脱脂椰蓉粉亚临界水降解动力学及产物形态研究
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摘要
以脱脂椰蓉为研究对象,考察了不同亚临界水降解条件对其降解过程的影响,并测定了100～200℃,不同反应时间下降解产物中还原糖的含量。采用Saeman模型对实验数据进行模拟,建立脱脂椰蓉粉亚临界水降解反应动力学方程,得到亚临界水降解动力学参数;并对降解物进行形态学分析。结果表明,Seaman模型能较好的反映脱脂椰蓉粉亚临界水降解的过程,初步降解的反应活化能E_(a1)为35.94 kJ/mol,还原糖降解活化能E_(a2)为32.12 k J/mol,指前因子K_(10)为6.50×10~2min~(-1)、K_(20)为3.24×10~2min~(-1)。该降解过程在140℃、30 min能得到温度的高还原糖产物。脱脂椰蓉粉在亚临界水中降解过程中,形态随温度、时间变化而变化,随着温度的升高原料的转化率逐渐升高,原料由固态转化为液态部分越多,水解程度越大。扫描电镜结果表明,随着降解温度的升高和降解时间的延长,脱脂椰蓉粉的纤维结构破坏越显著。
Using the defatted coconut flour as raw material,the study investigated the influence of different subcritical water degradation conditions on the degradation process and determined the contents of reducing sugar in degradation products with different reaction times of 100 ～ 200 ℃. The model of Saeman was used to simulate the experimental data,and the kinetics equation of the subcritical water degradation reaction was established.Based on this,the kinetic parameters of subcritical water degradation and the morphological analysis of the degradation are obtained. The results showed that the Seaman model could better reflect the subcritical water degradation process of defatted coconut flour.The initial degradation energy of material E_(a1) was 35.94 kJ/mol and the reducing sugar degradation activation energy E_(a2) was 32.12 kJ/mol,respectively. And the pre-exponential factors K_(10) was 6.50 × 10~2 min~(-1),K_(20) was 3.24 × 10~2 min~(-1).The degradation process could obtain a high-reducing sugar product at a temperature of 140 ℃ for 30 min.During the degradation of defatted coconut powder in subcritical water,the morphology changed over temperature and time. With the temperature increasing,the conversion rate of the raw material increased gradually,the raw material also converted from the solid to the liquid,and the degree of hydrolysis was greater. The results of scanning electron microscopy showed that the fiber structure damage of defatted coconut flour was more significant with the increase of degradation temperature and the prolongation of degradation time.
Using the defatted coconut flour as raw material,the study investigated the influence of different subcritical water degradation conditions on the degradation process and determined the contents of reducing sugar in degradation products with different reaction times of 100 ～ 200 ℃. The model of Saeman was used to simulate the experimental data,and the kinetics equation of the subcritical water degradation reaction was established.Based on this,the kinetic parameters of subcritical water degradation and the morphological analysis of the degradation are obtained. The results showed that the Seaman model could better reflect the subcritical water degradation process of defatted coconut flour.The initial degradation energy of material E_(a1) was 35.94 kJ/mol and the reducing sugar degradation activation energy E_(a2) was 32.12 kJ/mol,respectively. And the pre-exponential factors K_(10) was 6.50 × 10~2 min~(-1),K_(20) was 3.24 × 10~2 min~(-1).The degradation process could obtain a high-reducing sugar product at a temperature of 140 ℃ for 30 min.During the degradation of defatted coconut powder in subcritical water,the morphology changed over temperature and time. With the temperature increasing,the conversion rate of the raw material increased gradually,the raw material also converted from the solid to the liquid,and the degree of hydrolysis was greater. The results of scanning electron microscopy showed that the fiber structure damage of defatted coconut flour was more significant with the increase of degradation temperature and the prolongation of degradation time.
引文
[1]许芳连.亚临界水椰糠降解动力学的研究[D].海口:海南大学,2013.
[2]宋彦博.椰蓉膳食纤维的制备与功能特性评价及其在面包中的应用[D].海口:海南大学,2017.
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[5]Chen Hai-ming,Fu Xiong,Luo Zhi-gang.Properties and extraction of pectin-enriched materials from sugar beet pulp by ultrasonic-assisted treatment combined with subcritical water[J].Food Chemistry,2015,168:302-310.
[6]刘慧屏,银建中,徐刚.超/亚临界水解技术在生物质转化中的应用研究进展[J].生物质化学工程,2010,44(1):51-57.
[7]Shigeru H,Jintana W.Extraction of defatted rice bran by subcritical water treatment[J].Biochemical Engineering Journal,2008,40:44-53.
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[9]李婧秋,白新鹏,刘海信,等.油棕果渣纤维亚临界水降解动力学[J].化学反应工程与工艺,2015,31(4):337-342.
[10]李幼梅.番木瓜籽粕在亚临界水中降解过程及产物抗氧化活性研究[D].海口:海南大学,2016.
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[12]李思蓓,解玉红,罗晶,等.秸秆预处理中木质纤维物质含量测定方法的研究进展[J].安徽农业科学2011,39(3):1620-1622,1626.
[13]杨宁,王伟明,姚琳,等.3,5-二硝基水杨酸法测定发酵型果露酒中总糖含量[J].中国酿造,2018,37(1):181-184.
[14]余强,庄新姝,袁振宏等.木质纤维素类生物质高温液态水预处理技术[J].化工进展,2010,29(11):2177-2182.
[15]刘学军,唐俏瑜,李十中,等.草酸水解甜高粱秸秆渣的Saeman动力学模型[J].化学工程,2010,38(5):79-82.
[16]张晶晶.再生植物纤维的超/亚临界水解糖化特性及动力学研究[D].广州:华南理工大学,2011.
[17]Duba K S,Casazza A A,Mohamed H B et al.Extraction of polyphenols from grape skins and defatted grape seeds using subcritical water:Experiments and modeling[J].Food and Bioproducts Processing,2015,94:29-38.
[18]Thomas I,Tim R.Semi-continuous liquid hot water pretreatment of rye straw[J].Journal of Supercritical Fluids,2009,48:238-246.
[19]S Honda,E Akao,S Suzuki,et al.High-performance liquid chromatography of reducing carbohydrates as strongly ultravioletabsorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives[J].Analytical Biochemistry,1989,180:351-357.
[20]寇巍,赵勇,闫昌国,等.膨化预处理玉米秸秆提高还原糖酶解产率的效果[J].农业工程学报,2010,26(11):265-269.
[21]Chanelle Gavin,Casparus J R Verbeek,Mark C Lay.Formation of secondary structures in protein foams as detected by synchrotron FT-IR[J].Polymer Testing,2019,73.
[22]赵玉丛,李利红.基于FT-IR技术的黄芪多糖快速鉴别研究[J].中国兽医杂志,2014,50(5):79-81.
[23]童晓滨,谢妤,宋卫军.联合法提取雷竹叶多糖及其红外光谱的研究[J].宜春学院学报,2010,32(8):30-31,154.
[24]王宏慧,李翠翠,罗双群,等.玛咖多糖的研究进展[J].食品研究与开发,2018(10):201-204,224.
[25]巩桂芬.用于制备还原糖的植物纤维素的预处理方法研究[D].哈尔滨:哈尔滨工业大学,2010.
[26]牟莉.微波辅助下木质纤维素降解与溶解过程的研究[D].长春:东北师范大学,2012.
[2]宋彦博.椰蓉膳食纤维的制备与功能特性评价及其在面包中的应用[D].海口:海南大学,2017.
[3]Hossein M,Lee K T,Subhash B,et al.Sub/supercritical water liquefaction of oil palm fruit press fiber for the production of biooil:Effect of solvents[J].Bioresource Technology,2010,101:7641-7647.
[4]王荣春,卢卫红,马莺.亚临界水的特性及其技术应用[J].食品工业科技,2013,34(8):373-377.
[5]Chen Hai-ming,Fu Xiong,Luo Zhi-gang.Properties and extraction of pectin-enriched materials from sugar beet pulp by ultrasonic-assisted treatment combined with subcritical water[J].Food Chemistry,2015,168:302-310.
[6]刘慧屏,银建中,徐刚.超/亚临界水解技术在生物质转化中的应用研究进展[J].生物质化学工程,2010,44(1):51-57.
[7]Shigeru H,Jintana W.Extraction of defatted rice bran by subcritical water treatment[J].Biochemical Engineering Journal,2008,40:44-53.
[8]赵岩.秸秆制乙醇的超临界亚临界组合预处理与水解研究[D].北京:清华大学,2009.
[9]李婧秋,白新鹏,刘海信,等.油棕果渣纤维亚临界水降解动力学[J].化学反应工程与工艺,2015,31(4):337-342.
[10]李幼梅.番木瓜籽粕在亚临界水中降解过程及产物抗氧化活性研究[D].海口:海南大学,2016.
[11]徐明忠,庄新姝,袁振宏,等.农业废弃物高温液态水水解动力学[J].过程工程学报,2008(5):941-944.
[12]李思蓓,解玉红,罗晶,等.秸秆预处理中木质纤维物质含量测定方法的研究进展[J].安徽农业科学2011,39(3):1620-1622,1626.
[13]杨宁,王伟明,姚琳,等.3,5-二硝基水杨酸法测定发酵型果露酒中总糖含量[J].中国酿造,2018,37(1):181-184.
[14]余强,庄新姝,袁振宏等.木质纤维素类生物质高温液态水预处理技术[J].化工进展,2010,29(11):2177-2182.
[15]刘学军,唐俏瑜,李十中,等.草酸水解甜高粱秸秆渣的Saeman动力学模型[J].化学工程,2010,38(5):79-82.
[16]张晶晶.再生植物纤维的超/亚临界水解糖化特性及动力学研究[D].广州:华南理工大学,2011.
[17]Duba K S,Casazza A A,Mohamed H B et al.Extraction of polyphenols from grape skins and defatted grape seeds using subcritical water:Experiments and modeling[J].Food and Bioproducts Processing,2015,94:29-38.
[18]Thomas I,Tim R.Semi-continuous liquid hot water pretreatment of rye straw[J].Journal of Supercritical Fluids,2009,48:238-246.
[19]S Honda,E Akao,S Suzuki,et al.High-performance liquid chromatography of reducing carbohydrates as strongly ultravioletabsorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives[J].Analytical Biochemistry,1989,180:351-357.
[20]寇巍,赵勇,闫昌国,等.膨化预处理玉米秸秆提高还原糖酶解产率的效果[J].农业工程学报,2010,26(11):265-269.
[21]Chanelle Gavin,Casparus J R Verbeek,Mark C Lay.Formation of secondary structures in protein foams as detected by synchrotron FT-IR[J].Polymer Testing,2019,73.
[22]赵玉丛,李利红.基于FT-IR技术的黄芪多糖快速鉴别研究[J].中国兽医杂志,2014,50(5):79-81.
[23]童晓滨,谢妤,宋卫军.联合法提取雷竹叶多糖及其红外光谱的研究[J].宜春学院学报,2010,32(8):30-31,154.
[24]王宏慧,李翠翠,罗双群,等.玛咖多糖的研究进展[J].食品研究与开发,2018(10):201-204,224.
[25]巩桂芬.用于制备还原糖的植物纤维素的预处理方法研究[D].哈尔滨:哈尔滨工业大学,2010.
[26]牟莉.微波辅助下木质纤维素降解与溶解过程的研究[D].长春:东北师范大学,2012.