没食子酸对水稻细菌性条斑病防治作用的持效期
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摘要
利用高效液相色谱法(HPLC)检测没食子酸(gallic acid,简称GA)在水稻叶片上的残留时间,并在检测水稻叶片上残留量的同时接种病原菌,调查接种后水稻细菌性条斑病的发病情况,以确定GA在水稻叶片上对水稻细菌性条斑病防治作用的持效期。研究结果表明,GA不具有内渗作用,只残留在水稻叶片的表面。分别用100、200和400 mg/L的浓度处理叶片后,GA在叶片表面的残留时间分别为16、24和28 d。200 mg/L浓度处理叶片后,GA对水稻细菌性条斑病防治作用的持效期为16 d。研究结果可为有效使用GA防治水稻细菌性条斑病奠定基础。
In order to evaluate the persistence of gallic acid(GA) on bacterial leaf streak of rice, high performance liquid chromatography was used to test the residue of GA in the rice leaf, and the pathogens was inoculated to evaluate the persistence of GA on bacterial leaf streak of rice. The results showed that GA could not infiltrate into rice leaf, but could reside on the surface of the leaf. The residue persistence of GA on the leaf at the concentrations of 100 mg/L, 200 mg/L and 400 mg/L was 16 d, 24 d and 28 d, respectively. The persistence of GA on bacterial leaf streak of rice at the concentration of 200 mg/L was 16 d. The results provided a theoretical basis for the efficient application of GA for the control of bacterial leaf streak of rice.
In order to evaluate the persistence of gallic acid(GA) on bacterial leaf streak of rice, high performance liquid chromatography was used to test the residue of GA in the rice leaf, and the pathogens was inoculated to evaluate the persistence of GA on bacterial leaf streak of rice. The results showed that GA could not infiltrate into rice leaf, but could reside on the surface of the leaf. The residue persistence of GA on the leaf at the concentrations of 100 mg/L, 200 mg/L and 400 mg/L was 16 d, 24 d and 28 d, respectively. The persistence of GA on bacterial leaf streak of rice at the concentration of 200 mg/L was 16 d. The results provided a theoretical basis for the efficient application of GA for the control of bacterial leaf streak of rice.
引文
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[21] 郭永斌,田苗,王亚南,等,六种铜制剂对苹果树腐烂病菌抑制作用的持效期及影响因素[J].北方园艺,2017(16):61-67.
[22] 郭延蕊,梁晨,李宝笃,等.氟吡菌胺对大白菜霜霉病的作用方式和持效期[J].农药,2014,53(5):372-374.
[23] 李金堂,默书霞.玉米灰斑病药剂防治持效期研究[J].中国植保导刊,2013,33(5):55-56.
[24] 杨晓.天然产物MCA在植物病害防治中的生物活性及其作用机理的研究[D].淄博:山东理工大学,2009.
[2] 黄华英,梁盛铭,陈梅珍,等.水稻细菌性条斑病监测与综合防控技术规程[J].生物灾害科学,2017,40(1):58-60.
[3] 邱亮.茶条槭叶没食子酸提取分离工艺研究[D].吉首:吉首大学,2012.
[4] XU Tanye, WANG Zhonghuan, LEI Tianli, et al. New flavonoid glycosides from Sedum aizoon L.[J]. Fitoterapia, 2015, 101: 125-132.
[5] 汪莹莹.没食子酸、并没食子酸的分离、性能及应用硏究[D].合肥:安徽大学,2012.
[6] 代斌.五倍子提取物的体外抗氧化作用研究[D].西安:陕西师范大学,2009.
[7] CHIA Y C, RAJBANSHI R, CALHOUN C, et al. Anti-neoplastic effects of gallic acid, a major component of Toona sinensis leaf extract, on oral squamous carcinoma cells [J]. Molecules, 2010, 15(11): 8377-8389.
[8] LIANG Wenna, LI Xihai, LI Yachan, et al. Gallic acid induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 human chondrosarcoma cells [J]. International Journal of Oncology, 2014, 44(1): 91-98.
[9] LI Dan, LIU Zuojia, YUAN Ye, et al.Green synthesis of gallic acid-coated silver nanoparticles with high antimicrobial activity and low cytotoxicity to normal cells[J]. Process Biochemistry, 2015, 50(3): 357-366.
[10] SARJIT A, WANG Y, DYKES G A.Antimicrobial activity of gallic acid against thermophilic Campylobacter is strain specific and associated with a loss of calcium ions [J]. Food Microbiology, 2015, 46: 227-233.
[11] 郑雪花,杨君,杨跃辉.没食子酸药理作用的研究进展[J].中国医院药学杂志,2017,37(1):94-98.
[12] 滕李濛,宋卓芮,毕家豪,等.铜(Ⅱ)-没食子酸配合物的抑菌活性研究[J].广东化工,2018,45(8):54-55.
[13] 谭平,刘斯宇,周太平,等.没食子酸衍生物的合成及抑菌活性研究[J].西华大学学报(自然科学版),2018,37(2):108-112.
[14] 常连举,张宗和,黄嘉玲,等.没食子酸的制备与应用综述[J].生物质化学工程,2007,44(4):48-52.
[15] 李悦,范腕腕,袁高庆,等.3,4,5-三羟基苯甲酸甲酯的抑菌特性及其对番茄青枯病的田间防治作用[J].农药,2014,53(11):845-848.
[16] 乔桂双. 五种Strobilurin类杀菌剂对黄瓜霜霉病菌生物活性及其抗性风险[D]. 保定: 河北农业大学, 2009.
[17] 李青,张名位,张瑞芬,等.5种籼稻品种谷壳中游离态和结合态酚类物质含量及其抗氧化活性比较[J].中国农业科学,2012,45(6):1150-1158.
[18] 郭素一.萘乙酸(α-Naphthylacetic acid)在番茄和水稻中的残留检测研究[D].南京:南京农业大学,2008.
[19] 吴雪钗,于波涛,侯艾林,等.没食子酸稳定性研究[J].西南国防医药,2006,16(5):484-485.
[20] 荆才,罗志军,胡鹏刚,等.反萃取降低高纯单宁酸生产中没食子酸残留量的应用[J].中国酿造,2017(3):156-159.
[21] 郭永斌,田苗,王亚南,等,六种铜制剂对苹果树腐烂病菌抑制作用的持效期及影响因素[J].北方园艺,2017(16):61-67.
[22] 郭延蕊,梁晨,李宝笃,等.氟吡菌胺对大白菜霜霉病的作用方式和持效期[J].农药,2014,53(5):372-374.
[23] 李金堂,默书霞.玉米灰斑病药剂防治持效期研究[J].中国植保导刊,2013,33(5):55-56.
[24] 杨晓.天然产物MCA在植物病害防治中的生物活性及其作用机理的研究[D].淄博:山东理工大学,2009.