西安市汉族人群两种稀有血型基因多态性分析
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摘要
目的研究西安地区汉族人群MNS、Duffy两个稀有血型系统基因多态性分布状况,为西安地区稀有血型库的建立及血液输注提供参考。方法使用序列特异性引物-聚合酶链式反应(SSP-PCR)方法,对300例西安市健康汉族人进行MNS、Duffy血型系统系统基因检测。结果西安市汉族人群MNS、Duffy稀有血型系统基因频率依次M=0.53,N=0.47,S=0.0817,s=0.9183;Fya=0.8033,Fyb=0.1967,χ~2检验分析结果发现符合Hardy-Weinberg平衡法则。结论西安市汉族人群2个稀有血型系统基因型呈现多态性分布,与国内其他地区相比较具有地域差异。
Objective:To study the gene polymorphism of MNS,Duffy blood groups in Han nationalities in Xi'an City for building the basis for human popμLation genetics,clinical use of blood or blood auto-transfusion and rare blood bank.Methods:Two blood groups genotyping of 300 adμLts from Xi'an City were detected by sequence specific primers-polymerase chain reaction(PCR-SSP) method and analyzed.ResμLts:The allele gene frequencies of rare blood groups in Han nationalities of Xi'an City were M=0.53,N=0.47,S=0.0817,s=0.9183;Fya=0.8033,Fyb=0.1967.ResμLts from chi-square test showed that the observed value and expected value of genotypes were in line with the law of Hardy-Weinberg equilibrium(P>0.05).Conclusion:The rare blood type system genotypes of Han popμLation in Xi'an city showed polymorphism distribution,which was compared with other regions in China.
Objective:To study the gene polymorphism of MNS,Duffy blood groups in Han nationalities in Xi'an City for building the basis for human popμLation genetics,clinical use of blood or blood auto-transfusion and rare blood bank.Methods:Two blood groups genotyping of 300 adμLts from Xi'an City were detected by sequence specific primers-polymerase chain reaction(PCR-SSP) method and analyzed.ResμLts:The allele gene frequencies of rare blood groups in Han nationalities of Xi'an City were M=0.53,N=0.47,S=0.0817,s=0.9183;Fya=0.8033,Fyb=0.1967.ResμLts from chi-square test showed that the observed value and expected value of genotypes were in line with the law of Hardy-Weinberg equilibrium(P>0.05).Conclusion:The rare blood type system genotypes of Han popμLation in Xi'an city showed polymorphism distribution,which was compared with other regions in China.
引文
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[2]Richard Onyuthi,Apecu Edgar M MμLogo,Fred Bagenda,et al.ABO and Rhesus(D)blood group distribution among blood donors in rural south western Uganda:a retrospective study[J].BMC Res Notes,2016,9:513-517.DOI:10.1186/s13104-016-2299-5.
[3]Sandler SG.Blood group genotyping:faster and more reliable identification of rare blood for transfusion[J].Lancet Haematol,2015,2:e270-1.
[4]郭建光,郭红岩.山东省泰安市无偿献血人群ABO、Rh、MN、P血型分布调查及其相关性分析[J].社区医学杂志,2005,3(12):19-21.
[5]李国良,肖莉,孙瑜,等.江西地区汉族人群ABO、Rh、MN、Kidd、Lewis、P血型系统调查[J].中国输血杂志,2001,14(增刊):96.
[6]L YAN,F ZHU,Q FU,et al.ABO,Rh,MNS,Duffy,Kidd,Yt,Scianna and Colton blood group systems in indigenous Chinese[J].Immunohematology Journal of Blood Group Serology and Education,2005,21:10-14.