铜胁迫对葡萄根系活性氧和抗氧化酶活性的影响
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摘要
以‘玫瑰香’‘泽香’葡萄为试材,分别使用不同浓度的Cu SO_4·5H_2O处理当年生扦插苗,研究不同浓度铜处理(0.5～2 mmol/L)对葡萄根系活性氧和抗氧化酶活性的影响,检测铜离子毒害模式。结果表明:随着铜浓度的增加,葡萄根系活力呈显著下降趋势,而电导率和丙二醛含量呈显著上升趋势,说明试验设置的铜浓度对葡萄根系产生伤害;根系中超氧化物歧化酶、过氧化物酶和过氧化氢酶活性以及活性氧和过氧化氢均呈"先高后低"的趋势,表明这几种抗氧化物对铜处理的响应存在差异性。两个品种对铜胁迫的反应呈现了基本一致的规律,但‘泽香’抗氧化酶活性和丙二醛含量对铜胁迫的响应比‘玫瑰香’更敏感。
Taking the one-year cutting-seedlings of two grape cultivars('Muscat Hamburg' and 'Zexiang') as test materials, then reactive oxygen species and antioxidant enzyme activity were measured under effects of different copper concentration with 1/2 Hoagland nutrient solution. Results showed that within the scope of 0-2 mmol/L copper stress,root activity declined signi?cantly; however, relative electric conductivity and MDA contents increased signi?cantly,indicating all copper treatments caused damage to roots. ROS, H_2O_2, SOD, POD and CAT presented a tendency to rise ?rst and then decline, indicating their copper stress responses were difference. The two grape cultivars presented basic consistent, but 'Zexiang' antioxidant enzyme activity and MDA contents under copper stress response more sensitive than 'Muscat Hamburg'.
Taking the one-year cutting-seedlings of two grape cultivars('Muscat Hamburg' and 'Zexiang') as test materials, then reactive oxygen species and antioxidant enzyme activity were measured under effects of different copper concentration with 1/2 Hoagland nutrient solution. Results showed that within the scope of 0-2 mmol/L copper stress,root activity declined signi?cantly; however, relative electric conductivity and MDA contents increased signi?cantly,indicating all copper treatments caused damage to roots. ROS, H_2O_2, SOD, POD and CAT presented a tendency to rise ?rst and then decline, indicating their copper stress responses were difference. The two grape cultivars presented basic consistent, but 'Zexiang' antioxidant enzyme activity and MDA contents under copper stress response more sensitive than 'Muscat Hamburg'.
引文
[1]田淑芬,苏宏,聂松青.2018年中国鲜食葡萄生产及市场形势分析[J].中外葡萄与葡萄酒,2019(2):95-98.
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[3]SOMMER A L.Copper as an essential for plant growth[J].Plant Physiolology,1931,6(2):399-345.
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[5]段凯旋,杨洪强,冉昆,等.一氧化氮对铜、镉胁迫下平邑甜茶幼苗活性氧代谢的影响[J].中国农学通报,2007(10):104-110.
[6]管虹.铜离子对不同品种葡萄试管苗毒害机理的初步研究[D]山东师范大学,2009.
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[9]房经贵.葡萄应答铜胁迫分子机理的初步研究介绍[J].中外葡萄与葡萄酒,2015(4):73.
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[13]NEILL S J.Hydrogen peroxide and nitric oxide as signalling molecules in plants[J].Journal of Experimental Botany,2002,53(372):1237-1247.
[14]SANTONA L,CASTALDI P,MELIS P.Evaluation of the interaction mechanisms between red muds and heavy metals[J].Journal of Hazardous Materials,2006,136(2):324-329.
[15]李柏林,梅慧生.燕麦叶片衰老与活性氧代谢的关系[J].植物生理学报,1989,15(1):6-12.
[16]袁晓华,杨中汉.植物生理生化实验[M].北京:高等教育出版社,1983:128-133.
[17]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999:302-303
[18]ORT D R.A photoprotective role for O2 as an electron sink in photosynthesis?[J].Current Opinion in Plant Biology.20025(3):193-198.
[19]USHIMARU T,KANEMATSU S,KATAYAMA MAntioxidative enzymes in seedlings of Nelumbo nucifera germinated under water[J].Physiologia Plantarum,2010,112(1):39-46.
[20]杜秀敏,殷文璇,赵彦修.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121-125.
[21]苏梦云,范铭庆.渗透胁迫和钙处理对杉木幼苗膜脂过氧化及保护酶活性的影响[J].林业科学研究,2000,13(4):391-396.
[2]LIPMAN C B,MACKINNEY G.Proof of the essential nature of copper for higher green plants[J].Plant Physiology,1931,6(3):593-599.
[3]SOMMER A L.Copper as an essential for plant growth[J].Plant Physiolology,1931,6(2):399-345.
[4]李涛,蒲韵婷,王全华,等.Mn、Cu和Zn在植物生长发育中的生理作用[J].河北农业科学,2008(6):12-15.
[5]段凯旋,杨洪强,冉昆,等.一氧化氮对铜、镉胁迫下平邑甜茶幼苗活性氧代谢的影响[J].中国农学通报,2007(10):104-110.
[6]管虹.铜离子对不同品种葡萄试管苗毒害机理的初步研究[D]山东师范大学,2009.
[7]PIETRZAK U,MCPHAIL D C.Copper accumulation,distribution and fractionation in vineyard soils of Victoria Australia[J].Geoderma,2004,122(2/4):160-166.
[8]JACOBSON A R,DOUSSET S,GUICHARD N.Diuron mobility through vineyard soils contaminated with copper[J].Environmental Pollution,2005,138(2):250-259.
[9]房经贵.葡萄应答铜胁迫分子机理的初步研究介绍[J].中外葡萄与葡萄酒,2015(4):73.
[10]刘春生,史衍玺,马丽.过量铜对苹果树生长及代谢的影响[J].植物营养与肥料学报,2000,6(4):451-456.
[11]王爱国,罗广华.植物的超氧化物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990,6(1):55-57.
[12]PATTERSON B D,MACRAE E A,FERGUSON I B.Estimation of hydrogen peroxide in plant extracts using titanium(IV)[J]Analytical Biochemistry,1984,139(2):487-492.
[13]NEILL S J.Hydrogen peroxide and nitric oxide as signalling molecules in plants[J].Journal of Experimental Botany,2002,53(372):1237-1247.
[14]SANTONA L,CASTALDI P,MELIS P.Evaluation of the interaction mechanisms between red muds and heavy metals[J].Journal of Hazardous Materials,2006,136(2):324-329.
[15]李柏林,梅慧生.燕麦叶片衰老与活性氧代谢的关系[J].植物生理学报,1989,15(1):6-12.
[16]袁晓华,杨中汉.植物生理生化实验[M].北京:高等教育出版社,1983:128-133.
[17]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999:302-303
[18]ORT D R.A photoprotective role for O2 as an electron sink in photosynthesis?[J].Current Opinion in Plant Biology.20025(3):193-198.
[19]USHIMARU T,KANEMATSU S,KATAYAMA MAntioxidative enzymes in seedlings of Nelumbo nucifera germinated under water[J].Physiologia Plantarum,2010,112(1):39-46.
[20]杜秀敏,殷文璇,赵彦修.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121-125.
[21]苏梦云,范铭庆.渗透胁迫和钙处理对杉木幼苗膜脂过氧化及保护酶活性的影响[J].林业科学研究,2000,13(4):391-396.