rate method was used to test sensitivity of 32 Fusarium fujikuroi isolates to prochloraz that collected from 8 countries(cities) in Heilongjiang province,and the sensitive baseline value was 0.068 9 μg·m L~(-1). The low level resistant strains and the medium level resistant strains accounted for 15.63% and3.12%,respectively,and the high level resistant strains was not detected. The results showed that the sensitivity of Fusarium fujikuroi to prochloraz in Heilongjiang province was significantly lower and resistant strains was produced. Compared the biological characteristics of resistant strains JMS-3 and sensitive strains NH-1,the some changes had taken place in the biological characteristics of resistant strains,and resistant strains JMS-3 mycelium growth rate was significantly lower than sensitive strains NH-1. The optimal pH of sensitive strains NH-1 was 7 and the optimal p H of resistant strains JMS-3 was 6. The optimal nitrogen sources for sensitive strains NH-1 was NaNO_3 and the optimal nitrogen sources for resistant strains JMS-3 was KNO_3.
引文
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[2]产祝龙,丁克坚,檀根甲.水稻恶苗病的研究进展[J].安徽农业科学,2002,30(6):880-883.
[3]杨谦.植物病原菌抗药性分子生物学[M].2版.北京:科学出版社,2011.
[4]张亚玲,王宝玉,台莲梅,等.黑龙江省和吉林省稻瘟病菌种群多样性研究[J].黑龙江八一农垦大学学报,2014,26(1):1-4.
[5]陈夕军,卢国新,童蕴慧,等.水稻恶苗病菌对三种浸种剂的抗性及抗药菌株的竞争力[J].植物保护学报,2007,34(4):425-430.
[6]兰波,李湘民.江西省稻瘟病菌对富士一号的抗药性研究[J].江西农业大学学报,2007,29(3):351-355.
[7]袁杰,杨学辉.贵州省稻瘟病菌对三环唑敏感性研究[J].西南农业学报,2006,19(2):219-221.
[8]赵志华,张锡明,范洁茹,等.咪鲜胺对水稻恶苗病菌及其抗药突变体生长发育的影响[J].农药学学报,2007,9(3):251-256.
[9]高宇.水曲柳主要病虫害及其防治方法[J].长春大学学报,2014,24(10):1384-1388.
[10]杨红福,吉沐祥,姚克兵,等.水稻恶苗病菌对咪鲜胺产生的抗药性研究及治理[J].江西学报,2013,25(6):94-96.
[11]产祝龙.水稻恶苗病的防治机理及其病原菌抗药性的研究[D].合肥:安徽农业大学,2003.
[12]Khan T Z,G ili M A,Khan M G.Inheritance studies for bakanae disease of rice[J].Pakistan Journal of phytopathology,1999,11(2):181-182.