基因组编辑技术在昆虫上的研究进展
摘要
基因组编辑技术是近年来发展出的一项对基因定向敲除与敲入的新技术,现行的基因编辑技术主要有人工核酸酶介导的锌指核酸酶(ZFNs)技术和转录激活样效应因子核酸酶(TALENs)技术及RNA介导的成簇间隔短回文重复系统(CRISPR/Cas9)等。这3种技术在基因编辑方面各有特色,该文就目前这3种主要基因编辑技术的特点、原理和方法,介绍了该技术在昆虫学上的应用,为未来昆虫领域的相关研究提供参考与借鉴。
引文
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[37]Sebo Z L,Han B L,Ying P,et al.A simplified and efficient germline-specific CRISPR/Cas9 system for genomic engineering[J].Fly,2013,8(1):52-57.
[38]Wang Y,Li Z,Xu J,et al.The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori[J].Cell Research,2013,23(12):1414-1416.
[39]Daimon T,Kiuchi T,Takasu Y.Recent progress in genome engineering techniques in the silkworm,Bombyx mor[iJ].Development Growth&Differentiation,2014,56(1):14-25.
[40]Ma S,Chang J,Wang X,et al.CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of Bm Ku70 in[J].Scientific Reports,2014,4(4):528-528.
[41]Robbins J.Twenty years of gene targeting:what we don't know[J].Circulation Research,2011,109(7):722-723.
[42]Ran F A,Hsu P,Lin C Y,et al.Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity[J].Cell,2013,154(6):1380-1389.
[43]Feng G,Xiao Z S,Feng J,et al.DNA-guided genome editing using the Natronobacterium gregoryi Argonaute[J].Nature Biotechnology,2016.
[44]Dong,Ren K,Qiu X,et al.The crystal structure of Cpf1 in complex with CRISPR RNA[J].Nature,2016,532(7600):522-526.
[45]Kim D,Kim J,Hur J K,et al.Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells[J].Nature Biotechnology,2016.
[46]Abudayyeh O O,Gootenberg J S,Konermann S,et al.C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector[J].Science,2016:5573.
[2]Consortium E P.An integrated encyclopedia of DNA elements in the human genome[J].Nature,2012,489(7414):57-74.
[3]Rong Y S,Golic K G.Gene targeting by homologous recombination in drosophila[J].Science Science,2000,288(5473):2013-2018.
[4]Rubin G M,Spradling A C.Genetic transformation of Drosophila with transposable element vectors[J].Science,1982,218(4570):348-353.
[5]Rio D C,Rubin G M.Identification and purification of a Drosophila protein that binds to the terminal 31-base-pair inverted repeats of the P transposable element[J].Proceedings of the National Academy of Sciences of the United States of America,1988,85(23):8929-8933.
[6]Carroll D,Morton J J,Beumer K J,et al.Design,construction and in vitro testing of zinc finger nucleases[J].Nature Protocol,2006,1(3):1329-1341.
[7]Klug A.The Discovery of Zinc Fingers and Their Applications in Gene Regulation and Genome Manipulation[J].Annual Review of Biochemistry,2010,79(1):213-231.
[8]Pabo C O,Peisach E,Grant R A.Design and selection of novel Cys2His2 zinc finger proteins[J].Annual Review of Biochemistry,2001,70(1):313-340.
[9]Wolfe S A,Nekludova L,Pabo C O.DNA recognition by Cys2His2 zinc finger proteins[J].Annual Review of Biophysics&Biomolecular Structure,2000,29(1):183-212.
[10]Sander J D,Cade L,Khayter C,et al.Targeted gene disruption in somatic zebrafish cells using engineered TALENs[J].Nature Biotechnology,2011,29(8):697-698.
[11]Ankit Gupta,Ryan G.Christensen,Amy L.Rayla,et al.An optimized two-finger archive for ZFN-mediated gene targeting[J].Nature Methods,2012,9(6):588-590.
[12]Townsend J A,Wright D A,Winfrey R J,et al.High frequency modification of plant genes using engineered zinc finger nucleases[J].Nature,2009,459(7245):442-445.
[13]Boch J,Scholze H,Schornack S,et al.Breaking the code of DNA binding specificity of TAL-type III effectors[J].Science,2009,326(5959):1509-1512.
[14]Moscou M J,Bogdanove A J.A Simple Cipher Governs DNA Recognition by TAL Effectors[J].Science,2009,326(5959):1501-1501.
[15]Boch J.TALEs of genome targeting[J].Nature Biotechnology,2011,29(2):135-136.
[16]Huang P,Xiao A,Zhou M,et al.Heritable gene targeting in zebrafish using customized TALENs[J].Nature Biotechnology,2011,29(8):699-700.
[17]Reyon D,Tsai S Q,Khayter C,et al.FLASH assembly of TALENs for high-throughput genome editing[J].Nature Biotechnology,2012,30(5):460-465.
[18]Gupta S K,John S,Naik R,et al.A guild of 45 CRISPRassociated(Cas)protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes[J].Plos Computational Biology,2005,1(6):e60.
[19]Garneau J E,Dupuis Mè,Villion M,et al.The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA[J].Nature,2010,468(7320):67-71.
[20]Friedland A E,Tzur Y B,Esvelt K M,et al.Heritable genome editing in C.elegans via a CRISPR-Cas9 system[J].Nature Methods,2013,10(8):741-743.
[21]Jinek M,Chylinski K,Fonfara I,et al.A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity[J].Science,2012,337(6096):816-821.
[22]Gasiunas G,Barrangou R,Horvath P,et al.Cas9-cr RNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(39):2579-2586.
[23]Cong L,Ran F A,Cox D,et al.Multiplex genome engineering using CRISPR/Cas systems[J].Science,2013,339(6121):819-823.
[24]Sander J D,Dahlborg E J,Goodwin M J,et al.Selectionfree zinc-finger-nuclease engineering by context-dependent assembly(Co DA)[J].Nature Methods,2011,8(1):67-69.
[25]Fu Y,Foden J A,Khayter C,et al.High frequency offtarget mutagenesis induced by CRISPR-Cas nucleases in human cells[J].Nature Biotechnology,2013,31(9):822-826.
[26]Hsu P D,Scott D A,Weinstein J A,et al.DNA targeting specificity of RNA-guided Cas9 nucleases[J].Nature Biotechnology,2013,31(9):827-832.
[27]Pattanayak V,Lin S,Guilinger J P,et al.High-throughput profiling of off-target DNA cleavage reveals RNAprogrammed Cas9 nuclease specificity[J].Nature Biotechnology,2013,31(9):839-843.
[28]Bibikova M,Golic M,Golic K G,et al.Targeted chromosomal cleavage and mutagenesis in drosophila using zinc-finger nucleases[J].Genetics,2002,161(3):1169-1175.
[29]Takasu Y,Kobayashi I,Beumer K,et al.Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease m RNA injection[J].Insect Biochemistry&Molecular Biology,2010,40(10):759-765.
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[31]Liu J,Li C,Yu Z,et al.Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy[J].Genet Genomics,2012,39(5):209-215.
[32]Watanabe T,Ochiai H,Sakuma T,et al.Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases[J].Nature Communications,2012,3(8):1017.
[33]Gratz S J,Cummings A M,Nguyen J N,et al.Genome Engineering of drosophila with the CRISPR RNA-Guided Cas9 Nuclease[J].Genetics,2013,194(4):1029-1035.
[34]Ren X,Sun J,Housden B E,et al.Optimized gene editing technology for drosophila melanogaster using germ line-specific Cas9[J].Proceedings of the National Academy of Sciences,2013,110(47):19012-19017.
[35]Bassett A R,Tibbit C,Ponting C P,et al.Highly Efficient Targeted Mutagenesis of drosophila,with the CRISPR/Cas9 System[J].Cell Reports,2013,4(1):1178-1179.
[36]Yu Z,Ren M,Wang Z,et al.Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in drosophila[J].Genetics,2013,195(1):289-291.
[37]Sebo Z L,Han B L,Ying P,et al.A simplified and efficient germline-specific CRISPR/Cas9 system for genomic engineering[J].Fly,2013,8(1):52-57.
[38]Wang Y,Li Z,Xu J,et al.The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori[J].Cell Research,2013,23(12):1414-1416.
[39]Daimon T,Kiuchi T,Takasu Y.Recent progress in genome engineering techniques in the silkworm,Bombyx mor[iJ].Development Growth&Differentiation,2014,56(1):14-25.
[40]Ma S,Chang J,Wang X,et al.CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of Bm Ku70 in[J].Scientific Reports,2014,4(4):528-528.
[41]Robbins J.Twenty years of gene targeting:what we don't know[J].Circulation Research,2011,109(7):722-723.
[42]Ran F A,Hsu P,Lin C Y,et al.Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity[J].Cell,2013,154(6):1380-1389.
[43]Feng G,Xiao Z S,Feng J,et al.DNA-guided genome editing using the Natronobacterium gregoryi Argonaute[J].Nature Biotechnology,2016.
[44]Dong,Ren K,Qiu X,et al.The crystal structure of Cpf1 in complex with CRISPR RNA[J].Nature,2016,532(7600):522-526.
[45]Kim D,Kim J,Hur J K,et al.Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells[J].Nature Biotechnology,2016.
[46]Abudayyeh O O,Gootenberg J S,Konermann S,et al.C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector[J].Science,2016:5573.